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自动移液功能可确保一块微孔板上多种检测条件下的一致性

FlexStation® 3 多功能微孔读板机(酶标仪)可测定光吸收、荧光强度、荧光偏振、化学发光和时间分辨荧光。可编辑的移液功能适合于需要即时检测和自动化加样的生物化学和细胞检测。该款读板机(酶标仪)可使用移液系统从 96 孔或 384 孔板上额外转移 3 次检测试剂。

  • 满足更多检测条件

    满足更多检测条件

    自定义要注射到每个孔中的试剂 ,可减少试剂消耗并可实现在单个微孔板上探索更多的检测条件。

  • 自动化

    自动移液

    通过自动移液来提高检测质量并增加通量,尽可能的减少误差,从而在实验过程中和不同实验之间提供更严格的检测 CV。

  • 验证读板机(酶标仪)

    验证读板机(酶标仪)

    使用我们的 PathCheck® 功能来进行液体转移验证,以量化移液器移液性能。将其与 SoftMax® Pro 软件和 IQ/OQ/PQ 验证方案搭配使用,以符合 FDA 21 CFR 第 11 部分的要求。

FlexStation 3 多功能微孔板读板机(酶标仪)

FlexStation 3 多功能微孔板读板机(酶标仪)

特点

  • 移液头

    内置 8 通道移液头或 16 通道移液头可通过将 96 或 384 孔化合物板中的试剂转移至检测板来增加检测分析的灵活性。

  • 优化的激发光

    双光栅设计帮助用户选择合适的实验激发光和发射光波长并且避免不同实验间需要更换昂贵滤光片的麻烦。

  • 微量应用

    SpectraDrop™ 超微量检测板提供适用于小体积测定的高通量解决方案,可缩短样品制备时间并增加 DNA、RNA 和蛋白研究通量。

  • 兼容自动化设备

    FlexStation 3微孔板读板机(酶标仪)可与各种自动化设备兼容。我们主导“自动化系统供应商合作伙伴计划”使得我们的微孔板读板机(酶标仪)系统可以与所有世界领先的自动化系统快速、方便的进行整合。

经验证的 GxP 解决方案可确保数据完整性和合规性

我们为 GMP/GLP 实验室提供一整套经过验证的合规性解决方案,可以帮助您快速、自信地建立合规实验室。

  • 一流的微孔板读板机和洗板机可支持您的所有实验需求
  • IQ/OQ/PM 服务以数字化和合规格式保存仪器记录
  • 软件安装服务可验证并记录所需的组件是否按操作规范安装
  • 软件验证服务支持美国食品药品监督管理局 (FDA) 21 CFR Part 11 指南
  • 采用可追溯的验证板来检验您的微孔板读板机性能,帮助您得到可靠结果
适用于 GMP-GLP 实验室的 GxP 合规性解决方案

 

FlexStation 3 多功能微孔板读板机(酶标仪)的应用

  • 光吸收

    光吸收

    了解有关光吸收检测的所有信息 – 它如何运作、如何对它进行测定,以及如何使用它计算浓度。我们还提供有关常见光吸收检测的应用,包括 ELISA、核酸和蛋白定量,以及微生物生长的信息。

    了解更多信息 

    ELISA

    ELISA

    酶联免疫吸附检测 (ELISA) 通常以微孔板形式用于测定特定蛋白的含量,且大多时候均通过可见光波长范围内的吸光度来获得其结果。化学发光和荧光 ELISA 形式具有更高的灵敏度,可对少量待检测物质进行精确定量。

    了解更多信息 

  • 酶活性

    酶活性

    酶活性可使用显色底物来进行实时检测,在将显色底物添加至酶中时,它的颜色会发生变化,这种变化可在光吸收微孔板读板机(酶标仪)上检测。使用具有移液处理功能的仪器可自动添加底物,同时可检测反应,这样就能检测到最早期的反应部分,从而获得更加准确的结合亲和力估值。

    阅读应用说明 

    快速动力学钙流检测

    动力学钙流检测

    通过荧光染料测定细胞内钙的增加情况,从而监测 Gq 蛋白偶联受体 (GPCR) 活化情况。钙敏感染料会被吸收至细胞的胞质中。配体结合受体后,钙会被释放到细胞胞质中。该染料可结合细胞内的钙,随后可检测到荧光增强。

    阅读应用说明 

  • 荧光

    荧光

    了解有关荧光检测的所有信息 – 它是什么、它如何运作,以及如何使用仪器测定样本荧光强度。此外,还涵盖了许多荧光检测的应用,包括细胞活性、G 蛋白偶联受体活性,以及荧光核酸定量等。

    了解更多信息  

    荧光偏振 (FP)

    荧光偏振

    荧光偏振 (FP) 是一项广泛用于监测不断变化的结合事件的技术。其可用于评估包括蛋白-抗体结合和 DNA 杂交在内的生物分子相互作用以及酶活性,适用于基础研究以及高通量筛选。

    了解更多信息  

  • 发光

    发光

    了解发光检测 – 它是什么、它如何运作,以及发光检测与其他检测方式相比的优势。我们还提供关键发光检测,包括双荧光素酶报告基因、化学发光 ELISA、细胞毒性,以及生物发光共振能量转移 (BRET)。

    了解更多信息  

    报告基因检测

    SpectraMax DuoLuc 高分辨率报告基因检测

    报告基因检测使研究者能够确定基因是否表达以及表达水平如何。常被用于研究信号转导通路、基因调控、调节元件的结构等。一个报告基因载体包含一个启动子和报告基因或者目的基因和报告基因。例如萤火虫荧光素酶,该报告基因的表达可被轻松的检测到。将该载体转染到哺乳动物细胞中,当目标基因在细胞中表达时,报告基因也被表达,使用相应的检测试剂即可对报告基因的表达水平进行测定。

    阅读应用说明 

  • TRF、TR-FRET 和 HTRF

    时间分辨

    了解有关时间分辨荧光的所有信息 – TRF 和 TR-FRET,包括 HTRF 检测。我们将提供应用资源以协助您进行研究,包括激酶检测、细胞信号转导通路、蛋白间相互作用、细胞毒性等。

    了解更多信息  

FlexStation 3 多功能微孔板读板机(酶标仪)的技术参数和可选配置

 

*使用最低的设置和速度读取。

FlexStation 3 多功能微孔板读板机(酶标仪)的资源

报告内容
视频和网络研讨会
微孔板读板机,从 ELISA 到 AlphaScreen

从 ELISA 到 AlphaScreen,使用多功能读板机来扩展检测应用

FlexStation 3 多功能微孔板读板机(酶标仪)

FlexStation 3 多功能微孔板读板机(酶标仪)

  • Citation
    Dated: Jan 12, 2022
    Publication Name: Humana Press

    Ratiometric Ca2+ Measurements Using the FlexStation® Scanning Fluorometer

    Many commercial organizations currently use the Fluorometric Imaging Plate Reader (FLIPR®: Molecular Devices, Sunnyvale, CA) to conduct highthroughput measurements of intracellular Ca2+ concentration (see Chapter 7 ), taking advantage of its rapid kinetics, reliability, and compatibility for automation. For the majority of industrial applications… View more

    Many commercial organizations currently use the Fluorometric Imaging Plate Reader (FLIPR®: Molecular Devices, Sunnyvale, CA) to conduct highthroughput measurements of intracellular Ca2+ concentration (see Chapter 7 ), taking advantage of its rapid kinetics, reliability, and compatibility for automation. For the majority of industrial applications, the primary limitation of FLIPR (i.e., its requirement for single wavelength fluorescent probes using visible light excitation) is not a significant issue. Indeed, visible light probes offer certain benefits over their ultraviolet (UV)-excited ratiometric counterparts, such as reduced sample autofluorescence and higher absorbance, thereby allowing relatively low concentrations of dye to be used. However, under certain circumstances researchers may prefer to conduct high-throughput experiments with ratiometric dyes, particularly when issues of dye leakage, photobleaching, or signal-to-noise ratio become a concern.

    Contributors: Ian C. B. Marshall, Izzy Boyfield, Shaun McNulty  
    Go to article

  • Citation
    Dated: Aug 17, 2011
    Publication Name: Jove Journal

    Cell-based Calcium Assay for Medium to High Throughput Screening of TRP Channel Functions using FlexStation 3

    The Molecular Devices' FlexStation 3 is a benchtop multi-mode microplate reader capable of automated fluorescence measurement in multi-well plates. It is ideal for medium- to high-throughput screens in academic settings. It has an integrated fluid transfer module equipped with a multi-channel pipetter and the machine reads one column at a time to… View more

    The Molecular Devices' FlexStation 3 is a benchtop multi-mode microplate reader capable of automated fluorescence measurement in multi-well plates. It is ideal for medium- to high-throughput screens in academic settings. It has an integrated fluid transfer module equipped with a multi-channel pipetter and the machine reads one column at a time to monitor fluorescence changes of a variety of fluorescent reagents. For example, FlexStation 3 has been used to study the function of Ca2+-permeable ion channels and G-protein coupled receptors by measuring the changes of intracellular free Ca2+ levels. Transient receptor potential (TRP) channels are a large family of nonselective cation channels that play important roles in many physiological and pathophysiological functions. Most of the TRP channels are calcium permeable and induce calcium influx upon activation. In this video, we demonstrate the application of FlexStation 3 to study the pharmacological profile of the TRPA1 channel, a molecular sensor for numerous noxious stimuli. HEK293 cells transiently or stably expressing human TRPA1 channels, grown in 96-well plates, are loaded with a Ca2+-sensitive fluorescent dye, Fluo-4, and real-time fluorescence changes in these cells are measured before and during the application of a TRPA1 agonist using the FLEX mode of the FlexStation 3. The effect of a putative TRPA1 antagonist was also examined. Data are transferred from the SoftMax Pro software to construct concentration-response relationships of TRPA1 activators and inhibitors.

    Contributors: Jialie Luo, Yingmin Zhu, Michael X. Zhu, Hongzhen Hu  
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  • Citation
    Dated: Dec 15, 2005
    Publication Name: Journal of Neuroscience Methods

    FlexStation examination of 5-HT3 receptor function using Ca2+- and membrane potential-sensitive dyes: Advantages and potential problems

    The FlexStation is a 96 or 384 fluorescent plate reader with the capability of adding solutions during readings; it therefore has the potential to provide high throughput analyses of the functional characteristics of neurotransmitter-gated ion channels that can be examined using changes in fluorescence. The 5-HT3 receptor is one such protein, as… View more

    The FlexStation is a 96 or 384 fluorescent plate reader with the capability of adding solutions during readings; it therefore has the potential to provide high throughput analyses of the functional characteristics of neurotransmitter-gated ion channels that can be examined using changes in fluorescence. The 5-HT3 receptor is one such protein, as its activation results in a change in membrane potential due to the opening of a Ca2+-permeant, cation-selective channel; it can therefore be studied using both Ca2+- and membrane potential-sensitive fluorescent dyes. Here we have used the FlexStation to examine the function of recombinant 5-HT3 receptors expressed in HEK293 cells using both these classes of dye. The results show that the pharmacological characteristics of the receptor obtained using the FlexStation is similar to those reported using other functional methods, although caution must be applied when using the membrane potential dye as large changes in membrane potential can yield inaccurate EC50s. Modifying the constituents of the buffer, however, so that the change in membrane potential was reduced, yielded EC50 values that were similar to previously reported data. We conclude that the FlexStation is a useful tool for high throughput studies when examining the function of neurotransmitter receptors that result in either a change in Ca2+ concentration or membrane potential.

    Contributors: Kerry L .Price, Sarah C.R. Lummis  
    Go to article

FlexStation® 3 系列多功能微孔板读板机(酶标仪)

产品 货号
具备 SoftMax Pro 软件的 FlexStation 3 微孔板读板机(酶标仪)包括:FlexStation 3 基础系统, 1 年质保,涵盖部件和人工服务。**不包括 8 通道或 16 通道移液器吸头, 该移液器吸头必须单独购买,且必须在订购仪器时一起订购。 Flex3
SpectraDrop™ 超微量板基础版套装 0200-6262
SpectraDrop™ 超微量板 HTS 版套装 0200-6267
FlexStation 3 台式多功能酶标仪(读板机) IQ/OQ 验证方案 0112-0160

FlexStation 3 多功能微孔板读板机(酶标仪)的相关产品和服务