优势
- 非滤光片系统：含单色器， 最多可一次检测可见光区的 6 个波长， 扫描范围从 340-850nm， 以 1nm 步进递增
- 轻松优化测定：在终点法或动力学法模式下对 96 和 384 孔板读取最多 6个波长， 可进行光谱扫描来确定波长峰值
- 采用唯一的非温度依赖的光程校正方法：PathCheck 技术， 可对有差异的微孔板孔体积进行校正， 无需制作标准曲线
- 使用可由用户设置的 Automix 功能充分混合微孔板孔中的样品
- 使用 SoftMax® Pro 软件进行全面的数据分析
- 与 StakMax® 堆板机无缝整合
微孔读板机验证
SpectraMax 340PC384 微孔读板机支持多种光吸收分析类别， 包括：
- 细胞活性、增殖和细胞毒性
- ELISA 和免疫分析
- 内毒素检测
- 酶学检测
- 微生物生长
- 磷酸酶/激酶
- 蛋白定量
- 报告基因检测
SoftMax Pro 软件提供以下功能特点， 以便简化您的实验设定流程：
- 设置简单和仪器自动识别
- 预设实验模板， 用于终点法、动力学法、ELISA 蛋白定量， 例如Bradford、Lowry、BCA 和 DC 蛋白质检测实验
- 多种检测类型：终点法、动力学法、光谱扫描法和*孔扫描法*
- 标准品数据计算为默认设置
- 数据自动恢复功能
SoftMax Pro 软件提供多种自定义选项：
- 针对常见数据分析功能的预设计算选项
- 自动导出数据到 Microsoft Excel
- 可调整仪器设置和孔板读取区域， 以适合您的实验需求
- 可进行客户数据计算设置， 用于优化光程选择
- 提供完整的报告， 备注区域可灵活选择字体， 配有实时微型表格和图表， 简化您的结果分享体验
- 设有现代化的用户社区， 使我们的用户和技术专家之间可以更方便地进行方案交流和沟通
可在我们大量用户之间共享专业意见
* 可用于多种仪器型号
|
一般参数
|
| 尺寸 (in) |
8.6 (h) x 22.8 (w) x 15 (d) |
| 尺寸 (cm) |
22 (h) x 58 (w) x 38 (d) |
| 重量 |
30lb (13.6kg) |
| 电源 |
90-250VAC， 50/60Hz |
|
性能参数
|
| 波长范围（nm） |
340 - 850nm |
| 波长选择 |
单色器， 1.0nm 步进 |
| 带宽 |
≤ 2.0nm |
| 波长准确度 |
± 1.0nm |
| 波长重复性 |
± 0.2nm |
| OD检测范围 |
0.0 至 4.0 OD |
| 分辨率 |
0.001 OD |
| 准确度 |
< ± 0.006OD ± 1.0%， 0.0-2.0OD |
| OD精确度 |
< ± 0.003OD ± 1.0%， 0.0-2.0OD |
| 杂散光 |
≤ 0.07%， 340nm
OD ≥ 3.15 |
| 光源 |
氙闪灯 |
| 读板时间 - 终点法 |
96 孔：9 秒
384 孔：29 秒 |
| 读板时间 - 动力学法 |
96 孔：间隔 9 秒
384 孔：间隔 29 秒 |
| 温度范围 |
环境温度 + 4°C， 最高 45°C |
| 温度均一性 |
± 0.5°C @37°C |
SpectraMax® 340PC384 微孔读板机
|
产品
|
货号
|
| SpectraMax 340PC384 全波长光吸收读板机 |
340PC 384 |
| SpectraTest ABS1 光吸收验证板 |
0200-6117 |
| StakMax 堆板机 |
StakMax |
| 微孔读板机架 |
9000-0756 |
引用次数*：9,100
最近引用：
如需完整列表， 请点击此处。
Y Honda, C Kamisato, Y Morishima - European Journal of Pharmacology, 2016 - Elsevier
... The reaction was initiated by adding 40 μl of a chromogenic substrate of FXa, S-2222 (750 μM).
The reaction velocity was measured with a SpectraMax Plus 340 (Molecular Devices Co,
Sunnyvale, CA, USA) at 405 nm from 2 min to 10 min at room temperature. ...
M Morimoto,
Y Matsuo, S Koide… - BMC …, 2016 - bmccancer.biomedcentral.com
... After 72 h of incubation, absorbance was measured at 450 nm in each well using a SpectraMax
340 spectrophotometer (Molecular Devices, CA, USA). ... After 72 h of incubation, absorbance was
measured at 450 nm in each well using a SpectraMax 340 spectrophotometer. ...
SK Afridi, MF Aftab, M Murtaza, S Ghaffar… - Biochemical and …, 2016 - Elsevier
... of URM-II-81 for 24 h at 37 °C with 5% CO 2 . MTT solution was added and cells were incubated
for 4 h. Formazan formed during incubation was solubilized by the addition of dimethyl sulfoxide
and quantified using ELISA reader (Molecular devices SpectraMax 340, USA). 2.5. ...
... Gaithersburg, MD). Absorbance of solubilized formazan product was quantified with
a SpectraMax 340 microplate reader at 570 nm using a reference wavelength of 630
nm (Molecular Devices, Sunnyvale, CA). NRF2 activation assays. ...
T Vila, K Ishida, SH Seabra, S Rozental - International Journal of …, 2016 - Elsevier
... The minimum concentrations that inhibited 50% and 90% of fungal growth (IC 50 and IC 90
values, respectively) were determined by visual analysis and by spectrophotometric readings
at 492 nm (SpectraMAX ® 340; Molecular Devices, Sunnyvale, CA). ...
HY Lee, HR Kim, SY Park… - BMC …, 2016 - bmccomplementalternmed. …
... AFC, Sigma-Aldrich) in a final volume of 100 μL and incubated at 37 °C. Caspase-3 activity was
analyzed continuously by monitoring fluorogenic AFC release at 37 °C. Subsequently, substrate
cleavage was monitored at 405 nm using a SPECTRAmax 340 microplate reader. ...
CA Pileggi, CP Hedges, SA Segovia… - Frontiers in …, 2016 - ncbi.nlm.nih.gov
... All assays were performed using the Molecular Devices Spectramax-340 96-well microplate
reading spectrophotometer at 25°C. CS and CPT1 activity were determined by measuring
absorbance at 412 nm in 50 mM Tris-HCl (pH 8.0) with 0.2 mM DTNB. ...
MR Delfina, D Tapfuma, S Mnkandla… - International Journal of …, 2016 - ccsenet.org
... The rate of production of a complex between thiocholine and DTNB was followed at 25ºC for
5 minutes at 412 nm using the SpectraMax 340 pc plate reader. ... The absorbance was measured
at 405 nm for 5 minutes at 25 °C using the SpectraMax 340 pc plate reader. ...
J Yao, DF Bruhn, MW Frank, RE Lee… - Journal of Biological …, 2016 - ASBMB
... The enzyme reactions were 100 μl in volume and performed and monitored in Costar UV
half-area 96-well plates with a SpectraMax 340 instrument taking 340-nm readings at 10-s
intervals at 37 °C. The substrate crotonyl-ACP was synthesized as described previously using ...
C Cortesia, T Bello, G Lopez, S Franzblau… - Brazilian Journal of …, 2016 - Elsevier
... Optical density at 600 nm (OD 600 ) was measured in a 96 well spectrophotometer (Molecular
Devices Spectramax 340), and fluorescence (485 nm excitation and 535 nm emission) was
measured in a 96 well plate fluorimeter (Molecular Devices SpectraMax, GeminisXS). ...
* 截至 2016 年 12 月 6 日。来源：Google Scholar。
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