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SpectraMax 190 全波长光吸收读板机

一款探索生命奥秘的读板机

使用紫外/可见 SpectraMax 190 读板机进行 DNA 定量和进行终点法、动力学法以及光谱扫描法检测。针对96 孔板检测的多通道设计, 实现每个样品检测时都具有独立的检测光束和参比光束, 就像一个双光束分光光度计一样, 消除了因光纤输出的差异而导致的误差。通过单色器进行 190nm 到 850nm 的波长选择, 使您可以集中精力进行研究发现, 而无需追踪和更换滤光片。

 

专利的多通道光学设计的 SpectraMax 190 光吸收读板机模拟双光束分光光度计, 直接测量板中的每个样品, 消除了其他仪器上光纤输出的差异而引起的误差, 以得到更可靠的数据报告。

优势

  • 基于光栅的单色器, 可实现从 190nm 到 850nm 的紫外-可见波段的光吸收检测, 从而无需购买和更换滤光片即可优化波长
  • 针对 96 孔板, 在终点法或动力学法模式下都可以实现 6 个波长的光吸收检测, 并可以通过进行光谱扫描, 以确定波长峰值
  • 测量准确度的大大提升完全得益于其超窄的带宽, 并可以提高整个检测线性范围, 包括紫外波段的 DNA 定量检测
  • 使用窄的光吸收检测带宽, 以提高峰值信号检测的分辨率和准确度, 从而更好地进行实验信号鉴别
  • 使用 SpectraDrop 超微量板对低至 2µL 的 DNA、RNA 和蛋白质样品进行快速定量

特点

  • 从环境温度到 45°C 范围内保持稳定的温度调控, 可监测温度敏感性反应
  • 采用唯一的非温度依赖的光程校正方法:PathCheck®技术, 对有差异的微孔板孔体积进行校正, 无需制作标准曲线
  • 使用用户可设置的 Automix 功能, 充分混匀微孔板中的样品
  • 可与 Molecular Devices 公司专为 SpectraMax 读板机设计的堆板机 StakMax 进行无缝整合, 以组合成自动化工作站

 

全面的读板机验证

 

Molecular Devices 微孔读板机拥有最多样的验证工具, 实现客户各种各样的软硬件验证需求。Molecular Devices 通过以下产品和工具提供全面解决方案:

  • 对光吸收、荧光和化学发光模式进行硬件验证的 SpectraTest ABS1FL1LM1 验证板
  • SoftMax Pro 软件验证包
  • 确保符合 FDA 联邦法规第 21 章第 11 款的软件工具

SpectraMax 190 微孔板读板机支持多种光吸收检测类别, 包括:

  • 细胞活性、增殖和细胞毒性
  • ELISA 和免疫分析
  • 内毒素检测
  • 酶学检测
  • 微生物生长
  • 超微量检测
  • 核酸 (DNA) 定量
  • 磷酸酶/激酶
  • 蛋白定量
  • 报告基因检测

 

SoftMax Pro 软件提供以下功能特点, 以便简化您的实验设定流程:

  • 设置简单和仪器自动识别
  • 预设实验模板, 用于终点法、动力学法、ELISA 蛋白定量, 例如Bradford、Lowry、BCA 和 DC 蛋白质检测实验
  • 多种检测类型:终点法、动力学法、光谱扫描法和*孔扫描法*
  • 标准品数据计算为默认设置
  • 数据自动恢复功能

SoftMax Pro  软件设置

SoftMax Pro 软件提供多种自定义选项:

  • 针对常见数据分析功能的预设计算选项   
  • 自动导出数据到 Microsoft Excel
  • 可调整仪器设置和孔板读取区域, 以适合您的实验需求
  • 可进行客户数据计算设置, 用于优化光程选择
  • 提供完整的报告, 备注区域可灵活选择字体, 配有实时微型表格和图表, 简化您的结果分享体验     
  • 设有现代化的用户社区, 使我们的用户和技术专家之间可以更方便地进行方案交流和沟通
    可在我们大量用户之间共享专业意见

Softmax Pro 软件数据分析

 

  • 可与领先的自动化和 LIMS 平台整合, 提供合作解决方案
  • SoftMax Pro GxP 合规软件 为受管制实验室提供可选合规工具

 

 

* 可用于多种仪器型号

一般参数

尺寸 (in) 8.6(高)×22.8(宽)×15(深)
尺寸 (cm) 22(高)×58(宽)×38(深)
重量 30lbs (13/66kg)
电源 90-250VAC, 50/60Hz

性能参数

波长范围(nm) 190-850nm
波长选择 单色器, 1. 0nm步进
带宽 2.0nm
波长准确度 ± 1.0nm
波长重复性 ± 0.2nm
OD检测范围 0.0 至 4.0 OD
光度分辨率 0.001 OD
准确度 < ± 0.006OD ± 1.0%, 0.0-2.0OD
OD精确度 < ± 0.003OD ± 1.0%, 0.0-2.0OD
杂散光 ≤ 0.07%, 340nm
OD ≥ 3.15
光源 氙闪灯
读板时间 - 终点法 9 秒
读板时间 - 动力学法 最低间隔时间 9 秒
温度范围 环境温度 + 4°C, 最高 45°C
温度均一性 ± 0.5°C @37°C

 

 SpectraMax® 190 读板机

 

产品

货号

配有 SoftMax Pro 软件的 SpectraMax 190 读板机 190
SpectraTest ABS1 光吸收验证板 0200-6117
紫外光测量用 Quartz 96 孔板 R8024
紫外光测量用 Quartz 96 孔半体积板 R8028
SpectraDrop™ 超微量检测板基础套装 0200-6262
SpectraDrop™ 超微量检测板 HTS 套装 0200-6267
StakMax 堆板机 StakMax
微孔读板机架 9000-0756

 

引用次数*:8,720

最近引用:
如需完整清单, 请点击此处

 

Anti-oxidative and hepatoprotective effects of lithospermic acid against carbon tetrachloride-induced liver oxidative damage in vitro and in vivo

KWK Chan, WS Ho - Oncology reports, 2015 - spandidos-publications.com
... The plate was gently shaken, and the absorbance was measured at 540 nm on a microtiter plate
reader (SpectraMax 190; Molecular Devices). LDH assay. ... The absor- bance at 490 nm was
measured by a microtiter plate reader (SpectraMax 190; Molecular Devices). ...
 
 

[HTML] Intravaginal Lactic Acid Bacteria Modulated Local and Systemic Immune Responses and Lowered the Incidence of Uterine Infections in Periparturient Dairy …

Q Deng, JF Odhiambo, U Farooq, T Lam, SM Dunn… - 2015 - dx.plos.org
... tetramethylbenzidine (TMB). After adding the stop solution, the plate was read at
450 nm on a microplate spectrophotometer (Spectramax 190, Molecular Devices
Corporation, Sunnyvale, CA) within 10 min. The optical density ...
 

Measurement of total iron in Helicobacter pylori-infected gastric epithelial cells

SE Flores, AS Day, JI Keenan - BioMetals, 2015 - Springer
... H. pylori were resuspended in RPMI-1640 medium supplemented with 10 % (v/v) heat-inactivated
fetal bovine serum and bacterial numbers were quantitated by density, based on a standard curve
measured at an absorbance of 620 nm (SpectraMax ® 190, Molecular devices ...
 
 

[HTML] The anti-inflammatory and antioxidant activity of 25 plant species used traditionally to treat pain in southern African

SA Adebayo, JP Dzoyem, LJ Shai… - … and alternative medicine, 2015 - biomedcentral.com
... After incubation, the absorbance was read at 450 nm in a microplate reader (SpectraMax 190,
Molecular devices). Quercetin (10 mM) was used as a standard. ... The absorbance of the reaction
mixture was recorded at 593 nm (SpectraMax 190, Molecular devices) after 5 min. ...
 

DCLAK11, a multi-tyrosine kinase inhibitor, exhibits potent antitumor and antiangiogenic activity in vitro

X Guo, X Chen, L Tong, X Peng, M Huang… - Acta Pharmacologica …, 2015 - nature.com
... The reaction was terminated by the addition of 50 μL of 2 mol/LH 2 SO 4 as the color
changed, and the absorbance at 490 nm was analyzed using a SpectraMax 190
Microplate Reader (Molecular Devices, Sunnyvale, CA, USA). ...
 

Digestive enzyme activities are higher in the shortfin mako shark, Isurus oxyrinchus, than in ectothermic sharks as a result of visceral endothermy

KC Newton, J Wraith, KA Dickson - Fish physiology and biochemistry, 2015 - Springer
... well microplate to measure the change in absorbance of the sample (relative to a negative control
using 50 mM Tris–HCl homogenization buffer substituted for tissue supernatant) in a
SPECTRAmax 190 microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). ...
 
 

Superparamagnetic iron oxide nanoparticles exacerbate the risks of reactive oxygen species-mediated external stresses

C Luo, Y Li, L Yang, X Wang, J Long, J Liu - Archives of toxicology, 2015 - Springer
... spectrophotometer (SPECTRAmax 190, Molecular Devices Corporation, USA). ... C0016,
Beyotime Biotechnology) and was recorded at 490 nm using a microplate spectrophotom-
eter (SPECTRAmax 190, Molecular Devices Corporation, USA). ...
 
 

[HTML] BMI1 Is Expressed in Canine Osteosarcoma and Contributes to Cell Growth and Chemotherapy Resistance

MH Shahi, D York, R Gandour-Edwards, SS Withers… - PloS one, 2015 - dx.plos.org
... 2hrs. Absorbance at 490nm was measured with a spectrophotometer (Spectramax
190, Molecular Devices LLC). Each group contained 6 wells and mean absorbance
is expressed as a percentage of untreated control cells. ...
 
 

[HTML] Effects of Benzo [a] pyrene Exposure on Human Hepatocellular Carcinoma Cell Angiogenesis, Metastasis, and NF-κB Signaling

Q Ba, J Li, C Huang, H Qiu, J Li, R Chu… - Environmental health …, 2015 - ncbi.nlm.nih.gov
... The attached cells were incubated with CCK-8 in medium for 4 hr and quantified
by measuring the absorbance at 450 nm with a SpectraMax 190 microplate reader
(Molecular Devices, Sunnyvale, CA, USA). Animal husbandry. ...
 

Activation of endoplasmic reticulum stress is involved in the activity of icariin against human lung adenocarcinoma cells

S Di, C Fan, Y Yang, S Jiang, M Liang, G Wu, B Wang… - Apoptosis, 2015 - Springer
... the plates were incubated at 37 °C in a 5 % CO 2 /95 % air humidified incubator for 2 h. Then,
optical density (OD) values were assessed at 450 nm using a microplate reader (SpectraMax
190, Molecular Devices, USA), and the cell viability was expressed as the OD value [ 30 ]. ...
 

 

 

* as of June 26, 2015. Source: Google Scholar