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SpectraMax L 化学发光读板机

在可升级的系统上进行高灵敏度和高通量的快反应和慢反应化学发光检测

 

 

SpectraMax® L 化学发光读板机为您的科研生涯铺平道路, 在 96 孔和 384 孔板中进行闪光和发光检测, 包括双荧光素酶报告基因、通过水母发光蛋白的 G 蛋白偶联受体 (GPCR)、生物发光共振能量转移 (BRET) 和吖啶酯闪光检测等。如果对通量有更高需求, SpectraMax L读板机可升级至多通道配置, 从而使通量提高6倍。SoftMax Pro GxP和其软件认证包提供完整的CD-ROM格式文档和大量的数据集, 可满足FDA 21 CFR Part 11 条款的合规要求。

优势

  • 降低检测下限的同时做到对低达 0.2fg 萤火虫荧光素酶(慢反应)或 20amol ATP(快反应)化学发光信号的高灵敏检测
  • 在 96 或 384 孔板体系上通过手动或自动切换光学孔径使空间干扰降到最低, 保证信号分辨能力
  • 专有的同步模拟和单光子计数能力可以提供超过 9 个数量级的动态检测范围, 确保对所有信号水平的检测

配件

  • 通过多通道探测器配置实现更高的样品通量
  • 通过配备双通道注射器进行双荧光素酶报告基因检测, 支持快反应检测如吖啶酯化学发光
  • 用户可通过安装滤光片做到对不同发射峰的区分, 进行 BRET 实验
  • 支持与 StakMax 微孔板堆板机无缝自动整合
  • 购买后通过添加检测器和注射器进一步对仪器进行升级, 及时满足您不断变化的实验室需求。

完整的系统

  • 支持从环境温度到 45°C 的稳定控制, 达到对温度敏感反应的监测和控制
  • 配备 Automix 用户编程的 4 种专有振板模式, 按照用户设置充分混匀微板孔中的样品
  • 使用新一代、比常规注射器需要更少维护的蠕动泵体系, 其支持每次仪器使用过后自动清洗注射器 , 以进一步减少注射器维护成本

全面的读板机验证

Molecular Devices 微孔读板机拥有最多样的验证工具, 实现客户各种各样的软硬件验证需求。Molecular Devices 通过以下产品和工具提供全面解决方案:

  • 对光吸收、荧光和化学发光模式进行硬件验证的 SpectraTest ABS1FL1LM1 验证板
  • SoftMax® Pro 软件验证包
  • 确保符合 FDA 联邦法规第 21 章第 11 款的软件工具

SpectraMax L 微孔板读板机支持多种快反应和慢反应分析测定类别, 包括:

  • 水母发光蛋白检测
  • ATP 检测
  • 生物发光共振能量转移 (BRET)
  • 细胞活性、增殖和细胞毒性
  • ELISA 和免疫分析
  • 酶学检测
  • 支原体监测
  • 报告基因检测

SoftMax Pro 软件是 Molecular Devices 出品的业界领先的一体化数据采集和分析软件, 它提供:

  • 丰富的模板库涵盖多种应用, 使您可以更迅速地开始读板机检测
  • 针对合规实验室可选 SoftMax Pro GxP 软件加速合规审批进程
  • 包括非线性拟合在内的等高阶计算分析, 以供深入分析数据和挖掘
  • 独有的 Mac 和 Windows 操作系统的应用支持, 以在不同操作系统间实现无缝数据交换
  • 可与领先的自动化和 LIMS 平台整合, 提供整合解决方案

一般参数

尺寸 (in.) 16.5(宽)×17(深)×8.75(高)
尺寸 (cm) 42(宽)×43(深)×22(高)
重量 36lbs (16.4kg)
电源 230Vac 50Hz, 115Vac 60Hz
检测器 低噪声光电倍增管, 具有同步模拟和单光子计数模式
微孔板格式 标准 96 和 384 孔微板, 微孔板高度范围 13.5-15.5mm

性能参数

孔间干扰(96 孔) 3×10-5
读板模式 终点法、动力学法、快速动力学法、双色读板
注射器 2 个可调量注射器 (10-100µl)
注射器准确度 ±1µL ±2%
注射器精确度 ±1µL ±2%
灵敏度(96 孔) < 20attomol ATP每孔, < 0.2fg 萤火虫荧光素酶每孔
波长范围 380-630nm
动态范围 9 个数量级
温度调节范围 温度范围:环境温度 +5°C 至 45°C

SpectraMax® L 读板机

产品

产品
编号

SpectraMax L 化学发光读板机 SpectraMax L
Config
BRET1 滤光片组 (480nm/535nm) 0310-5706
BRET2 滤光片组 (410nm/515nm) 0310-5707
条形码阅读器 0310-5743
SpectraTest LM1 化学发光验证板 0200-6186
StakMax 堆板机 STAKMAX
微孔读板机架 9000-0756

引用次数*:259

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Inhibition of GSK3β Stimulates BMP Signaling and Decreases SOST Expression Which Results in Enhanced Osteoblast Differentiation

MAE Schoeman, MJC Moester… - Journal of Cellular …, 2015 - Wiley Online Library
... After 24 hours of treatment with the indicated reagents, luciferase activity was determined
using the Dual-Glo Luciferase assay system (Promega, Fitchburg, WI, USA) with a
SpectraMax L luminometer (Molecular Devices, Sunnyvale, CA, USA). ...
 
 

Anti-metastatic immunotherapy based on mucosal administration of flagellin and immunomodulatory P10

FM de Melo, CJM Braga, FV Pereira… - Immunology and cell …, 2015 - nature.com
... The reaction was developed with ECL (EMD Millipore), read in Spectramax L equipment
(Molecular Devices) and expressed as Relative Luminescent Units. For immunoglobulin
isotyping, we used the protocol described by Bargieri et al. ...
 
 

Hydrogen‐Bonded Multilayers of Tannic Acid as Mediators of T‐Cell Immunity

V KozlovskayaB Xue, W Lei… - Advanced …, 2015 - Wiley Online Library
... of 200 μL. Luminescence was quantified using a SpectraMax L Luminescence
microplate reader and was recorded every 2 min for 1 h after stimulation with 100
ng mL −1 PMA and 1μg mL −1 In. Western Immunoblotting: Whole ...
 

Characterization of a Bispecific FLT3 X CD3 Antibody in an Improved, Recombinant Format for the Treatment of Leukemia

M Durben, D Schmiedel, M Hofmann, F Vogt… - Molecular …, 2015 - nature.com
... 6 hours. Luminescence induced after activation of the Jurkat cells by bispecific
antibodies bound to the NALM-16 cells was measured in a SpectraMax L luminometer
(Molecular Devices, Biberach, Germany). Serum concentrations ...
 
 

Glassy‐State Stabilization of a Dominant Negative Inhibitor Anthrax Vaccine Containing Aluminum Hydroxide and Glycopyranoside Lipid A Adjuvants

KJ HassettDJ VanceNK JainN Sahni… - Journal of …, 2015 - Wiley Online Library
... Cell viability was assessed using Cell Titer Glo (Promega, Madison, Wisconsin) and a
Spectramax L Luminometer (Molecular Devices). Neutralizing titers were defined as the
inverse titer that protected at least 50% of the cells from LT. RESULTS. ...
 
 

Stenotrophomonas maltophilia bloodstream infection in patients with hematologic malignancies: a retrospective study and in vitro activities of antimicrobial …

SY Cho, DG Lee, SM Choi, C Park… - BMC infectious …, 2015 - biomedcentral.com
... Relative luminescence units (RLU) were measured using a SpectraMax L luminescence
microplate reader (Molecular Devices, Sunnyvale, CA, USA). The percentage of RLUs compared
to the antibiotic-free controls (%RLU) was calculated, with minimal Page 6. ...
 

Elastin‐like recombinamers with acquired functionalities for gene‐delivery applications

MJ Piña, SM Alex, FJ Arias, M Santos… - … Research Part A, 2015 - Wiley Online Library
... Promega). The Bright Glo luciferase assay system (Promega) was added in a 1:1
proportion of lysate and luciferase reagent. The light produced was measured using
a luminometer (SpectraMax L, Molecular Devices). The protein ...
 

Calpain Determines the Propensity of Adult Hippocampal Neural Stem Cells to Autophagic Cell Death Following Insulin Withdrawal

KM Chung, H Park, S Jung, S Ha, SJ Yoo… - STEM …, 2015 - Wiley Online Library
... A freshly prepared dilution of the Calpain-Glo Reagent stock solution was added to the cells and
luminescence was recorded with a plate-reading luminometer (Molecular Devices, Sunnyvale,
CA, http://www.moleculardevices.com; SpectraMax L). The luminescence values ...
 
 

[HTML] Corosolic Acid Inhibits Hepatocellular Carcinoma Cell Migration by Targeting the VEGFR2/Src/FAK Pathway

CY Ku, YR Wang, HY Lin, SC Lu, JY Lin - 2015 - dx.plos.org
... 40 min. Finally, 50 μL kinase detection reagent was added to introduce luciferase
and samples were measured with a SpectraMax L Microplate reader (Molecular
Device, CA, USA). Rho GTPase activity assay. Huh7 cells were ...
 

Diurnal rhythms of plasma GLP-1 levels in normal and overweight/obese subjects: lack of effect of weight loss

JSG Muñoz, DJ Rodríguez, JJH Morante - Journal of physiology and …, 2015 - Springer
... levels of biological- ly active forms of GLP-1 (GLP17–36 amide and GLP17– 37) were measured
by fluorescence ELISA through the human glucagon-like peptide 1 (active) kit (Millipore, Billerica,
MA) with a luminescence multi-plate reader (SpectraMax L, Molecular Devices ...
 

* 截至 2015 年 6 月 26 日。来源:Google Scholar。