Absorbance Single-Mode Microplate Readers

Absorbance detection technology

Absorbance single-mode microplate readers can be used for many research applications including ELISA, DNA and protein quantification, and enzyme activity. They are commonly used in a variety of bioresearch and quality control settings.

How does absorbance detection work?
Absorbance single-mode microplate readers measure how much light is absorbed by a sample. Microplate readers that are capable of detecting light in the ultraviolet (UV) range can be used to determine the concentration of nucleic acids (DNA and RNA) or protein directly, without the need for sample labeling. Light of a certain wavelength, dependent on the material being measured, is passed through a sample, and a detector on the other side of the microplate well measures how much of the original light was absorbed by the sample in the well.
How does an absorbance ELISA plate reader work?
An ELISA, or enzyme-linked immunosorbent assay, is a method used to quantitatively detect an antigen within a sample. Most ELISAs are run in microplates, with the bottom of the microplate wells serving as the solid surface to which an antigen of interest attaches, either directly or via an antibody. An enzyme conjugate, which reacts with substrate to produce a colored solution, is used to detect the antigen. A microplate washer is used to wash away non-specific, unbound material in the wells, and a microplate reader―often referred to as an ELISA plate reader―detects the color change produced when target antigen is present.
What's the difference between a spectrophotometer and an absorbance microplate reader?
A standard spectrophotometer measures absorbance one sample at a time. The sample is typically placed in a cuvette through which light is sent horizontally. The SpectrtaMax® QuickDrop Micro-Volume Absorbance Spectrophotometer measures samples as small as 0.5 uL without a cuvette. An absorbance microplate reader offers higher throughput and can measure the absorbance of samples in microplates, typically 96-well or even 384-well, by sending light through each well vertically.

Absorbance measurement applications and research

Find an extensive collection of application notes, research, and technology related to absorbance plate reader assays and applications.

  • Bacterial Identification
  • Bradford, BCA Protein Assay
  • B-galacosidase (Beta GAL)
  • Colorimetric Protein
  • Cytoproliferation
  • Cytotoxicity Assays
  • DNA/RNA/蛋白定量
  • ELISA (HRP, TMB, AP, etc.)
  • Diphenylamine DNA (DISHE)
  • Drug Dissolution Profiles
  • Endpoint ELISAs/EIA
  • Enzyme Kinetics (e.g.,Ki,Km)
  • IC50/LD50
  • 免疫分析
  • Kinetic ELISAs/Enzyme Assays
  • Lowry
  • Microbial Growth/MIC
  • MTT & XTT Cell Viability Assay
More Application notes

SpectraMax ABS/ABS Plus Microplate Readers

快速获得吸光度值,无需借助于滤光片可广泛的应用于各种检测

  • 简洁设计
  • 96- or 384-well microplate compatibility
  • Eight-channel optics for fast reads
  • Pipetting errors eliminated with patented PathCheck® Sensor to measure the optical pathlength of samples in a microplate
  • Validation and compliance tools
了解更多信息

客户研究的重大突破

Université Catholique de Louvain uses our SpectraMax absorbance and multi-mode readers to help fight biofilms.

了解更多信息

准备好了吗?

我们已准备好帮助您解决在研究中遇到的严峻挑战。我们具有遍布全球的经验丰富的专业技术团队,可帮助您实现下一个突破。