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数字共聚焦选项 – 2D 实时反卷积

图像反卷积算法可恢复宽场显微成像中的离焦荧光模糊信号

宽场显微镜是一种强大的工具，用于研究参与细胞信号转导的细胞和分子过程。尽管离焦光引起的荧光模糊是所有宽场显微镜的固有结果，但图像反卷积算法可以逆转这种离焦光伪影。

数字共聚焦选项是一项 2D 实时反卷积技术，可通过反算点扩散函数对图像进行数学去模糊处理，从而帮助修复细胞图像的分辨率和对比度。因此，可在保持定量信息的同时使图像和精细细节变得更加清晰。

*新*

保持检测质量的同时显著提高通量

数字共聚焦选项*增强了采集过程中图像的对比度，可使曝光时间缩短两倍以上，提高通量的同时保持检测质量。此外，对于Z’Factor（Z’ 对焦精确度）较低的样品，可提升Z'Factor 25% 以上。这种智能改善图像的过程使研究人员能够提高其通量，每小时可对超过 1000 万个细胞进行采集和分析。

该新模块为科学家提供了超快的检测速度和便利性，因为它为用户提供了实时反卷积功能，同时无需对图像进行后处理。对于致力于高内涵筛选的研究人员，资源和精力可专门用于筛选数百万份样本以寻找稀有的新药或基因疗法候选药物。

数字共聚焦选项专为 ImageXpress Pico 和 ImageXpress Micro 系统解决方案而设计。

采集和分析

专为 ImageXpress Pico 系统和 CellReporterXpress 图像采集和分析软件而设计

凭借数字共聚焦 2D on-the-fly 反卷积可显著提高分辨率和检测质量。数字共聚焦选项*可以减少曝光时间，同时保持观察结果的统计显著性。将数字共聚焦无缝集成至 ImageXpress Pico 系统的荧光图像采集工作流程中，可让您捕获信噪比更高的图像数据，从而进行更精确的分割和分析。

*ImageXpress Pico 数字共聚焦选项使用 AutoQuant 2D 实时反卷积。

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异硫氰酸荧光素 (FITC) 通道

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专为 ImageXpress Micro 系统和 MetaXpress 高内涵图像采集和分析软件而设计

全新的图像反卷积模块带来的优势，非常适合高内涵筛选的研究人员，资源和精力可专门用于筛选数百万份样本，以寻找稀有的新药或基因疗法候选药物。ImageXpress Micro 系统与用于 MetaXpress 软件的数字共聚焦选项相集成能够提供卓越易用的性能，同时还能提供快速和高质量图像。

数字共聚焦选项可实现可靠的图像分析

采集期间使用数字共聚焦选项的图像（底部）显示的细胞结构比不使用该选项的图像（顶部）更清晰，从而使图像分析更加可靠。

数字共聚焦选项可锐化荧光物体的强度峰

使用带数字共聚焦选项（顶部）和不带该选项（K 值 0.063；底部）的 ImageXpress Micro XL 系统采集含核旁斑的 HEK 293 细胞的图像。通过代表图像进行的行扫描的直方图显示了数字共聚焦选项如何锐化荧光物体的强度峰。当使用 MetaXpress 软件的 Granularity 应用模块分析数字共聚焦图像时，可以更准确地对核内颗粒进行计数（右图）。

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使用图像反卷积提升核转位试验检测结果

动态图像反卷积功能可以提高由于离焦光所导致的低信噪比，因此可用于检测需要精确识别不同细胞区室内的亚细胞结构或分子。我们利用 ImageXpress Pico 系统和 CellReporterXpress 软件，评估肿瘤坏死因子 α (TNF-α) 处理后核因子 κB (NF-κB) 从 HeLa 细胞的胞浆到胞核的转运。

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使用斑马鱼进行的高通量成像测定试验结果

ImageXpress® 系统可以提高较大视野下采集高质量图像的灵活性。MetaXpress® 软件让人能够通过简单的工作流程即可在各种应用中进行图像分析，与适用于数据挖掘的 AcuityXpress 信息学软件结合使用时，这种端到端解决方案可以极大提高斑马鱼体内筛选的通量。

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Cheryl Bell 谈论从手动显微镜到自动显微镜的过渡，我们的现场应用科学家 Cheryl Bell 最近出现在女性…...中

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CellReporterXpress 的数字共聚焦

数字共聚焦*选项专为 ImageXpress® Pico 自动细胞成像系统而设计，采用 CellReporterXpress® 图像采集和分析软件，可提供 2D…

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Improve nuclear translocation assay results using image deconvolution

使用图像反卷积提升核转位试验检测结果

宽场显微镜是一种强大的工具，用于研究参与细胞信号转导的细胞和分子过程。

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适用于 MetaXpress 的数字共聚焦

了解有关 ImageXpress Micro 成像系统的数字共聚焦选项的详细信息，其中该系统可在采集过程中增强图像对比度并缩短曝光时间以维持检测......

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High-throughput imaging assays using zebrafish, a model organism for human disease

使用斑马鱼（一种人类疾病的模型生物）进行高通量成像分析

近来，由于其成本、通量和可减少伦理争议的能力，斑马鱼模式筛选作为哺乳动物筛选的替代选择而备受亲睐。斑马鱼是一种非常有用的模型，适用于…

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数字共聚焦选项 – 2D 实时反卷积

图像反卷积算法可恢复宽场显微成像中的离焦荧光模糊信号

保持检测质量的同时显著提高通量

专为 ImageXpress Pico 系统和 CellReporterXpress 图像采集和分析软件而设计

专为 ImageXpress Micro 系统和 MetaXpress 高内涵图像采集和分析软件而设计

数字共聚焦选项可实现可靠的图像分析

数字共聚焦选项可锐化荧光物体的强度峰

应用说明

使用图像反卷积提升核转位试验检测结果

使用斑马鱼进行的高通量成像测定试验结果

数字共聚焦选项 – 2D 实时反褶积的资源

Get to Know our Field Applications Scientist: Cheryl Bell

了解我们的现场应用科学家：Cheryl Bell

ImageXpress Pico

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Digital Confocal for CellReporterXpress

CellReporterXpress 的数字共聚焦

Improve nuclear translocation assay results using image deconvolution

使用图像反卷积提升核转位试验检测结果

Digital Confocal for MetaXpress

适用于 MetaXpress 的数字共聚焦

High-throughput imaging assays using zebrafish, a model organism for human disease

使用斑马鱼（一种人类疾病的模型生物）进行高通量成像分析

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数字共聚焦选项 – 2D 实时反卷积

图像反卷积算法可恢复宽场显微成像中的离焦荧光模糊信号

保持检测质量的同时显著提高通量

专为 ImageXpress Pico 系统和 CellReporterXpress 图像采集和分析软件而设计

专为 ImageXpress Micro 系统和 MetaXpress 高内涵图像采集和分析软件而设计

数字共聚焦选项可实现可靠的图像分析

数字共聚焦选项可锐化荧光物体的强度峰

应用说明

使用图像反卷积提升核转位试验检测结果

使用斑马鱼进行的高通量成像测定试验结果

数字共聚焦选项 – 2D 实时反褶积的资源

数字共聚焦选项 – 2D 实时反褶积的相关产品

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