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用于验证单克隆性的非标记成像解决方案

CloneSelect™ Imager 细胞生长分析系统是一个高通量自动解决方案,可用于哺乳动物细胞的成像和分析。通过多个时间点的成像跟踪可轻松地回溯源自单个细胞的克隆形成过程。自动采集和分析可提供准确、客观和一致的结果。详细的成像和综合数据分析可确保作出更好的决策。凭借在 90 秒内对 96 孔板进行成像的能力,该细胞生长分析系统将提高通量和效率。

  • 验证

    轻松验证单克隆性

    单克隆性报告功能可简化提交至监管机构的支持性材料的准备工作。它将根据您选择的参数自动生成报告。

  • 准确度

    精准检测细胞

    优化算法以精确地识别细胞,并能处理不同的细胞类型和条件。高分辨率成像提供了单克隆性的准确度和可信度。

  • 高效

    在更短时间内筛选更多的克隆

    这款细胞生长分析系统提供业内一流的成像时间,可在 90 秒内对 96 孔板进行成像。

特点

  • 成像模式选择

    非标记明场可实现在不使用有害荧光染料的情况下快速采集图像。自定义荧光成像可增加单克隆性的可信度。

  • 自动生成的数据和工具

    该软件自动计算汇合度并生成生长曲线、热图和拼接图像。可随时间推移自动追踪每个孔的测定值。

  • 各种孔板类型和细胞类型

    此细胞生长分析系统兼容 6 至 384 孔板且可用于悬浮细胞和贴壁细胞,如 CHO、HEK、杂交瘤细胞、Per.C6®®、Jurkat、SupT1、H-4-II-E、MCF-7、JT29、DLD1 和 KB31。

  • 智能分析

    该软件自动计算每个成像时间点的汇合度。自动生成可导出的生长曲线、拼接图像、总生长率和平均速率。

  • 清晰简洁的图像

    此细胞生长分析系统拥有可自动对焦的 4x 物镜。高分辨率 CCD 相机成像达到每像素 1.85 微米,即使在孔边缘等难以成像的区域也能实现精准的细胞检测。

  • 定制自动化选项*

    先进工作流程工程解决方案团队提供各种定制服务,涵盖从机械臂孔板加载到整合液体工作站和培养箱的全自动工作站等各个方面。

*价格、交付时间和技术参数将根据双方商定的技术要求而有所不同。可能会根据解决方案要求调整标准性能。

CloneSelect Imager 细胞生长分析系统的应用

CloneSelect Imager 细胞生长分析系统的技术参数和可选配置

CloneSelect Imager 的资源

报告内容
视频和网络研讨会
开发和建立稳定细胞系

稳定的细胞系开发工作流程

杂交瘤工作流程

杂交瘤工作流程

细胞系开发工作流程

细胞株开发的多种不同工作流程

迅速识别中和抗体

优化的工作流程可用于迅速区分中和抗体与病毒颗粒

CloneSelect Imager 细胞生长分析系统视频

CloneSelect Imager 细胞生长分析系统

G 蛋白偶联受体细胞系选择

使用 ClonePix 2 来识别和选择 GPCR 细胞系

  • Citation
    Dated: Jan 12, 2021
    Publication Name: Merck Research Laboratory

    A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

    A high-throughput, automated platform of manufacturing cell line development for producing protein therapeutics is described. Implementation of BD FACS Aria Cell Sorter, CloneSelect Imager and TECAN Freedom EVO liquid handling system has demonstrated significantly increased processing capacity in cell line development with improved cell line… View more

    A high-throughput, automated platform of manufacturing cell line development for producing protein therapeutics is described. Implementation of BD FACS Aria Cell Sorter, CloneSelect Imager and TECAN Freedom EVO liquid handling system has demonstrated significantly increased processing capacity in cell line development with improved cell line quality and high reproducibility.

    Contributors: Shuangping Shi, Russ G.G. Condon, Liang Deng, Jason Saunders, Finn Hung, Yung-Shyeng Tsao, Zhong Liu  
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  • Citation
    Dated: Jan 12, 2021
    Publication Name: Molecular Devices

    Rapid Monoclonality Verification Methods to Boost Cell Line Development

    Limiting dilution or fluorescence activated cell sorting is typically performed to seed single cells into a well. Microscopy is then used to determine the number of cells seeded in each well and monitor cell growth. While monoclonality verification via white light imaging is possible, debris, dust, and air bubbles make it difficult and time… View more

    Limiting dilution or fluorescence activated cell sorting is typically performed to seed single cells into a well. Microscopy is then used to determine the number of cells seeded in each well and monitor cell growth. While monoclonality verification via white light imaging is possible, debris, dust, and air bubbles make it difficult and time consuming to identify single cells which may cause high value clones to be discarded. Here we present a fluorescent method for identifying monoclonal CHO-S cells using CellTracker Green CMDFA. We incubated CHO-S cells with Cell Tracker Green CMDFA and performed limited dilution to seed single CHO-S cells into 96-well plates. The CloneSelect Imager was used to image CHO-S cells in white light and fluorescence channels. By using fluorescence to identify cells, monoclonality verification is easier and more conclusive than using white light imaging or microscopy alone.

    Contributors: Wilson Lew, Anna Forsyth, John Philips, Alison Glaser, Natalia Lysaya  
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  • Citation
    Dated: Jun 17, 2010
    Publication Name: BMC Biotechnology

    Accurate non-invasive image-based cytotoxicity assays for cultured cells

    High-throughput screening methods for toxicology evaluation in the early phases of drug discovery are highly desired. In the early 1980´s a novel assay for cell survival determination was reported [1], which has been frequently used to study the biological activity of a variety of potential cytostatic drugs. The assay was presented as a rapid,… View more

    High-throughput screening methods for toxicology evaluation in the early phases of drug discovery are highly desired. In the early 1980´s a novel assay for cell survival determination was reported [1], which has been frequently used to study the biological activity of a variety of potential cytostatic drugs. The assay was presented as a rapid, precise and simple method to detect living cells in mammalian cell cultures, using the tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and a microtiter plate reader.

    Contributors: Patricia Marqués-Gallego, Hans den Dulk, Claude Backendorf, Jaap Brouwer, Jan Reedijk & Julian F Burke  
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