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SpectraMax 340PC384 全波长光吸收读板机

检测 96 和 384 孔微孔板光吸收数据

 

使用针对 96 和 384 孔板的波长可调式可见光吸收读板机:SpectraMax® 340PC384 微孔读板机, 通过对各种研究问题进行解答, 可使您从中获得启发。可使用终点法、动力学法和光谱扫描法在 340-850nm 波长范围内采集数据, 采用 1nm 步进可调。我们的 SpectraMax® 340PC384 微孔读板机带有可调节至 45°C 的温度控制、与自动化系统兼容的接口以及 SoftMax® Pro 数据分析软件。PathCheck® 传感器技术会自动测量每个孔的液体深度, 并且还可用于计算浓度, 无需绘制标准曲线, 并可扩大读数的动态范围( 至 4.0 OD)。我们的 SpectraMax 340PC384 微孔读板机可运行许多应用, 包括 ELISA、细胞毒性、ß-半乳糖苷酶报告基因检测以及 NAD(P)H 检测等。

优势

  • 非滤光片系统:含单色器, 最多可一次检测可见光区的 6 个波长, 扫描范围从 340-850nm, 以 1nm 步进递增
  • 轻松优化测定:在终点法或动力学法模式下对 96 和 384 孔板读取最多 6个波长, 可进行光谱扫描来确定波长峰值
  • 采用唯一的非温度依赖的光程校正方法:PathCheck 技术, 可对有差异的微孔板孔体积进行校正, 无需制作标准曲线
  • 使用可由用户设置的 Automix 功能充分混合微孔板孔中的样品
  • 使用 SoftMax® Pro 软件进行全面的数据分析
  • StakMax® 堆板机无缝整合

 

微孔读板机验证

 

SpectraMax 340PC384 微孔读板机支持多种光吸收分析类别, 包括:

  • 细胞活性、增殖和细胞毒性
  • ELISA 和免疫分析
  • 内毒素检测
  • 酶学检测
  • 微生物生长
  • 磷酸酶/激酶
  • 蛋白定量
  • 报告基因检测

 

SoftMax Pro 软件提供以下功能特点, 以便简化您的实验设定流程:

  • 设置简单和仪器自动识别
  • 预设实验模板, 用于终点法、动力学法、ELISA 蛋白定量, 例如Bradford、Lowry、BCA 和 DC 蛋白质检测实验
  • 多种检测类型:终点法、动力学法、光谱扫描法和*孔扫描法*
  • 标准品数据计算为默认设置
  • 数据自动恢复功能

SoftMax Pro  软件设置

SoftMax Pro 软件提供多种自定义选项:

  • 针对常见数据分析功能的预设计算选项   
  • 自动导出数据到 Microsoft Excel
  • 可调整仪器设置和孔板读取区域, 以适合您的实验需求
  • 可进行客户数据计算设置, 用于优化光程选择
  • 提供完整的报告, 备注区域可灵活选择字体, 配有实时微型表格和图表, 简化您的结果分享体验     
  • 设有现代化的用户社区, 使我们的用户和技术专家之间可以更方便地进行方案交流和沟通
    可在我们大量用户之间共享专业意见

Softmax Pro 软件数据分析

 

  • 可与领先的自动化和 LIMS 平台整合, 提供合作解决方案
  • SoftMax Pro GxP 合规软件 为受管制实验室提供可选合规工具

 

 

* 可用于多种仪器型号

一般参数

尺寸 (in) 8.6 (h) x 22.8 (w) x 15 (d)
尺寸 (cm)   22 (h) x 58 (w) x 38 (d)
重量 30lb (13.6kg)
电源 90-250VAC, 50/60Hz

性能参数

波长范围(nm) 340 - 850nm
波长选择 单色器, 1.0nm 步进
带宽 2.0nm
波长准确度 ± 1.0nm
波长重复性 ± 0.2nm
OD检测范围 0.0 至 4.0 OD
分辨率 0.001 OD
准确度 < ± 0.006OD ± 1.0%, 0.0-2.0OD
OD精确度 < ± 0.003OD ± 1.0%, 0.0-2.0OD
杂散光 ≤ 0.07%, 340nm
OD ≥ 3.15
光源 氙闪灯
读板时间 - 终点法 96 孔:9 秒
384 孔:29 秒
读板时间 - 动力学法 96 孔:间隔 9 秒
384 孔:间隔 29 秒
温度范围 环境温度 + 4°C, 最高 45°C
温度均一性 ± 0.5°C @37°C

 

SpectraMax® 340PC384 微孔读板机

 

产品

货号

SpectraMax 340PC384 全波长光吸收读板机 340PC 384
 SpectraTest ABS1 光吸收验证板 0200-6117
StakMax 堆板机 StakMax
微孔读板机架 9000-0756

引用次数*:9,100

最近引用:
如需完整列表, 请点击此处

Prevention of arterial thrombosis by edoxaban, an oral factor Xa inhibitor in rats: monotherapy and in combination with antiplatelet agents

Y Honda, C Kamisato, Y Morishima - European Journal of Pharmacology, 2016 - Elsevier
... The reaction was initiated by adding 40 μl of a chromogenic substrate of FXa, S-2222 (750 μM).
The reaction velocity was measured with a SpectraMax Plus 340 (Molecular Devices Co,
Sunnyvale, CA, USA) at 405 nm from 2 min to 10 min at room temperature. ...
 

[HTML] Enhancement of the CXCL12/CXCR4 axis due to acquisition of gemcitabine resistance in pancreatic cancer: effect of CXCR4 antagonists

M Morimoto, Y Matsuo, S Koide… - BMC …, 2016 - bmccancer.biomedcentral.com
... After 72 h of incubation, absorbance was measured at 450 nm in each well using a SpectraMax
340 spectrophotometer (Molecular Devices, CA, USA). ... After 72 h of incubation, absorbance was
measured at 450 nm in each well using a SpectraMax 340 spectrophotometer. ...

A new glycotoxins inhibitor attenuates insulin resistance in liver and fat cells

SK Afridi, MF Aftab, M Murtaza, S Ghaffar… - Biochemical and …, 2016 - Elsevier
... of URM-II-81 for 24 h at 37 °C with 5% CO 2 . MTT solution was added and cells were incubated
for 4 h. Formazan formed during incubation was solubilized by the addition of dimethyl sulfoxide
and quantified using ELISA reader (Molecular devices SpectraMax 340, USA). 2.5. ...
 

[HTML] Developing a Gene Biomarker at the Tipping Point of Adaptive and Adverse Responses in Human Bronchial Epithelial Cells

JM CurrierWY Cheng, D Menendez, R Conolly… - PloS one, 2016 - journals.plos.org
... Gaithersburg, MD). Absorbance of solubilized formazan product was quantified with
SpectraMax 340 microplate reader at 570 nm using a reference wavelength of 630
nm (Molecular Devices, Sunnyvale, CA). NRF2 activation assays. ...

Miltefosine inhibits Candida albicans and non-albicans Candida spp. biofilms and impairs the dispersion of infectious cells

T Vila, K Ishida, SH Seabra, S Rozental - International Journal of …, 2016 - Elsevier
... The minimum concentrations that inhibited 50% and 90% of fungal growth (IC 50 and IC 90
values, respectively) were determined by visual analysis and by spectrophotometric readings
at 492 nm (SpectraMAX ® 340; Molecular Devices, Sunnyvale, CA). ...
 

[HTML] Porcine placenta hydrolysates regulate calcium disturbance in MC3T3-E1 osteoblastic cells

HY Lee, HR Kim, SY Park… - BMC …, 2016 - bmccomplementalternmed. …
... AFC, Sigma-Aldrich) in a final volume of 100 μL and incubated at 37 °C. Caspase-3 activity was
analyzed continuously by monitoring fluorogenic AFC release at 37 °C. Subsequently, substrate
cleavage was monitored at 405 nm using a SPECTRAmax 340 microplate reader. ...
 

[HTML] Maternal High Fat Diet Alters Skeletal Muscle Mitochondrial Catalytic Activity in Adult Male Rat Offspring

CA Pileggi, CP Hedges, SA Segovia… - Frontiers in …, 2016 - ncbi.nlm.nih.gov
... All assays were performed using the Molecular Devices Spectramax-340 96-well microplate
reading spectrophotometer at 25°C. CS and CPT1 activity were determined by measuring
absorbance at 412 nm in 50 mM Tris-HCl (pH 8.0) with 0.2 mM DTNB. ...
 

Toxicological Effects of Differently Polluted Dam Waters Spiked with Pesticides on Freshwater Snails Lymnaea Natalensis

MR Delfina, D Tapfuma, S Mnkandla… - International Journal of …, 2016 - ccsenet.org
... The rate of production of a complex between thiocholine and DTNB was followed at 25ºC for
5 minutes at 412 nm using the SpectraMax 340 pc plate reader. ... The absorbance was measured
at 405 nm for 5 minutes at 25 °C using the SpectraMax 340 pc plate reader. ...
 

Activation of exogenous fatty acids to acyl-acyl carrier protein cannot bypass FabI inhibition in Neisseria

J Yao, DF Bruhn, MW Frank, RE Lee… - Journal of Biological …, 2016 - ASBMB
... The enzyme reactions were 100 μl in volume and performed and monitored in Costar UV
half-area 96-well plates with a SpectraMax 340 instrument taking 340-nm readings at 10-s
intervals at 37 °C. The substrate crotonyl-ACP was synthesized as described previously using ...
 

[HTML] Use of green fluorescent protein labeled non-tuberculous mycobacteria to evaluate the activity quaternary ammonium compound disinfectants and antibiotics

C Cortesia, T Bello, G Lopez, S Franzblau… - Brazilian Journal of …, 2016 - Elsevier
... Optical density at 600 nm (OD 600 ) was measured in a 96 well spectrophotometer (Molecular
Devices Spectramax 340), and fluorescence (485 nm excitation and 535 nm emission) was
measured in a 96 well plate fluorimeter (Molecular Devices SpectraMax, GeminisXS). ...
 

 

 

* 截至 2016 年 12 月 6 日。来源:Google Scholar。