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8 个可选滤光片提供简化的检测设置

 

EMax® Plus 微孔板读板机(酶标仪)可在一个入门级平台中提供强大的解决方案。八个滤光片可实现蛋白定量、细胞活性和 ELISA 等可见波长光吸收测定应用。此款微孔板读板机(酶标仪)可测定平底/圆底 96 孔板,并通过每次读数前的光源自动校准来确保准确度。

  • 涵盖广泛应用

    涵盖广泛应用

    八个滤光片可满足各种应用的需求 — ELISA 和免疫测定;Bradford、Lowry、BCA 等蛋白定量以及 DC 蛋白检测;磷酸酶和激酶;细胞活性。

  • 简化的检测设置

    简化的检测设置

    SoftMax® Pro 软件通过预设模板、标准的数据计算设置、自动数据恢复,以及结果显示和分析来简化检测设置。

  • 自定义选项

    自定义选项

    自定义各种选项,例如用于暂停和恢复动力学读数的非连续动力学功能、用于常见数据分析功能的预定义计算选项,以及便捷的数据导出。

使用 EMax plus 微孔读板机(酶标仪)进行 ELISA 工作流程

使用 EMax plus 微孔板读板机(酶标仪)和 MultiWash+ 微孔板洗板机进行的 ELISA 工作流程

特点

  • 轻松显示数据

    包含强大的曲线拟合方案和统计分析功能,可轻松将采集到的数据显示为灰度图像或彩色图像、3D 图形、动力学平面图,或轻松显示反应速率。

  • 简洁设计

    占用空间小且外形小巧,节省了实验台空间。

EMax Plus 微孔板读板机(酶标仪)的应用

  • 吸光度

    吸光度

    了解有关光吸收检测的所有信息 – 它如何运作、如何对它进行测定,以及如何使用它计算浓度。我们还提供有关常见吸光度应用和检测的信息,包括 ELISA、核酸和蛋白定量以及微生物生长。

    了解更多信息 

    ELISA

    ELISA

    酶联免疫吸附检测 (ELISA) 通常以微孔板形式用于测定特定蛋白的含量,且大多数情况下均通过可见光波长范围内的吸光度来获得其结果。化学发光和荧光 ELISA 形式具有更高的灵敏度,可对少量待检测物质进行精确定量。

    了解更多信息 

  • 蛋白检测、定量和分析

    蛋白检测

    蛋白检测、定量和分析对于研究各种生物过程极为重要。从蛋白质纯化和标记到电泳样品制备的各个流程,蛋白质浓度测定均是必不可少的。蛋白质可以通过 280 nm 处的吸光度来直接定量,或使用比色法(BCA 法、Bradford 法等)或荧光法来间接定量,这些方法具有灵敏度高的优势。可使用 ELISA 检测并测量复杂样品(如血清或细胞裂解物)中的某种特定蛋白。

    了解更多信息 

    蛋白定量(Bradford、Lowry、BCA、DC)

    蛋白定量

    蛋白浓度可通过紫外分光光度计中 280nm 处的吸光度来直接测定,或使用 BCA 和 Bradford 检测等比色法来间接测定。由于不需要其他试剂,光吸收法定量非常简便,但比色测定法可在样品非常珍稀的情况下提供更佳的灵敏度,通常情况下此方法作为首选。两种方法都可在紫外可见分光光度计或光吸收功能的微孔读板机(酶标仪)中进行检测。

    阅读应用说明 

EMax Plus 微孔板读板机(酶标仪)的技术参数和可选配置

 

*使用最低的设置和速度读取。

EMax Plus 微孔板读板机(酶标仪)的资源

报告
视频和网络研讨会
使用 EMax plus 微孔板读板机(酶标仪)和 MultiWash+ 微孔板洗板机进行的 ELISA 工作流程

使用 EMax plus 微孔板读板机(酶标仪)和 MultiWash+ 微孔板洗板机进行的 ELISA 工作流程

  • Citation
    Dated: Dec 14, 2020
    Publication Name: Brain, Behavior, and Immunity

    Cytokine Dysregulation Associated with Exam Stress in Healthy Medical Students

    The mechanisms of stress-related immune alterations have not been fully elucidated. Cell-mediated immune responses as well as antibody and certain cytokines are reported as being suppressed during times of high stress. However, the role of suppression vs dysregulation has not been established in human stress models. The effect of exam stress on… View more

    The mechanisms of stress-related immune alterations have not been fully elucidated. Cell-mediated immune responses as well as antibody and certain cytokines are reported as being suppressed during times of high stress. However, the role of suppression vs dysregulation has not been established in human stress models. The effect of exam stress on regulatory cytokines in 16 healthy medical students was assessed by measuring type-1 (IFN-γ) and type-2 (IL-10) cytokines from 72-h PHA/PMA-stimulated PBMC 4 weeks before and 48 h after exams. Results demonstrated decreased IFN-γ accompanied by increased IL-10 during exam stress that resulted in a decreased IFN-γ:IL-10 ratio. There was a significant correlation between the cytokine response to PHA/PMA and number and subjective adjustment to daily hassles. Additionally, students who reported greater levels of loneliness also reported greater numbers of and poorer subjective adjustment to hassles. The differences were consistent in both males and females but did not correlate with AM cortisol levels. Additionally, when individuals were grouped into high vs low preexam hassle levels, the type-1/type-2 shift in the IFN-γ:IL-10 ratio occurred in the low hassles group only. These data suggest that psychologically stressful situations shift type-1/type-2 cytokine balance toward type-2 and result in an immune dysregulation rather than overall immunosuppression. This may partially explain the increased incidence of type-2-mediated conditions such as increased viral infections, latent viral expression, allergic/asthmatic reactions, and autoimmunity reported during periods of high stress.

    Contributors: Gailen D.Marshall Jr, Sandeep K. Agarwal, Camille Lloyd, Lorenzo Cohen, Evelyn M. Henninger, Gloria J. Morris  
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  • Citation
    Dated: Jun 07, 2008
    Publication Name: J. Microbiol. Biotechnol

    Investigation of the Antifungal Activity and Mechanism of Action of LMWS-Chitosan

    Chitosan, a cationic polysaccharide, has been widely used as a dietary supplement and in a variety of pharmacological and biomedical applications. The antifungal activity and mechanism of action of low molecular weight water-soluble chitosan (LMWS-chitosan) were studied in fungal cells and vesicles containing various compositions of fungal lipids… View more

    Chitosan, a cationic polysaccharide, has been widely used as a dietary supplement and in a variety of pharmacological and biomedical applications. The antifungal activity and mechanism of action of low molecular weight water-soluble chitosan (LMWS-chitosan) were studied in fungal cells and vesicles containing various compositions of fungal lipids. LMWS-chitosan showed strong antifungal activity against various pathogenic yeasts and hyphae-forming fungi but no hemolytic activity or cytotoxicity against mammalian cells. The degree of calcein leakage was assessed on the basis of lipid composition (PC/CH; 10:1, w/w). Our result showing that LMWS-chitosan interacts with liposomes demonstrated that chitosan induces leakage from zwitterionic lipid vesicles. Confocal microscopy revealed that LMWSchitosan was located in the plasma membrane. Finally, scanning electron microscopy revealed that LMWS-chitosan causes significant morphological changes on fungal surfaces. Its potent antibiotic activity suggests that LMWS-chitosan is an excellent candidate as a lead compound for the development of novel anti-infective agents

    Contributors: Park, Yoonkyung, Mi-Hyun Kim, Seong-Cheol Park, Hyeonsook Cheong, Mi-Kyeong Jang, Jae-Woon Nah, and Kyung-Soo Hahm  
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  • Citation
    Dated: Oct 01, 1995
    Publication Name: The Journal of Infectious Diseases

    Drug Cytotoxicity Assay for African Trypanosomes and Leishmania Species

    The trypanosomes and Leishmania species are parasitic protozoa that afflict millions of people throughout the world. If not treated, African trypanosomiasis and visceral leishmaniasis are fatal. The available drugs are severely limited by toxicity, marginal efficacy, the requirement for parenteral administration, and spreading drug resistance. In… View more

    The trypanosomes and Leishmania species are parasitic protozoa that afflict millions of people throughout the world. If not treated, African trypanosomiasis and visceral leishmaniasis are fatal. The available drugs are severely limited by toxicity, marginal efficacy, the requirement for parenteral administration, and spreading drug resistance. In this study, a spectrophotometric assay was developed and validated for measuring the cytotoxicity of test compounds against axenically cultured bloodstream-form Trypanosoma brucei (African trypanosomes) and promastigotes of Leishmania donovani. Enzymatic hydrolysis of p-nitrophenyl phosphate, monitored by a microtiter plate reader, is a reliable surrogate for parasite cell counts. The assay is simple, inexpensive, and highly reproducible. The coefficient of variation for EC50 values is <10% for determinations obtained over several months. This method permits the rapid screening of candidates for much-needed new drugs against these parasites.

    Contributors: Annette L. Bodley, Michael W. McGarry, Theresa A. Shapiro  
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