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SpectraMax Plus 384 全波长光吸收读板机

迅速开始您的重要实验, 然后对各种类型的样品进行快速紫外/可见光吸收检测, 从而加快研究进程

对试管、比色皿和 96 或 384 孔微孔板中的样品迅速进行 190 至 1000 nm 的光吸收测量。SpectraMax® Plus 384 微孔板读板机集标准分光光度计和多种读板机应用于一身, 便捷易用。单色器波长可调消除了繁琐程序, 节省了您更换滤光片的时间。这款强大的读板机的典型应用包括:DNA 和 RNA 定量、酶动力学分析以及 ELISA 检测。当您的实验做好高通量分析准备后, 即可利用 StakMax® 微孔板的自动处理兼容性, 以与自动化系统充分整合。

优势

  • 非滤光片系统:含单色器, 最多可一次检测可见光区的 6 个波长, 扫描范围从 190-1000nm, 以 1nm 步进递增
  • 轻松优化测定:在终点法、动力学法和光谱扫描模式下, 读取从比色皿到 96 和 384 孔微孔板的各种样品格式
  • 采用唯一的非温度依赖的光程校正方法: PathCheck® 技术, 可对有差异的微孔板孔体积进行校正, 无需制作标准曲线
  • 使用窄的光吸收检测带宽, 以提高峰值信号检测的分辨率和准确度, 从而更好地进行实验信号鉴别。
  • 从环境温度到 45°C 范围内保持稳定的温度调控, 以监测温度敏感性反应
  • 使用可由用户设置的 Automix 功能充分混合微孔板孔中的样品
  • 使用 SpectraDrop 超微量板对低至 2µL 的 DNA、RNA 和蛋白质样品进行快速定量
  • 使用 SoftMax® Pro 软件进行全面的数据分析
  • StakMax® 堆板机无缝整合

 

 

微孔读板机验证

 

SpectraMax Plus384 微孔板读板机支持多种光吸收分析测定类别, 包括:

  • 细胞活性、增殖和细胞毒性
  • ELISA 和免疫分析
  • 内毒素检测
  • 酶学检测
  • 超微量检测
  • 微生物生长
  • 核酸 (DNA) 定量
  • 磷酸酶/激酶
  • 蛋白定量
  • 报告基因检测

 

SoftMax Pro 软件提供以下功能特点, 以便简化您的实验设定流程:

  • 设置简单和仪器自动识别
  • 预设实验模板, 用于终点法、动力学法、ELISA 蛋白定量, 例如Bradford、Lowry、BCA 和 DC 蛋白质检测实验
  • 多种检测类型:终点法、动力学法、光谱扫描法和*孔扫描法*
  • 标准品数据计算为默认设置
  • 数据自动恢复功能

Softmax Pro 软件数据分析

SoftMax Pro 软件提供多种自定义选项:

  • 针对常见数据分析功能的预设计算选项   
  • 自动导出数据到 Microsoft Excel
  • 可调整仪器设置和孔板读取区域, 以适合您的实验需求
  • 可进行客户数据计算设置, 用于优化光程选择
  • 提供完整的报告, 备注区域可灵活选择字体, 配有实时微型表格和图表, 简化您的结果分享体验     
  • 设有现代化的用户社区, 使我们的用户和技术专家之间可以更方便地进行方案交流和沟通
    可在我们大量用户之间共享专业意见

Softmax Pro 软件数据分析

 

  • 可与领先的自动化和 LIMS 平台整合, 提供合作解决方案
  • SoftMax Pro GxP 合规软件 为受管制实验室提供可选合规工具

 

 

* 可用于多种仪器型号

一般参数

尺寸 (in) 8.6(高)×22.8(宽)×15(深)
尺寸 (cm) 22(高)×58(宽)×38(深)
重量 30lbs (13.6kg)
电源 90-250VAC, 50/60Hz

性能参数

波长范围(nm) 190-1000nm
波长选择 单色器, 1.0nm 步进
带宽 ≤ 2.0nm
波长准确度 < ± 1.0nm
波长重复性 ± 0.2nm
OD检测范围 0.0 至 4.0 OD
分辨率 0.001 OD
准确度(微孔板) < ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
准确度(比色皿) < ± 0.005 OD ± 1.0%, 0.0 - 2.0 OD
OD精确度 ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
杂散光 ≤ 0.07%, 230nm
OD ≥ 3.15
光源 氙闪灯
读板时间 - 终点法 96 孔:9 秒
384 孔:29 秒
读板时间 - 动力学法 96 孔:间隔 9 秒
384 孔:间隔 29 秒
读板时间 - 快速读取模式 96 孔:5 秒
384 孔:16 秒
温度范围 环境温度 + 4°C, 最高 45°C
温度均一性(微孔板) ± 0.5°C @37°C

 

SpectraMax® Plus 384 微孔读板机

 

产品 货号
SpectraMax Plus 384 全波长光吸收读板机 PLUS 384
SpectraTest ABS1 光吸收验证板 0200-6117
StakMax 堆板机 StakMax
紫外光测量用 Quartz 96 孔微孔板 R8024
紫外光测量用 Quartz 96 孔半孔微孔板 R8028
SpectraDrop™ 超微量检测板基础套装 0200-6262
SpectraDrop™ 超微量检测板 HTS 套装 0200-6267
比色皿验证套件 0200-2420
微孔读板机架 9000-0756

引用次数*:8,230

最近引用:
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Middle-Aged Diabetic Females and Males Present Distinct Susceptibility to Alzheimer Disease-like Pathology

E Candeias, AI Duarte, I Sebastião… - Molecular …, 2016 - Springer
... Briefly, 10 μL of each brain cortical homogenate were added to 1 mL of Calimat
reagent and the absorbance read at 550 nm, 37 °C, in a SpectraMax Plus 384
multiplate reader (Molecular Devices, Wokingham, UK). Results ...
 

Turkish propolis supresses MCF-7 cell death induced by homocysteine

M Tartik, E Darendelioglu, G Aykutoglu… - Biomedicine & …, 2016 - Elsevier
... Later, WST-1 reagent (5 μl) was added to each well. After 4 h incubation, absorbance of each
well were measured at 450 nm (reference: 630 nm) by SpectraMax Plus 384 Microplate Reader
(Molecular Devices, USA). 2.4. Detection of lipid peroxidation. ...

PHLPP2 down regulation influences nuclear Nrf2 stability via Akt-1/Gsk3β/Fyn kinase axis in acetaminophen induced oxidative renal toxicity: Protection accorded by …

A Mathur, F Rizvi, P Kakkar - Food and Chemical Toxicology, 2016 - Elsevier
... bromide (MTT) at a final concentration of 5 mg/ml to the cells and incubated for 4 h. After removing
the supernatant media, 100% DMSO was added to dissolve formazan crystals and the absorbance
was measured at 530 nm on Spectramax plus 384 microplate based UV/visible ...

Phosphatase 2A Inhibition Affects Endoplasmic Reticulum and Mitochondria Homeostasis Via Cytoskeletal Alterations in Brain Endothelial Cells

AI PlácidoCMF Pereira, SC Correira, C Carvalho… - Molecular …, 2016 - Springer
... Mol Neurobiol Page 3. (SpectraMax Plus 384, Molecular Devices, San Francisco, CA, USA). ...
Caspase-like activi- ties were determined by measuring substrate cleavage at 405 nm in
a microplate reader (SpectraMax Plus 384, Molecular Devices). ...

Organophosphate Flame Retardants Act as Endocrine-Disrupting Chemicals in MA-10 Mouse Tumor Leydig Cells

G Schang, B Robaire, BF Hales - Toxicological Sciences, 2016 - Soc Toxicology
... Cellular viability assay. After treatment, cells were washed and incubated with MTT reagent for
4 h. Cells were lysed with 100 μL DMSO, and absorbance was read at 570 nM using a
SPECTRAmax Plus 384 (Molecular Devices, Sunnyvale, California). Cell counts. ...
 

 

Copper and hypoxia modulate transcriptional and mitochondrial functional-biochemical responses in warm acclimated rainbow trout (Oncorhynchus mykiss)

R Sappal, M Fast, S Purcell, N MacDonald… - Environmental …, 2016 - Elsevier
... gill mitochondria. The protein concentrations of liver and gill mitochondrial
suspensions were initially measured spectrophotometrically (Spectramax Plus 384,
Molecular Device, Sunnyvale, CA) by the Bradford (1976) method. ...

 

Aryl hydrocarbon receptor-dependent apoptotic cell death induced by the flavonoid chrysin in human colorectal cancer cells

SM Ronnekleiv-Kelly, M Nukaya, CJ Díaz-Díaz… - Cancer letters, 2016 - Elsevier
... Cell viability was measured using CellTiter-Fluor TM cell viability assay kit (Promega, Madison
WI). The fluorescence (excitation 390 nm, emission 460 nm) was detected using SpectraMax
Plus 384 microplate reader (Molecular Devices). Cytotoxicity and apoptosis assay. ...

Zinc supplementation leads to immune modulation and improved survival in a juvenile model of murine sepsis

HA Ganatra, BM Varisco, K Harmon, P Lahni… - Innate …, 2016 - ini.sagepub.com
... Color change was measured on a SpectraMax Plus 384 Microplate Reader (Molecular Devices,
Sunnyvale, CA, USA) using 412 nm absorbance, and the colorimetric change was used to
calculate MPO concentration in each sample as per the manufacturer's instructions. ...

The plastidial starch phosphorylase from rice endosperm: catalytic properties at low temperature

SK Hwang, S Singh, B Cakir, H Satoh, TW Okita - Planta, 2016 - Springer
... Absorbance was measured at 650 nm using a SpectraMax Plus 384 Microplate Reader
(Molecular Devices). ... Absorbance was measured every 2 min at 348 nm using a
SpectraMax Plus 384 Microplate Reader (Molecular Devices). ...
 

[HTML] Safety profile of the intravenous administration of brain-targeted stable nucleic acid lipid particles

M Conceição, L Mendonça, C NóbregaC Gomes… - Data in brief, 2016 - Elsevier
... How data was acquired, Microscope (Zeiss PALM Axiovert 200M), Real-time PCR system (Step
One Plus Real-Time PCR System, Applied Biosystems), Spectrophotometer (Spectramax Plus
384, Molecular Devices). Results were analyzed using GraphPad Prism software. ...
 

 

 

* 截至 2016 年 12 月 6 日。来源:Google Scholar。