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亲眼见证单克隆性

CloneSelect™ Single-Cell Printer™ 系列采用类似喷墨打印机以及一次性分配分离槽,温和高效地接种单细胞。使用明场高分辨率成像或可选荧光分选细胞,每个单细胞分离捕获 5 张图像。证明单克隆性,提高工作效率,保持并增强细胞活率,且防止交叉污染。

  • 图像

    采集单细胞分离的证据

    在接种细胞时记录 5 张连续图像,以 96 或 384 孔板的形式提供直接的单克隆性图像证据。

  • 高效

    提高克隆形成率

    与传统方法相比,在克隆形成率上可实现高达8倍的提升。高效地将单细胞接种至 96 或 384 孔板中。接种一块96孔板只需约  5 分钟。

  • 选择

    保持细胞健康和无菌

    正如克隆生长实验所见,通过温和的分离维持细胞活性,并使用无菌的一次性单向分离槽防止交叉污染。

CloneSelect 高通量单细胞分离系统

特点

  • 图像-Gn

    图像证据

    每次分配单细胞时可捕获 5 张图像,从而提供可用于监管文件中的单克隆性图像证据。

  • 图像-Gn

    荧光成像和分选

    根据活细胞荧光染料分选活细胞,富集细胞群以高效率地产生荧光报告基因,或分离所需的荧光 CRISPR 克隆。

  • 准确性-Gn

    微流控技术

    无菌分离槽内含专有的硅微流控芯片,可生成包裹细胞的皮升液滴。仅将目标液滴接种到孔板中。

  • 无菌-Gn

    非标记成像和分选

    根据异质细胞群的大小和形态分选细胞,每个孔可选择接种 1 至 100 个细胞。

  • 兼容一系列的细胞

    可分配各种各样的细胞类型,如 CHO、HEK293、L929、T 细胞和 B 细胞。细胞大小从 5 到 40um 不等。

  • 无菌分离槽

    单独包装的无菌分离槽旨在让细胞样本不与仪器接触。一个分离槽可用于制备多块微孔板。

CloneSelect Single-Cell Printer 系列的应用

  • 细胞株开发

    针对重组蛋白的细胞株株开发

    细胞株开发是制备生物制药分子(如单克隆抗体)过程中的关键步骤。该过程通常从将编码目标蛋白的 DNA 转染至宿主细胞开始,目标 DNA 会随机或定向整合至宿主细胞基因组中。筛选数以千计的克隆以鉴别稀有的高产细胞是一个手动且耗时的过程。

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    单克隆性

    细胞株开发和单克隆性验证

    细胞株开发和单克隆性验证是生产生物制药分子(如单克隆抗体)的过程中的关键步骤。在分离稳定表达目标蛋白的单个活细胞后,可建立细胞株。此过程中的一个关键结点是记录单克隆性证据。单克隆性证据通常都是图像形式,据此可生成单细胞图像并将其随附在监管文件中。

    了解更多信息 

  • 单细胞基因组学

    单细胞基因组学

    单细胞分离仍然是单细胞基因组学中一项有挑战性的任务。现行方法缺少证据证明分析容器中仅有一个细胞。细胞在裂解前保持其完整性很重要,以保护其 DNA。

    此外,细胞在裂解前,应受到尽可能少的应激,以保护其 RNA 及其表达水平。CloneSelect Single-Cell Printer 系列可非常温和地分离细胞,从而确保高纯度和高活性。

    这为下游单细胞基因组分析提供了最佳基础。

    单细胞分选

    使用 CloneSelect 单细胞分离系统进行单细胞分选

    细胞株开发要求发现源于单细胞的克隆,它可以高水平地一致地表达目标治疗蛋白。此过程的第一步是分离活的单细胞。有限稀释是一种依赖于统计概率的技术,但很耗时。CloneSelect Single-Cell Printer 能够以尽量提高细胞活性的方式来温和分离单细胞,同时通过在每次单细胞分离时捕获的 5 张连续图像提供单克隆性的直接证据。

CloneSelect Single-Cell Printer 系列的技术参数和可选配置

 

** 用户使用最佳参数进行快速分选时,在明场中可于 <10 分钟内以及在荧光分选(仅 f.sight)中可于 <5 分钟内处理一块含 10 μm 珠悬浮液(溶于无菌滤过水中,浓度约为 5 x 105 至 1 x 106 个珠/ml)的细胞培养 96 孔板。

CloneSelect Single-Cell Printer 系列的资源

报告内容
视频和网络研讨会
开发和建立稳定细胞系

稳定的细胞系开发工作流程

抗原/免疫原发现和优化

免疫学和疫苗开发工作流程

细胞系开发工作流程

细胞株开发的多种不同工作流程

f.Sight 与 FACS 和 LD 概述

f.Sight 与 FACS 和 LD 概述

迅速识别中和抗体

优化的工作流程可用于迅速区分中和抗体与病毒颗粒

CloneSelect 高通量单细胞分离系统

基于微流控技术的单细胞

基于微流控技术的单细胞分离工作流程,已针对效率、存活率和单克隆性验证进行优化

  • Citation
    Dated: Nov 19, 2019
    Publication Name: Biotechnology

    Assuring Clonality on the Beacon Digital Cell Line Development Platform

    During biomanufacturing cell lines development, the generation and screening for single‐cell derived subclones using methods that enable assurance of clonal derivation can be resource‐ and time‐intensive. High‐throughput miniaturization, automation, and analytic strategies are often employed to reduce such bottlenecks. The Beacon platform from… View more

    During biomanufacturing cell lines development, the generation and screening for single‐cell derived subclones using methods that enable assurance of clonal derivation can be resource‐ and time‐intensive. High‐throughput miniaturization, automation, and analytic strategies are often employed to reduce such bottlenecks. The Beacon platform from Berkeley Lights offers a strategy to eliminate these limitations through culturing, manipulating, and characterizing cells on custom nanofluidic chips via software‐controlled operations. However, explicit demonstration of this technology to provide high assurance of a single cell progenitor has not been reported. Here, a methodology that utilizes the Beacon instrument to ensure high levels of clonality is described. It is demonstrated that the Beacon platform can efficiently generate production cell lines with a superior clonality data package, detailed tracking, and minimal resources. A stringent in‐process quality control strategy is established to enable rapid verification of clonal origin, and the workflow is validated using representative Chinese hamster ovary‐derived cell lines stably expressing either green or red fluorescence protein. Under these conditions, a >99% assurance of clonal origin is achieved, which is comparable to existing imaging‐coupled fluorescence‐activated cell sorting seeding methods.

    Contributors: Kim Le, Christopher Tan, Huong Le, Jasmine Tat, Ewelina Zasadzinska, Jonathan Diep, Ryan Zastrow, Chun Chen, Jennitte Stevens  
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  • Citation
    Dated: Jun 28, 2018
    Publication Name: Genetic Engineering & Biotechnology News

    Single-Cell Cloning Remains a Challenge

    Regulatory agencies say that recombinant proteins intended for therapeutic must be produced from a cell line derived from a single progenitor cell. In addition, single-cell cloning has gained increasing importance as genome editing techniques has entered routine laboratory practice. View more

    Regulatory agencies say that recombinant proteins intended for therapeutic must be produced from a cell line derived from a single progenitor cell. In addition, single-cell cloning has gained increasing importance as genome editing techniques has entered routine laboratory practice.

    Contributors: Patricia Fitzpatrick Dimond  
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  • Citation
    Dated: Oct 28, 2017
    Publication Name: Genetic Engineering & Biotechnology News

    Single-Cell Printing for Establishing Monoclonality

    The increasing adoption of large protein biologics such as monoclonal antibodies(mAbs) for the treatment of disease has heralded unprecedented growth in the R&D of novel therapeutics and biosimilar, bringing unique challenges to the biopharmaceuticals company. View more

    The increasing adoption of large protein biologics such as monoclonal antibodies(mAbs) for the treatment of disease has heralded unprecedented growth in the R&D of novel therapeutics and biosimilar, bringing unique challenges to the biopharmaceuticals company.

    Contributors: Sarmad Al-Bassam, Steve Wiltgen  
    Go to article

CloneSelect 高通量单细胞分离系统

 

Molecular Devices 在北美、中国、中国香港、中国澳门和中国台湾提供。对于所有其他国家/地区,请直接联系 Cyten info@cytena.com

 

产品 货号
CloneSelect Single-Cell Printer f.sight 仪器 CLONESELECT-F.SIGHT
CloneSelect Single-Cell Printer c.sight 仪器 CLONESELECT-C.SIGHT
CloneSelect Single-Cell Printer SCP 仪器 CLONESELECT-SCP

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