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具备样品追踪和孔板处理功能的微生物克隆筛选系统

QPix® 400 系列微生物克隆筛选系统结合了智能成像分析和精准自动化,可对较大的文库进行快速而高效的筛选。每小时能够挑取 3000 个克隆,它将简化您的工作流程。除了微生物筛选外,系统还可自动执行多种样品制备和孔板处理,例如将菌液涂布到琼脂上。通过各种数据追踪和检测工具,QPix 软件可简化对复杂和重复流程的控制和管理。

  • 宽度

    识别特定表型的克隆

    QPix 微生物克隆筛选系统支持各种不同的微生物和多种筛选方式,包括荧光强度、蓝白斑筛选、大小、克隆间距以及抑菌圈。

  • 选择

    高效挑取克隆

    微生物特异性挑针和琼脂厚度探测器可确保高效的挑取。系统具备 >98% 的挑取效率,确保可靠的无看守运行。

  • 无菌

    保持无菌状态

    具有众多灭菌功能,包括用紫外灯对仪器内部消毒、以及挑针的清洗和卤素灯干燥。

特点

  • 微生物特异性挑针

    不同形状和挑取区域的挑针可尽量提高大肠杆菌、噬菌体和酵母菌的挑取效率。涂布专用针可确保将液体培养物均匀分布在琼脂上。

  • 多种成像模式

    可使用白光、荧光和颜色根据预先指定的参数来筛选克隆。滤光片的使用可实现蓝白斑筛选等应用。

  • 涂布和划线

    可在 30 分钟内自动涂布和划线 96 个样品,实现更长时间的无看守运行。

  • 复制、点样和重排

    自动孔板处理和追踪简化了下游检测和样品管理。QPix 微生物克隆筛选系统可提供灵活的孔板复制、重排和点样功能。

  • 琼脂厚度探测器

    超声琼脂厚度探测器可检测因加样量的变化而产生的厚度差,从而提高挑取效率。

  • 可扩展自动化选项*

    QPix HT 型号是一个具有模块化平台并兼容机械臂的解决方案。先进工作流程工程解决方案团队可量身打造支持各种定制服务的微生物克隆筛选系统。

*价格、交付时间和技术参数将根据双方商定的技术要求而有所不同。可能会根据解决方案要求调整标准性能。

QPix 400 系列微生物克隆筛选系统的应用

  • 抗生素抑菌圈

    QPix 软件在抗生素领域的使用

    产抗生素菌株的有效性可以通过细菌板上产生的透明圈大小来测定。QPix 软件让您能够快速识别、排列和挑取产生透明圈的微生物克隆。智能图像分析能够测定板内的透明圈,并根据克隆大小、光晕直径和圆度进行排序。

    生物燃料

    在 QPix 微生物克隆筛选系统中检测生物燃料

    生物柴油是最出色的替代能源资源之一,它是一种由三酰基甘油组成且富含能量的便携式燃料。通过产脂微生物系统来生产生物柴油常需要筛选数千个克隆,分析方法包括通过二喹啉甲酸 (BCA) 检测、光密度测量和气相色谱分析等。QPix 微生物克隆筛选系统可自动执行克隆挑取任务(一个费力且易出错的过程),从而有效缩短找到合适候选克隆的时间。

    阅读应用说明 

  • 蓝白斑筛选

    QPix 400 与蓝白斑筛选

    通过插入克隆化基因来对含有重组质粒的细菌转化株进行筛选是分子克隆中是必不可少的步骤。一种称为“蓝白斑筛选”的比色报告方法可实现根据颜色来轻松识别重组和非重组克隆。QPix 微生物克隆筛选系统可提供一种专门用于使用白光成像来进行蓝白斑比色筛选的自动化解决方案,以高效监测转化效率。其他比色方法,如“红白斑筛选”也可在系统上实施。

    阅读应用说明 

    DNA 测序

    QPix与 DNA 测序

    测序即读取 DNA 分子内腺嘌呤 (A)、鸟嘌呤 (G)、胞嘧啶 (C) 和胸腺嘧啶 (T) 核苷酸的确切顺序。鸟枪法测序是将 DNA 分割成数个含一千个碱基对的片段,然后将其亚克隆到圆形质粒,再转化到细菌中。自动化克隆挑取对于提高测序的通量和质粒纯化至关重要。QPix 微生物克隆筛选系统以可靠性和准确度著称,曾在人类基因组计划中被许多测序中心使用。疫苗开发等许多研究领域中仍在采用传统的测序技术。

    阅读应用说明 

  • 噬菌体展示

    QPix微生物克隆筛选系统与噬菌体展示技术

    噬菌体展示是一种可实现蛋白质、多肽或 DNA 与靶蛋白相互作用研究的技术。这种分子工具可使用噬菌体来呈现病毒外壳表面的靶蛋白,同时融合编码病毒外壳内靶蛋白的 DNA,从而实现高亲和性结合物的发现。可显示结果的噬菌体可被筛选以用于与肽或蛋白文库高通量结合。QPix 微生物克隆筛选系统可在噬菌体展示工作流程中实现自动接种、涂布、划线和挑取。

    了解更多信息 

    蛋白质进化

    微生物克隆筛选系统与蛋白质进化

    蛋白质进化描绘蛋白质形状、功能和组成随时间推移而产生的变化。蛋白质的定向进化经证实是改变和改善大分子活性的一种有效策略,可用于工业、研究和治疗应用。由于具备多个荧光滤光片,该系统可与各种荧光克隆载体兼容。这使 QPix 微生物克隆筛选系统能够在研究蛋白质折叠、酶进化和蛋白定位时显示个别克隆的独特信息。这包括寻找转化标记和筛选突变子。

QPix 400 系列微生物克隆筛选系统的技术参数和可选配置

QPix 400 系列微生物克隆筛选系统的资源

报告内容
视频和网络研讨会
抗原/免疫原发现和优化

免疫学和疫苗开发工作流程

SynBioBeta - QPix 小组讨论2020

SynBioBeta - QPix 小组讨论2020

噬菌斑挑取

噬菌斑挑取

宏基因组系统发育分析

合成宏基因组学:将数字信息转换回生物学

QPix 400

QPix 400

  • Citation
    Dated: Oct 28, 2014
    Publication Name: Front. Microbiol

    Impact of interspecific interactions on antimicrobial activity among soil bacteria

    Certain bacterial species produce antimicrobial compounds only in the presence of a competing species. However, little is known on the frequency of interaction-mediated induction of antibiotic compound production in natural communities of soil bacteria. Here we developed a high-throughput method to screen for the production of antimicrobial… View more

    Certain bacterial species produce antimicrobial compounds only in the presence of a competing species. However, little is known on the frequency of interaction-mediated induction of antibiotic compound production in natural communities of soil bacteria. Here we developed a high-throughput method to screen for the production of antimicrobial activity by monocultures and pair-wise combinations of 146 phylogenetically different bacteria isolated from similar soil habitats. Growth responses of two human pathogenic model organisms, Escherichia coli WA321 and Staphylococcus aureus 533R4, were used to monitor antimicrobial activity. From all isolates, 33% showed antimicrobial activity only in monoculture and 42% showed activity only when tested in interactions. More bacterial isolates were active against S. aureus than against E. coli. The frequency of interaction-mediated induction of antimicrobial activity was 6% (154 interactions out of 2798) indicating that only a limited set of species combinations showed such activity. The screening revealed also interaction-mediated suppression of antimicrobial activity for 22% of all combinations tested. Whereas all patterns of antimicrobial activity (non-induced production, induced production and suppression) were seen for various bacterial classes, interaction-mediated induction of antimicrobial activity was more frequent for combinations of Flavobacteria and alpha- Proteobacteria. The results of our study give a first indication on the frequency of interference competitive interactions in natural soil bacterial communities which may forms a basis for selection of bacterial groups that are promising for the discovery of novel, cryptic antibiotics.

    Contributors: Olaf Tyc, Marlies van den Berg, Saskia Gerards, Johannes A. van Veen, Jos M. Raaijmakers, Wietse de Boer, and Paolina Garbeva  
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  • Citation
    Dated: Mar 19, 2004
    Publication Name: The Journal of Biological Chemistry

    Improved Catalytic Efficiency and Active Site Modification of 1,4-β-D-Glucan Glucohydrolase A from Thermotoga neapolitana by Directed Evolution*

    Thermotoga neapolitana 1,4-β-D-glucan glucohydrolase A preferentially hydrolyzes cello-oligomers, such as cellotetraose, releasing single glucose moieties from the reducing end of the cello-oligosaccharide chain. Using directed evolution techniques of error-prone PCR and mutant library screening, a variant glucan glucohydrolase has been isolated… View more

    Thermotoga neapolitana 1,4-β-D-glucan glucohydrolase A preferentially hydrolyzes cello-oligomers, such as cellotetraose, releasing single glucose moieties from the reducing end of the cello-oligosaccharide chain. Using directed evolution techniques of error-prone PCR and mutant library screening, a variant glucan glucohydrolase has been isolated that hydrolyzes the disaccharide, cellobiose, at a 31% greater rate than its wild type (WT) predecessor. The mutant library, expressed in Escherichia coli, was screened at 85 °C for increased hydrolysis of cellobiose, a native substrate rather than a chromogenic analog, using a continuous, thermostable coupled enzyme assay. The Vmax for the mutant was 108 ± 3 units mg-1, whereas that of the WT was 75 ± 2 units mg-1. The Km for both proteins was nearly the same. The kcat for the new enzyme increased by 31% and its catalytic efficiency (kcat/Km) for cellobiose also rose by 31% as compared with the parent. The nucleotide sequence of two positive clones and two null clones identified 11 single base shifts. The nucleotide transition in the most active clone caused an isoleucine to threonine amino acid substitution at position 170. Structural models for I170T and WT proteins were derived by sequence homology with Protein Data Bank code 1BGA from Paenibacillus polymyxa. Analysis of the WT and I170T model structures indicated that the substitution in the mutant enzyme repositioned the conserved catalytic residue Asn-163 and reconfigured entry to the active site.

    Contributors: James K. McCarthy, Aleksandra Uzelac, Diane F. Davis and Douglas E. Eveleigh  
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  • Citation
    Dated: Aug 21, 2003
    Publication Name: the plant journal

    The transcriptional response of Arabidopsis to genotoxic stress – a high‐density colony array study (HDCA)

    A genome‐wide transcription profiling of Arabidopsis upon genotoxic stress has been performed using a high‐density colony array (HDCA). The array was based on a library of 27 000 cDNA clones derived from Arabidopsis cells challenged with bleomycin plus mitomycin C. The array covers more than 10 000 individual genes (corresponding to at least 40%… View more

    A genome‐wide transcription profiling of Arabidopsis upon genotoxic stress has been performed using a high‐density colony array (HDCA). The array was based on a library of 27 000 cDNA clones derived from Arabidopsis cells challenged with bleomycin plus mitomycin C. The array covers more than 10 000 individual genes (corresponding to at least 40% of Arabidopsis genes). After hybridisation of the HDCA with labelled cDNA probes obtained from genotoxin‐treated (bleomycin plus mitomycin C, 6 h) and untreated seedlings, 39 genes revealed an increased and 24 genes a decreased expression among the 3200 highly expressed clones (representing approximately 1200 individual genes because of redundancy of the cDNA library). Of the 4900 clones with a low transcriptional level, the expression of 500 clones was found to be altered and 57 genes with increased and 22 genes with decreased expression were identified by sequence analysis of 135 identified clones. The HDCA results were validated by real‐time PCR analysis. For about 80% of genes (34 out of 42), alteration in expression was confirmed, indicating the reliability of the HDCA for transcription profiling. DNA damage and stress‐responsive genes encoding, for instance transcription factors (myb protein and WRKY1), the ribonucleotide reductase small subunit (RNR2), thymidine kinase (TK), an AAA‐type ATPase, the small subunit of a DNA polymerase and a calmodulin‐like protein were found to be strongly upregulated. Also, several genes involved in cell cycle regulation revealed significant alteration in transcription, as detected by real‐time PCR analysis, suggesting disturbance of cell cycle progression by mutagen treatment.

    Contributors: I‐Peng Chen, Urs Haehnel, Lothar Altschmied, Ingo Schubert, Holger Puchta  
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