购物车

Gemini EM 荧光读板机

重新创造您的荧光检测

建立自定义荧光检测, 无需购买昂贵的滤光片组。配有双单色器的顶读和底读 Gemini EM 微孔板读板机, 可让您为自己的创新性荧光强度检测确定最佳激发和发射设置。如果尚不知晓最佳波长, Gemini EM 读板机可帮您确定。可方便地在顶读和底读模式之间切换, 以进行溶液和细胞分析测定。

优势

  • 在终点法、动力学法、光谱扫描和孔域扫描模式下支持 6 到 384 孔微孔板的样品检测
  • 通过双单色器在 250nm 到 850nm 之间选择激发和发射波长, 无需鉴定、购买和更换滤光片来优化波长
  • 在顶部和底部荧光读取之间进行切换, 以最佳光学灵敏度对溶液或细胞实验进行荧光测量
  • 对于终点法和动力学法测量, 可读取最多 4 对波长
  • 从微孔板孔中心的单个点到整个组织培养孔中的多个点进行孔域扫描, 报告出荧光测量结果

测量准确度和重复性

  • 使用 AutoPMT 系统的电子控制功能进行检测的优化, 并避免由于检测器饱和造成的高强度信号或由于重复读取产生的光漂白造成的弱信号的丢失
  • 与其他荧光酶标仪不同, Gemini XPS根据内部标准进行独有的校准, 对比样品间的相对荧光单位 (RFUs)
  • 从环境温度到 45°C 范围内保持稳定的温度调控, 以监测温度敏感性反应
  • 使用用户可设置的 Automix 功能, 充分混匀微孔板中的样品
  • 采用慢速进板设置选项, 减弱对微孔板中细胞或其他聚合物的震动干扰
  • 与 Molecular Devices 专为 SpectraMax 读板机设计的堆板机——StakMax 堆板机无缝整合, 以组合成自动化系统

全面的读板机验证

Molecular Devices 微孔读板机拥有最多样的验证工具, 实现客户各种各样的软硬件验证需求。Molecular Devices 通过以下产品和工具提供全面解决方案:

  • 对光吸收、荧光和化学发光模式进行硬件验证的 SpectraTest ABS1FL1LM1 验证板
  • SoftMax Pro 软件验证包
  • 确保符合 FDA 联邦法规第 21 章第 11 款的软件工具

Gemini EM 微孔板读板机支持多种荧光分析测定类别, 包括:

  • ELISA 和免疫分析
  • 核酸 (DNA) 定量
  • 蛋白定量
  • 报告基因检测
  • 细胞活力、增殖和细胞毒性
  • 酶学检测
  • 转运蛋白测定
  • 磷酸酶/激酶
  • 微生物生长

SoftMax® Pro 软件是目前使用最广泛的读板机控制和微孔板数据分析软件, 专为科研工作者的不同要求而设计。软件中含有预设实验模板、分析计算方法和21种不同的曲线拟合方式可选, 为数据获取直到数据分析、论文发表提供完整解决方案。FDA21CFR Part11合规工具可用于SoftMax Pro GxP 软件 , 为标准实验室提供完整解决方案以减少验证成本, 提供终端到终端的监管链, 无需额外的验证分析软件。

一般参数

尺寸 (in) 8.6(高)×22.8(宽)×15(深)
尺寸 (cm) 22(高)×58(宽)×38(深)
重量 35lbs (16kg)
电源 100-240VAC, 50/60Hz

性能参数

波长范围 - 激发 250-850nm
波长范围 - 发射 250-850nm
波长选择 单色器, 1.0nm 步进
波长带宽(激发和发射) 9nm
波长准确度 < ± 2.0nm
顶部读取灵敏度 3.0 fmol/孔 FITC 200µl, 96 孔
底部读取灵敏度 8.0 fmol/孔 FITC 200µl, 96 孔
光源 氙闪灯

读板时间

一般模式

96 孔 15 秒
384 孔 45 秒
动态范围 6 个数量级
温度范围 环境温度 +4°C, 最高 45°C
温度均一性(微孔板) ± 1°C @ 37°C

 

 Gemini™ EM 读板机

 

产品

货号

Gemini EM 荧光读板机 EM
SpectraTest FL1 荧光验证板 0200-5060
SpectraDrop™ 超微量检测板基础套装 0200-6262
SpectraDrop™ 超微量检测板 HTS 套装 0200-6267
微孔读板机架 9000-0756


 

引用次数*:1,340

最近引用:
如需完整列表, 请点击此处

[HTML] Paeonolum protects against MPP+-induced neurotoxicity in zebrafish and PC12 cells

XL Lu, YH Lin, Q Wu, FJ Su, CH Ye, L Shi… - BMC complementary …, 2015 - biomedcentral.com
... Quantitative measurements of fluorescence readings were determined at 485/538
nm (excitation/emission) using a SpectraMax GEMINI EM fluorescent plate reader
(Molecular Devices, USA) from cells in 96-well plates. The ...
 

Abnormalities in the Tricarboxylic Acid Cycle in Huntington Disease and in a Huntington Disease Mouse Model

NN Naseri, H Xu, J Bonica, JPG Vonsattel… - … of Neuropathology & …, 2015 - journals.lww.com
Close Window. Close Window. Enter your Email address: Wolters Kluwer Health may email
you for journal alerts and information, but is committed to maintaining your privacy and will
not share your personal information without your express consent. ...
 

Oxidative stress involving changes in Nrf2 and ER stress in early stages of Alzheimer's disease

SI Mota, RO Costa, IL Ferreira, I Santana… - … et Biophysica Acta (BBA …, 2015 - Elsevier
Oxidative stress and endoplasmic reticulum (ER) stress have been associated with Alzheimer's
disease (AD) progression. In this study we analyzed whether oxidati.
 
 

The Mechanism of Melanocytes-Specific Cytotoxicity Induced by Phenol Compounds Having a Prooxidant Effect, relating to the Appearance of Leukoderma

T Nagata, S Ito, K Itoga, H Kanazawa… - BioMed research …, 2015 - hindawi.com
... The fluorescence intensity of the medium containing the solubilized cells was measured at an
exciting wavelength of 485 nm and an emission wavelength of 530 nm with a fluorescence
microplate reader (Gemini EM fluorescence microplate reader) (Molecular Devices). ...
 

A Novel, Single Step, Highly Sensitive In-Vitro Cell-Based Metabolic Assay Using Honeycomb Microporous Polymer Membranes

S Velayudhan, PR Kumar… - Journal of Biomedical …, 2015 - ingentaconnect.com
... The release of FDA and PI embedded in the membrane was periodically monitored by carefully
pipetting 100 l of PBS into a 96-well black plate and subsequently quantifying the fluorescence
on a spectroflu- orimeter (SpectraMax Gemini EMMolecular Devices) equipped with ...
 

l-Histidine-based organoclays for the storage and release of therapeutic nitric oxide

AC Fernandes, ML PintoF Antunes… - Journal of Materials …, 2015 - pubs.rsc.org
... Then, 10 μL alamarBlue® was added directly to each well, the plates were incubated
at 37 °C for 4 h, and the fluorescence signal was then measured on a Spectra Max
Gemini EM from Molecular Devices with SoftMax Pro software. ...
 

The Janus face of α-toxin: a potent mediator of cytoprotection in staphylococci-infected macrophages

J Koziel, D Chmiest, D Bryzek, K Kmiecik… - Journal of innate …, 2015 - karger.com
... aliquots of supernatant into buffer (40 mM Pipes, 20% sucrose, 200 mM NaCl, 0.2% CHAPS,
2 mM EDTA) containing DEVD-AFC and recording increased fluorescence (λ ex = 350 nm, λ
em = 460 nm) for released AFC using a Spectra Max Gemini EM (Molecular Devices). ...
 

Thiol-mediated multiple mechanisms centered on selenodiglutathione determine selenium cytotoxicity against MCF-7 cancer cells

T Tobe, K Ueda, M Ando, Y Okamoto… - JBIC Journal of Biological …, 2015 - Springer
... Fluorescence of resazurin was recorded at an excitation wavelength of 544 nm and emission
wavelength of 612 nm using a plate reader (Gemini EMMolecular Devices, Sunnyvale, CA, USA).
Data were obtained from three independent experiments. Measurement of apoptosis ...
 

Selenoglycoproteins attenuate adhesion of tumor cells to the brain microvascular endothelium via a process involving NF-κB activation

JK Wrobel, JJ Choi, R Xiao, SY Eum… - The Journal of nutritional …, 2015 - Elsevier
... The adherence of calcein-labeled cancer cells was quantified by fluorescence measurements
(Gemini EM Fluorescence Microplate Reader; Molecular Devices, Sunnyvale, CA, USA) of
endothelial monolayers using an excitation of 485 nm and an emission of 530 nm. ...
 
 

Interference of doxycycline pretreatment in a model of abdominal aortic aneurysms

KM Mata, C Tefé-Silva, EM Floriano… - Cardiovascular …, 2015 - Elsevier
... CaCl 2 ). This activity was evaluated using a microplate fluorimeter (at λ excitation of 495nm,
λ emission of 515nm; Gemini EMMolecular Devices, Sunnyvale, CA, USA) after 120 min of
incubation at 37°C because the maximal dequenching of the substrate occurred at this time. ...
 

 

* 截至 2015 年 6 月 26 日。来源:Google Scholar。