High throughput Octet HTX and Octet RED384 Systems
Perform your quantitation and kinetics characterization assays at unmatched speed and throughput
Octet HTX offers unprecedented speed - antibody quantitation, kinetics, epitope binning, and much more
From IgG titer to epitope binning, the Octet HTX and Octet RED384 Systems offer label-free molecular interaction analysts with state-of-the-art high throughput platforms that facilitate the rapid characterization and development of biologics drug molecules.
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Analyze up to 96 samples simultaneously
Perform your quantitation or kinetics experiments with unmatched speed. Quantitate up to 96 samples of an IgG sample in two minutes.
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Perform a 32 x 32 epitope binning in less than eight hours
Use in-tandem, sandwich, or pre-mix assay designs to perform epitope binning. Complete a 32 x 32 epitope binning in less than eight hours.
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Streamline workflows with automation
The Octet HTX and Octet RED384 are automation ready and are robot compatible for microplate and biosensor tray loading.
Automated workflow solutions for the Octet HTX and RED384 systems
Features
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Microfluidics-free Dip and Read format reduces assay time and maintenance cost
The Octet HTX and Octet RED384 utilize ForteBio's Dip and Read™ technology with the read head dipping into samples in a plate format. With no fluidics involved, Octet systems are highly robust.
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Simultaneously reads 8, 16, 32, 48 or 96 wells
The HTX read head provides design flexibility that allows users to analyze, 8, 16, 32, 48, or 96 samples at a time allowing for customized through put up to 96 samples.
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Full plate off-rate ranking in minutes, not hours
The Dip and Read assay format allows off-rate analysis of clones to be performed rapidly even in crude samples using ready-to-use biosensors off the shelf.
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96-well plate quantitation in as little as two minutes
IgG titer determination of a full 96-well plate can be completed in two minutes. In addition, sample plate compartment is compatible with 384-well plates allowing for even more throughput.
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32 x 32 epitope binning in less than eight hours
With the HTX, analysis of epitope binning matrices can be completed in record time, with 32 x 32 in less than eight hours. A powerful epitope binning analysis software enables easy visualization of data sets and provides a flexible data matrix that can accommodate a variety of cross-blocking formats.
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Application Note
A rapid method to quantitatively screen bispecific antibody using Protein A and His1K biosensors
A rapid method to quantitatively screen bispecific antibody using Protein A and His1K biosensors
The production of bsAbs adds a challenge for scientists in the cell line screening process since many other conformations of antibodies can be produced by the cells.
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Application Note
Reducing Variability in Small Molecule Screening and Kinetics Applications
Reducing Variability in Small Molecule Screening and Kinetics Applications
Minimizing the variability of background signals is a key parameter to the success of demanding applications such as small molecule analysis1. By reducing the background variability,…
Applications for High Throughput Octet Systems
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ELISA Conversion
Despite its widespread adoption in industry, variability in results, susceptibility to human error, labor intensive workflow, and slow times-to-result remain as big hurdles for users looking to adopt ELISA in high throughput applications. High throughput Octet systems are aptly designed to solve these problems. ELISA assays can be directly transferred onto Octet systems.
ELISA Conversion
Despite its widespread adoption in industry, variability in results, susceptibility to human error, labor intensive workflow, and slow times-to-result remain as big hurdles for users looking to adopt ELISA in high throughput applications. High throughput Octet systems are aptly designed to solve these problems. ELISA assays can be directly transferred onto Octet systems.
Epitope Binning
Epitope binning studies rely on the sequential binding of two antibodies to an antigen, and are performed using dozens of antibody pairs in cross-competition matrices. The Octet system excels at these large-scale studies due to assay speed, throughput, and exceptional reproducibility
Epitope Binning
Epitope binning studies rely on the sequential binding of two antibodies to an antigen, and are performed using dozens of antibody pairs in cross-competition matrices. The Octet system excels at these large-scale studies due to assay speed, throughput, and exceptional reproducibility
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Protein Contaminant Testing
Protein contaminants are a major hurdle to overcome during the production of biopharmaceuticals. The Octet® platform's rapid high throughput protein analysis combined with the anti-CHO HCP and Residual Protein A kits (designed with sensitivity in mind) provide biologics developers with a rapid and easy to use technique for contaminant testing
Protein Contaminant Testing
Protein contaminants are a major hurdle to overcome during the production of biopharmaceuticals. The Octet® platform's rapid high throughput protein analysis combined with the anti-CHO HCP and Residual Protein A kits (designed with sensitivity in mind) provide biologics developers with a rapid and easy to use technique for contaminant testing
Specification & Options of High Throughput Octet Systems: Octet HTX and Octet RED384
Resources of High Throughput Octet Systems: Octet HTX and Octet RED384
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Application Note
A rapid method to quantitatively screen bispecific antibody using Protein A and His1K biosensors
A rapid method to quantitatively screen bispecific antibody using Protein A and His1K biosensors
The production of bsAbs adds a challenge for scientists in the cell line screening process since many other conformations of antibodies can be produced by the cells.
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Application Note
Reducing Variability in Small Molecule Screening and Kinetics Applications
Reducing Variability in Small Molecule Screening and Kinetics Applications
Minimizing the variability of background signals is a key parameter to the success of demanding applications such as small molecule analysis1. By reducing the background variability,…
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Application Note
CaptureSelect affinity ligands for antibody detection and characterization
CaptureSelect affinity ligands for antibody detection and characterization
BAC’s CaptureSelect affinity ligands provide a unique group of molecules that can be designed for almost any purification target, with as narrow or as broad specificity as is required…
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Application Note
Rapid, reliable quantitation of Fc-Fusion protein in cell culture supernatants
Rapid, reliable quantitation of Fc-Fusion protein in cell culture supernatants
The cell line development group at Biogen IDEC needed a robust assay for the measurement of Fc-fusion protein (Protein 1) in crude cell culture supernatants. The group had historically…
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Application Note
Cell-Based Assay Using Biosensor-captured Red Blood Cells
Cell-Based Assay Using Biosensor-captured Red Blood Cells
Cell-based assays that qualitatively measure the function of an interacting molecule under physiological conditions provide valuable information for drug discovery. Two approaches are…
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Application Note
Customized quantitation of recombinant therapeutic proteins using High Precision Streptavidin biosensors (SAX)
Customized quantitation of recombinant therapeutic proteins using High Precision Streptavidin biosensors (SAX)
The accurate determination of recombinant protein titer is critical to the selection of high-producing clones during cell line development and in optimization of bioreactor conditions…
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Application Note
Optimizing protein-protein and protein-small molecule kinetics assays
Optimizing protein-protein and protein-small molecule kinetics assays
Kinetic analysis of protein-protein and protein-small molecule interactions is a key application for real-time, label-free systems such as the Octet® instrument family. The Octet…
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Application Note
MAb Quantitation: Protein A HPLC vs. Protein A Bio-layer Interferometry
MAb Quantitation: Protein A HPLC vs. Protein A Bio-layer Interferometry
There are currently 30 monoclonal antibodies approved by the FDA as biotherapeutic agents, representing the most rapidly growing class of new drugs. Despite advances in downstream…
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Application Note
Robust Quantitation Assay for Monoclonal Antibodies and Other Proteins on Octet Platform
Robust Quantitation Assay for Monoclonal Antibodies and Other Proteins on Octet Platform
The Octet platform provides analytical methods for measuring antibody and protein concentrations and binding affinities through various stages of drug discovery and development (Table…
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Application Note
Cross-competition or Epitope Binning Assays on the Octet HTX System
Cross-competition or Epitope Binning Assays on the Octet HTX System
Epitope binning is a term used to describe segmentation of a panel of monoclonal antibodies (mAbs) into “bins” based upon the antigen region, or epitope, bound by each antibody. This…
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Application Note
Converting an ELISA Assay into an Octet Quantitation Assay
Converting an ELISA Assay into an Octet Quantitation Assay
Quantitation assays on the Octet platform have many similari- ties to enzyme-linked immunosorbent assays (ELISA). Both are performed on a solid support on which the capture molecule is…
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Application Note
Instant Determination of Protein Presence Using the BLItz System
Instant Determination of Protein Presence Using the BLItz System
The BLItz system provides a simple, rapid Dip and Read™ approach to protein analysis in an affordably priced personal assay system. Protein and antibody detection can be performed in a…
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Application Note
A Fast and High Precision Influenza Vaccine Potency Assay
A Fast and High Precision Influenza Vaccine Potency Assay
Vaccines are biological preparations that contain agents resem- bling disease causing microorganisms, and can improve immu- nity against a specific disease. They are typically prepared…
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Application Note
Generating Reliable Kinetic Data for Protein-Ligand Interactions
Generating Reliable Kinetic Data for Protein-Ligand Interactions
The Community Structure Activity Resource (CSAR, www.csardock. org) group is developing a database of high quality protein-ligand structures and the corresponding binding affinities.…
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Application Note
Kinetic Analysis of Antibody Binding to an Expressed Membrane Protein on Captured Lipoparticles
Kinetic Analysis of Antibody Binding to an Expressed Membrane Protein on Captured Lipoparticles
Membrane proteins govern the majority of input and output signals of cells and represent the largest class of pharmaceutical drug targets, making the analysis of their molecular…
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Application Note
Analysis of FcRn-Antibody Interactions on the Octet Platform
Analysis of FcRn-Antibody Interactions on the Octet Platform
The Fc region of human IgG contributes to a number of beneficial biological and pharmacological characteristics of therapeutic antibodies. One of the most important is prolonging…
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Application Note
Biomolecular Binding Kinetics Assays on the Octet Platform
Biomolecular Binding Kinetics Assays on the Octet Platform
Direct measurement of biomolecular interactions plays an important role in biotherapeutic drug discovery and development. Label-Free analytical technologies such as the Octet® platform…
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Application Note
Analysis of Fc-gamma Receptor-IgG Interactions on the Octet Platform
Analysis of Fc-gamma Receptor-IgG Interactions on the Octet Platform
Fc gamma receptors (FcγRs) are membrane glycoproteins with affinity for the Fc region of immunoglobulin G (IgG). FcγRs expressed on the surface of immune effector cells play a key role…
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Application Note
Validated Quantitation and Activity Assay of Antibody Fragment Molecule (Fab) for Process Development and Quality Control
Validated Quantitation and Activity Assay of Antibody Fragment Molecule (Fab) for Process Development and Quality Control
The group was able to develop a working Fab activity assay on the Octet RED system in less than a week. Relative to the overnight incubation and four-hour assay time of their ELISA…
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Application Note
Octet Potency Assay: Development, Qualification and Validation Strategies
Octet Potency Assay: Development, Qualification and Validation Strategies
Kinetic analysis of biomolecular interactions is critical during drug discovery and development. The affinity of an interaction directly affects the dose required for a…
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Application Note
Use of the Octet QK System to Enhance Service in a Core Hybridoma Laboratory
Use of the Octet QK System to Enhance Service in a Core Hybridoma Laboratory
The Hybridoma Laboratory is a part of the University of Florida Interdisciplinary Center for Biotechnology Research. The main service provided by this laboratory is development of new…
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Application Note
Enhancing Efficiency and Economics in Process Development and Manufacturing of Biotherapeutics
Enhancing Efficiency and Economics in Process Development and Manufacturing of Biotherapeutics
Analytical techniques that measure protein quantity and quality are used in nearly all stages of research, process development and manufacturing of biotherapeutics. UV spectroscopy,…
Data Sheet
Ready BLI Detection Kit – Residual Protein A
Ready BLI Detection Kit – Residual Protein A
Detecting and minimizing Protein A contamination is a critical quality control step in antibody therapeutic development and production. The Ready BLI Detection kit - Residual Protein A…
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Data Sheet
Octet Biosensors Quantitation Grade
Octet Biosensors Quantitation Grade
To order biosensor consumables, a purchase order referencing a quote number should be faxed or emailed to the following:
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Data Sheet
Instrument Qualification (IQ)/Operational Qualification (OQ) Documentation Manual
Instrument Qualification (IQ)/Operational Qualification (OQ) Documentation Manual
The Octet Installation Qualification (IQ) and Operational Qualification (OQ) manual provides the procedures to document and verify that the correct Octet System was ordered, delivered a…
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Data Sheet
Compliance Validation Software Module
Compliance Validation Software Module
ForteBio’s Compliance Validation Module for the Octet System provides the tools to comply with the FDA guidelines for 21 CFR part 11. Key features include security, data validation and…
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Data Sheet
Streptavidin High Binding FA Biosensors (Screening Grade)
Streptavidin High Binding FA Biosensors (Screening Grade)
ForteBio Streptavidin High Binding FA Biosensors, in conjunction with the Octet System, are designed for immobilization of biotin labeled proteins for use in assaying protein:protein in…
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Data Sheet
Octet System Overview: Label-Free, Real-Time Analysis for Bioprocessing
Octet System Overview: Label-Free, Real-Time Analysis for Bioprocessing
The FortéBio Octet System has been designed to address the need to accelerate the production and characterization of antibody and protein therapeutics in a simple label-free format. By…
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Data Sheet
References to Fortebio’s Products in Published Literature
References to Fortebio’s Products in Published Literature
A Global Benchmark Study Using Affinity-based Biosensors. Rich, R.L. and others, Analytical Biochemistry, 2009, 386 (2), 194-216.
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Data Sheet
Octet® Instrument Workstation
Octet® Instrument Workstation
Pall ForteBio Octet instruments use Microsoft Windows-based computers to control instrument functions and to collect and analyze your experiment data, with Microsoft Office functions ge…
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Data Sheet
Host Cell Protein Assay Development Services
Host Cell Protein Assay Development Services
ForteBio offers a range of high-quality, custom assay development services to help customers develop specific assays for its Octet platform of instruments, including host cell protein (…
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Data Sheet
Octet System Label-free, real-time detection of molecular interactions
Octet System Label-free, real-time detection of molecular interactions
ForteBio’s Octet systems incorporate a dedicated instrument for reading proprietary Bio-Layer Interferometry (BLI) plus a complete range of ready-to-use biosensors for real-time, label-…
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Data Sheet
ForteBio Octet System
ForteBio Octet System
ForteBio’s Octet systems incorporate a dedicated instrument for reading proprietary Bio-Layer Interferometry (BLI) using a complete range of ForteBio’s ready-to-use biosensors for real-…
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Data Sheet
Important Product Change Notification
Important Product Change Notification
ForteBio will be switching to Kathon as the preservative in Sample Diluent, Kinetics Buffer 10X and IgG Calibrators. As stated in the above table – HCP assay kits, Amine Coupling reagen…
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Data Sheet
Octet Annual Service Contracts
Octet Annual Service Contracts
Octet® service contracts are not just for repair services. They help keep your Octet system in top condition, and provide you priority access to our global network of Technical Support,…
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Data Sheet
Octet Software v7.0 for Data Acquisition and Data Analysis
Octet Software v7.0 for Data Acquisition and Data Analysis
ForteBio’s Octet software provides an intuitive and easy to use interface for data acquisition and analysis on all Octet instruments, enabling label-free kinetic, affinity, concentratio…
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Data Sheet
Protein A Biosensors
Protein A Biosensors
Optical glass Biosensors (coated with Protein A), fixed in molded plastic hubs, stored 96 each in molded plastic trays.
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Data Sheet
MSDS Sample Diluent
MSDS Sample Diluent
Phosphate Buffered Saline, pH 7.4, with 0.1% Bovine Serum Albumin and Tween 20 detergent (0.02%). Sodium Azide (0.05%) used as preservative.
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Data Sheet
Human IgG Calibrators
Human IgG Calibrators
Purified Human IgG in Phosphate Buffered Saline, pH 7.4, with 0.1% Bovine Serum Albumin, Tween 20 detergent (0.02%) and protease inhibitors. Sodium Azide (0.05%) used as preservative.
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Data Sheet
Octet Software non CRF Comparison
Octet Software non CRF Comparison
Pall ForteBio Octet Data Acquisition and Data Analysis software is rapidly evolving to better address the growing applications and needs of our customers. The table below provides a gen…
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Data Sheet
Octet Software CRF Comparison
Octet Software CRF Comparison
Octet CFR software and our GxP Server enable data acquisition and data analysis in laboratories working under GMP, GLP, and 21 CFR Part 11 regulations. It also provides all necessary te…
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Data Sheet
Octet Software Version 10.0: Data Acquisition, Data Analysis and Data Analysis High Throughput (HT)
Octet Software Version 10.0: Data Acquisition, Data Analysis and Data Analysis High Throughput (HT)
Octet® software provides an intuitive and easy to use interface for data acquisition and analysis on all Octet instruments, enabling label-free kinetic, affinity, epitope binning, activ…
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Data Sheet
Anti-human IgG (AHQ) Biosensors (Fc-specific)
Anti-human IgG (AHQ) Biosensors (Fc-specific)
Direct measurement of immunoglobulins (IgG) Assay samples without centrifugation Fast turnaround of results Correlates to HPLC ForteBio’s Dip and Read™ Anti-Human IgG (AHQ) biosensors,…
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Data Sheet
Streptavidin (SA) Biosensors
Streptavidin (SA) Biosensors
ForteBio Streptavidin (SA) biosensors are designed for immobilization of biotin labeled proteins for use in assaying protein:protein interactions using the Octet® platform. The systems…
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Data Sheet
Protein L Biosensors
Protein L Biosensors
Dip and Read™ Protein L (ProL) biosensors provide a rapid and direct method for quantifying a broad set of kappa light chain containing immunoglobulins, including whole molecules, FAb f…
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Data Sheet
Anti-Mouse IgG Fc Capture (AMC) Biosensors
Anti-Mouse IgG Fc Capture (AMC) Biosensors
Dip and Read™ Anti-Mouse IgG Fc Capture (AMC) biosensors enable kinetic characterization of macromolecular interactions between mouse Fc-containing proteins and target analytes. Immobil…
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Data Sheet
Amine Reactive Second-Generation Biosensors
Amine Reactive Second-Generation Biosensors
The Dip and Read™ Amine Reactive 2nd-Generation (AR2G) biosensors enable kinetic characterization of macromolecular interactions between purified proteins and target analytes. Immobiliz…
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Data Sheet
Anti-GST Biosensors
Anti-GST Biosensors
Glutathione-S-transferase (GST) is commonly fused to recombinant proteins to facilitate detection and purification, and increase solubility. The robust properties of the GST tag have es…
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Data Sheet
High Precision Streptavidin (SAX) Biosensors
High Precision Streptavidin (SAX) Biosensors
Streptavidin-coated surfaces are widely used as a simple and straightforward method of molecular immobilization. Utilized with Bio-Layer Interferometry (BLI), Streptavidin biosensors en…
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Data Sheet
Anti-Penta-HIS (HIS1K) Biosensors
Anti-Penta-HIS (HIS1K) Biosensors
The polyhistidine tag, commonly known as His-tag, is fused to recombinant proteins as a means of facilitating detection and purification. The Dip and Read™ Anti-Penta-HIS (HIS1K) Biosen…
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Data Sheet
Octet Software Version 9.0: Data Acquisition, Data Analysis and Data Analysis High Throughput (HT)
Octet Software Version 9.0: Data Acquisition, Data Analysis and Data Analysis High Throughput (HT)
Octet® software provides an intuitive and easy to use interface for data acquisition and analysis on all Octet instruments, enabling label-free kinetic, affinity, epitope binning, activ…
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Data Sheet
Anti-Human Fab-CH1 2nd Generation Biosensors
Anti-Human Fab-CH1 2nd Generation Biosensors
Human antibodies are the most vital research candidates in drug discovery and development of bio-therapeutics. The detection and characterization of human IgG is of paramount importance…
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Data Sheet
Anti-human IgG Fc Capture (AHC) Biosensors
Anti-human IgG Fc Capture (AHC) Biosensors
Capture-based immobilization of human Fc-containing proteins Regenerable and cost-effective format Compatible with crude samples and complex media The Dip and Read™ Anti-Human IgG Fc Ca…
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Data Sheet
Octet software comparison
Octet software comparison
ForteBio Octet Data Acquisition and Data Analysis software is rapidly evolving to better address the growing applications and needs of our customers. These comparison tables provide a g…
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Data Sheet
Octet HTX System
Octet HTX System
The Octet ® HTX instrument monitors up to 96 biosensors simultaneously, enabling label-free detection for protein quantitation and kinetic characterization at unmatched speed. The syste…
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Data Sheet
Octet RED384 System
Octet RED384 System
ForteBio’s Octet® RED384 system is designed for increased throughput for label-free protein quantitation and kinetic characterization. Get accurate concentration, kinetic constants, and…
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Data Sheet
Octet system qualification products and services
Octet system qualification products and services
Regulations and global standards, including US FDA 21 CFR Part 11, EU GMP Annex 15 and USP <1058>, require documented verification that your instruments are delivered, installed a…
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Data Sheet
Dip and Read™ Anti-CHO HCP Detection Kit
Dip and Read™ Anti-CHO HCP Detection Kit
Host cell proteins (HCPs) are contaminants found in biopharmaceuticals expressed in bacterial, yeast or mammalian production cell lines. Among protein expression cell lines, Chinese ham…
eBook
Label-free detection: technologies, key considerations, and applications
Label-free detection: technologies, key considerations, and applications
Interactions between biomolecules serve as key triggers for many biological processes and, therefore, provide perfect targets for drug discoveries.
Flyer
Cell line development: accelerating antibody discovery by monitoring titer and glycosylation with the Octet platform
Cell line development: accelerating antibody discovery by monitoring titer and glycosylation with the Octet platform
Learn how Cell line development accelerating the screening of antibody discovery by monitoring titer and glycosylation with the Octet platform.
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Flyer
Integrated workflow for rapid selection and optimization of hybridoma cell lines
Integrated workflow for rapid selection and optimization of hybridoma cell lines
Hybridomas are comprised of the fusion between B-cell splenocytes from antigen-immunized mice and a myeloma tumor cell line result in immortalized antibody-producing clonal cell lines t…
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Flyer
Octet System: Label-Free, Real-Time Kinetic Analysis for Bioprocessing
Octet System: Label-Free, Real-Time Kinetic Analysis for Bioprocessing
The kinetic analysis of antibodies is critical to both the selection of clones for development and as a method of monitoring antibody activity throughout therapeutic antibody production…
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Flyer
Octet System: Monitoring Antibody Production
Octet System: Monitoring Antibody Production
Accurate antibody quantitation is critical to the selection of cell lines for development and the optimization of antibody production. FortéBio’s Octet provides rapid and accurate analy…
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Flyer
BLI Biosensors Selection Guide
BLI Biosensors Selection Guide
Read this document which is helpful in the selection of Biosensor with their detail information.
Newsletter
NEW Dip and Read™ Anti-Mouse IgG (Fc) Capture (AMC) Biosensors
NEW Dip and Read™ Anti-Mouse IgG (Fc) Capture (AMC) Biosensors
Monoclonal antibodies are key tools for basic research as well as for diagnosis and treatment of human diseases. Therapeutic monoclonal antibodies approached $50 billion in worldwide sa…
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Newsletter
NEW Protein G Biosensor Available for the Octet Platform!
NEW Protein G Biosensor Available for the Octet Platform!
Immunoglobulin-binding proteins such as Protein A, Protein G and Protein L are commonly used to purify, immobilize or detect immunoglobulins. They have different binding profiles with r…
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Newsletter
NEW Ni-NTA Biosensors for HIS-tagged Biomolecule Analysis
NEW Ni-NTA Biosensors for HIS-tagged Biomolecule Analysis
The robustness of the HIS-tag and its ease of use have established it as one of the most commonly used tags in the biopharmaceutical industry. It’s commonly fused to recombinant protein…
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Newsletter
NEW Anti-FLAG Biosensors for FLAG-tagged Biomolecule Analysis
NEW Anti-FLAG Biosensors for FLAG-tagged Biomolecule Analysis
Continuing on an unprecedented record of technological innovation, ForteBio is excited to announce the launch of our Anti-FLAG Biosensor. The FLAG® polypeptide tag (DYKDDDDK) was one of…
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Newsletter
New Protein L Biosensor For Antibody Fragment Quantitation
New Protein L Biosensor For Antibody Fragment Quantitation
Antibodies are so rich in biochemical features that nearly every piece and parcel of their structure has been harvested and put to use, leading to widespread adoption of whole molecules…
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Newsletter
Residual Protein A Detection Assay
Residual Protein A Detection Assay
Detection of residual Protein A in product purified from a Protein A, or similar purification column, is a critical quality control step in antibody therapeutic development and producti…
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Newsletter
Let the Label-free Revolution Begin! New Octet 384 Instrument Series
Let the Label-free Revolution Begin! New Octet 384 Instrument Series
ForteBio is pleased to announce the launch of two new instruments for label-free, realtime quantitation and kinetic analysis. The Octet QK384 and Octet RED384 16-channel instruments com…
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Newsletter
Anti-human IgG (Fc) Capture Biosensors for Immobilization and Kinetic Characterization of Human IgG and Other Fc-containing Proteins
Anti-human IgG (Fc) Capture Biosensors for Immobilization and Kinetic Characterization of Human IgG and Other Fc-containing Proteins
The Anti-Human IgG Fc Capture (AHC) biosensor is designed for kinetic characterization of macromolecular interactions between proteins containing an Fc region and a target analyte. This…
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Newsletter
Fast and Easy Generic Anti-CHO HCP Analysis: 96 Samples, Assay to Data in 65 Minutes with the NEW Anti-CHO HCP Detection Kit
Fast and Easy Generic Anti-CHO HCP Analysis: 96 Samples, Assay to Data in 65 Minutes with the NEW Anti-CHO HCP Detection Kit
Pall ForteBio LLC and Cygnus Technologies recently teamed up to jointly develop our new Anti-CHO HCP Detection Kit for quantitation of residual HCPs. Cygnus HCP ELISA kits are well know…
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Newsletter
Octet Rapid System—Extended Detection
Octet Rapid System—Extended Detection
ForteBio is proud to announce the introduction of the new Octet RED System, a label-free interaction analysis instrument with extended detection capabilities. The new system is particul…
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Newsletter
The Octet HTX System Raises the Bar on Performance
The Octet HTX System Raises the Bar on Performance
ForteBio is pleased to announce the launch of the Octet HTX system, the first BLI instrument to monitor up to 96 biosensors simultaneously. The Octet HTX system provides the highest lev…
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Newsletter
Second-generation Amine Reactive Biosensor Advances the Octet Platform
Second-generation Amine Reactive Biosensor Advances the Octet Platform
Amine coupling using EDC and NHS or sulfo-NHS is a classic method of coupling a carboxylate to a primary amine to form a covalent amide bond. The chemistry has been widely used in label…
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Newsletter
NEW Octet RED96 System Advances Label-Free Concentration and Kinetic Analysis
NEW Octet RED96 System Advances Label-Free Concentration and Kinetic Analysis
ForteBio announces the launch of the Octet RED96 system to deliver unparalleled performance in the measurement of high quality kinetic, affinity, concentration and specificity interacti…
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Newsletter
Affinity-Tag Mediated Capture for Label-Free Biomolecular Interaction Analysis
Affinity-Tag Mediated Capture for Label-Free Biomolecular Interaction Analysis
In the post-genomic era, most analytical tools for studying the structure and function of recombinant proteins are limited by being specific to each individual protein, its sequence, an…
Brochure
Integrated Solutions for Early Stage Biologics Discovery
Integrated Solutions for Early Stage Biologics Discovery
ForteBio’s mission is to fast track timelines by removing hurdles in early stage biologics development through innovative technologies and workflow solutions.
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Brochure
Integrated Octet Systems
Integrated Octet Systems
Maximize your Octet system’s unattended run time The Octet® HTX and RED384 Systems are the industry-leading platform for monitoring the binding of proteins and other biomolecules direc…
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Brochure
Octet Systems
Octet Systems
Your research should be guided by your imagination, not by the limitations of your label-free system. The Octet® platform’s flexible, open-format design and comprehensive menu of biosen…
Scientific Poster
High throughput sialylation screening on Octet label free-instrument for expediting clone selection process in cell line development
High throughput sialylation screening on Octet label free-instrument for expediting clone selection process in cell line development
Explore more about the high-throughput method for relative screening of terminal sialic acid content was developed on the Octet platform to expedite cell line development.
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Scientific Poster
Octet BLI systems – unmatched versatility for discovery, development and quality control
Octet BLI systems – unmatched versatility for discovery, development and quality control
Traditional methods for analyzing the kinetics of biomolecular interactions such as ELISA are often cumbersome and may require rigorous sample preparation. In addition, ELISA may not pr…
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Scientific Poster
Amine reactive biosensors add flexibility to the Octet System for label free kinetics analysis and rapid screening
Amine reactive biosensors add flexibility to the Octet System for label free kinetics analysis and rapid screening
ForteBio’s Octet System, based on proprietary Bio-Layer Interferometry (BLI), is an established system that enables label-free, real-time detection and analysis of molecular interaction…
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Scientific Poster
Efficient and cost-effective workflow for antibody bioprocessing using ForteBio’s Octet system for label-free, real-time analysis
Efficient and cost-effective workflow for antibody bioprocessing using ForteBio’s Octet system for label-free, real-time analysis
Current analytical methods for antibody bioprocessing require time consuming and technician-dedicated procedures that impact workflow, hands-on time and cost. The Octet - QK generates q…
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Scientific Poster
ForteBio’s Octet - A Novel System for Measuring Protein Interactions in Antibody Development and Production
ForteBio’s Octet - A Novel System for Measuring Protein Interactions in Antibody Development and Production
The effi cient development and production of antibodies requires analytical methods that are robust, accurate and real-time. The methods should also be simple to run, require minimal tr…
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Scientific Poster
Developing label-free, real-time detection of human polyclonal and monoclonal IgG with a Protein A biosensor on ForteBio's Octet
Developing label-free, real-time detection of human polyclonal and monoclonal IgG with a Protein A biosensor on ForteBio's Octet
We report the high-throughput, real-time quantitation of human or chimeric IgG with ForteBio's Octet in a 96-well plate format with a fiber-optic biosensor coated with Protein A.
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Scientific Poster
Development of the ForteBio™ Octet System for label-free, real-time analysis of molecular interactions
Development of the ForteBio™ Octet System for label-free, real-time analysis of molecular interactions
With the resurgence of interest in antibody and protein therapeutics, there is a need to accelerate their characterization and production in a simple, label-free, and information-rich f…
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Scientific Poster
Label-free screening and selection of multiple antibody-antigen pairs for assay development using the Octet System
Label-free screening and selection of multiple antibody-antigen pairs for assay development using the Octet System
One of the challenges in developing immunoassays is to identify suitable antibody pairs that recognize the same target to non-overlapping, non-interfering epitopes. Selection of the mos…
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Scientific Poster
Streamlining assay development with Amine Reactive Biosensors on the Octet System for kinetic analysis of therapeutics
Streamlining assay development with Amine Reactive Biosensors on the Octet System for kinetic analysis of therapeutics
Project timelines and workfl ow are adversely impacted by the amount of assay development required for each protein when using traditional fl ow-cell based biosensors. Several factors c…
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Scientific Poster
Development of the Octet RED System for Label-free, Multi-channel Kinetic Analysis of Biomolecular Interactions
Development of the Octet RED System for Label-free, Multi-channel Kinetic Analysis of Biomolecular Interactions
Traditional methods for analyzing the kinetics of biomolecular binding are often cumbersome and require rigorous sample preparation. In contrast the 96 well plate based instruments in t…
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Scientific Poster
Label-free determination of kinetic constants for small molecule binding to proteins using FortéBio’s Octet Red Multi-Channel Platform
Label-free determination of kinetic constants for small molecule binding to proteins using FortéBio’s Octet Red Multi-Channel Platform
The determination and evaluation of the affinity of small molecule binding to a therapeutic target is a significant component of drug discovery and lead optimization. We report a label-…
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Scientific Poster
Small Molecule Fragment Screening on the Octet Platform
Small Molecule Fragment Screening on the Octet Platform
The Octet RED384 instrument uses biolayer interferometry (BLI) to measure molecular interactions and is well‐suited for label‐free screening of small molecule fragments. It is a 16‐chan…
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Scientific Poster
New label‐free direct detection method for HIS‐tagged protein quantitation
New label‐free direct detection method for HIS‐tagged protein quantitation
The penta‐histidine tag (HIS‐tag) is a common peptide tag fused to recombinant proteins during cloning. Many of the common techniques for detecting these recombinant proteins require bo…
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Scientific Poster
Customized quantitation of recombinant therapeutic proteins using Bio-Layer Interferometry (BLI) and High Precision Streptavidin biosensors (SAX)
Customized quantitation of recombinant therapeutic proteins using Bio-Layer Interferometry (BLI) and High Precision Streptavidin biosensors (SAX)
The accurate determination of recombinant protein titer is critical to the selection of high-producing clones during cell line development and in optimization of bioreactor conditions d…
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Scientific Poster
Fragment Library Screening Using Octet Red
Fragment Library Screening Using Octet Red
We describe, here, the first use of the FórteBio Octet Red and its label-free biolayer interferometry technology for successfully completing a fragment library screening campaign for a…
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Scientific Poster
Detection of STEC Strains O157:H7, O26 and O111 using Biosensor System
Detection of STEC Strains O157:H7, O26 and O111 using Biosensor System
Escherichia coli O157:H7, a strain of enterohemorrhagic, Shiga toxin-producing E. coli (STEC), causes gastroenteritis, resulting in (bloody) diarrhea and sometimes acute kidney failure…
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Scientific Poster
Developing a Chaperonin‐Based Label‐Free Assay Platform to Stabilize Misfolded Proteins
Developing a Chaperonin‐Based Label‐Free Assay Platform to Stabilize Misfolded Proteins
Protein misfolding diseases account for 30-50% of known human diseases. It is possible that chemical ligands that stabilize proteins can be used to treat a significant subset of these f…
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Scientific Poster
Label-free screening strategies using the Octet System from ForteBio
Label-free screening strategies using the Octet System from ForteBio
The flexibility and throughput of ForteBio’s Octet system lends itself readily to a variety of screening applications which will be discussed in detail. Clonal screening and selection…
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Scientific Poster
Quantitation of Residual Protein A and MabSelect SuRe using a “Dip and Read” Assay
Quantitation of Residual Protein A and MabSelect SuRe using a “Dip and Read” Assay
Detection of residual Protein A in product purified using a Protein A, or similar column matrix such as MabSelect SuRe™, is a critical quality control step in antibody therapeutic devel…
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Scientific Poster
Introducing a New BLI Instrument Focused on Throughput and Flexibility: The Octet® HTX System
Introducing a New BLI Instrument Focused on Throughput and Flexibility: The Octet® HTX System
Biolayer Interferometry (BLI) technology has become well established over the past decade for protein quantitation and kinetic characterization of molecular interactions. The non-fluidi…
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Scientific Poster
Influenza vaccine titre determination using Biolayer Interferometry(BLI)
Influenza vaccine titre determination using Biolayer Interferometry(BLI)
Fast, accurate determination of vaccine titre during influenza vaccine manufacture is important in understanding process performance and correctly scaling each process step. Traditional…
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Scientific Poster
Cygnus Anti-CHO HCP Detection Kit for Fast and Easy Detection of Residual CHO Host Cell Proteins on Octet Systems
Cygnus Anti-CHO HCP Detection Kit for Fast and Easy Detection of Residual CHO Host Cell Proteins on Octet Systems
Host cell proteins (HCPs) are contaminants found in biopharmaceuticals expressed in bacterial, yeast or mammalian production cell lines. Among protein expression cell lines, Chinese ham…
Presentations
Determining IgG Concentration by Bio-Layer Interferometry
Determining IgG Concentration by Bio-Layer Interferometry
Bio-Layer Interferometry (BLI) Layer Interferometry (BLI): A layer of molecules attached to the tip of an optical A layer of molecules attached to the tip of an optical fiber creates an…
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Presentations
Improving the Throughput of Titer Determination for PER.C6® Cell Line Generation and Process Development with the Octet Platform
Improving the Throughput of Titer Determination for PER.C6® Cell Line Generation and Process Development with the Octet Platform
Delivery of a high throughput, automatable assay for titer evaluation to support clone selection and culture process development.
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Presentations
Exploring Blocking Assays Using a Parallel Real-time Label-free Biosensor, the Octet
Exploring Blocking Assays Using a Parallel Real-time Label-free Biosensor, the Octet
Knowing how tightly two biomolecules bind one another helps to characterize them…to a point.
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Presentations
Detection of low affinity anti-drug antibodies and improveddrug tolerance in immunogenicity testing by Octet® biolayerinterferometry
Detection of low affinity anti-drug antibodies and improveddrug tolerance in immunogenicity testing by Octet® biolayerinterferometry
ADA immunogenicity assessment is a critical component in demonstrating the safety and efficacy profile of a therapeutic biological drug.
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Presentations
Quantitation of Recombinant Human Factor IX (rFIX) in Bioreactor Harvest Samples using Bio-Layer Interferometry
Quantitation of Recombinant Human Factor IX (rFIX) in Bioreactor Harvest Samples using Bio-Layer Interferometry
The primary use of the rFIX monoclonal sandwich ELISA is to monitor the concentration and batch-to-batch consistency of rFIX in bioreactor harvest samples.
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Presentations
Role of the Sonic Hedgehog Pseudo-Active Site in Signaling
Role of the Sonic Hedgehog Pseudo-Active Site in Signaling
Structural basis of Sonic Hedgehog regulation by Neutralizing Antibody 5E1 and Hedgehog-Interaction Protein.
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Presentations
Binding Rate Screen
Binding Rate Screen
A High-throughput Assay in Soluble Lysate for Prioritizing Protein Expression Constructs
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Presentations
Use of Chaperone Proteins and Octet Bio-Layer Interferometry To Detect Protein Folding /Unfolding Events in a High-Throughput Format
Use of Chaperone Proteins and Octet Bio-Layer Interferometry To Detect Protein Folding /Unfolding Events in a High-Throughput Format
Outline – Chaperonin based interferometry detection platform.
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Presentations
Kinetics on the Octet Systems: What Lies Beneath the Curves
Kinetics on the Octet Systems: What Lies Beneath the Curves
An ‘ideal’ sensorgram recognising non-ideal behaviour. Using more complex models to optimise kinetics assay set-up.
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Presentations
Rapid Assessment of Fab Activity Using Octet BioLayer Interferometry (BLI)
Rapid Assessment of Fab Activity Using Octet BioLayer Interferometry (BLI)
Originally build by Abgenix for multi-product clinical manufacturing, then operated for commercial production by Amgen for 5 years
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Presentations
Using protein thermal denaturation to characterize protein-ligand interactions
Using protein thermal denaturation to characterize protein-ligand interactions
Provide protein chemistry support to National Institutes of Health scientists
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Presentations
Use of Label-free Real-time Biosensors in Characterizing Monoclonal Antibodies
Use of Label-free Real-time Biosensors in Characterizing Monoclonal Antibodies
Develop novel antibody-based medicines across many therapeutic areas from the bench through to the clinic
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Presentations
The Octet and Blitz Systems: Obtaining Kinetics From Crude Media
The Octet and Blitz Systems: Obtaining Kinetics From Crude Media
We have seen many examples of how the Octet systems can measure concentrations directly from crude samples.
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Presentations
Characterisation of a Novel Protein-Lipid Interaction by Biolayer Interferometry
Characterisation of a Novel Protein-Lipid Interaction by Biolayer Interferometry
Characterisation of a Novel Protein-Lipid Interaction by Biolayer Interferometry
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Presentations
Cell-Free Translational Systems for Rapid Species Cross-Reactivity Screening
Cell-Free Translational Systems for Rapid Species Cross-Reactivity Screening
Cell-Free Translational Systems for Rapid Species Cross-Reactivity Screening
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Presentations
BLI Studies of Anthrax Toxin and Development of Diagnostic Antibodies
BLI Studies of Anthrax Toxin and Development of Diagnostic Antibodies
Anthrax is caused by infection with Bacillus anthracis, a spore‐forming, rod‐shaped bacterium Spores are highly resistant to environmental conditions and gain entry via open wound (cuta…
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Presentations
Rapid, Cost Effective Microbial Strain Development Using BLI
Rapid, Cost Effective Microbial Strain Development Using BLI
Located in San Diego, California USA 35 FTEs primarily in SoCal, with satellite offices in St. Louis and Boston 23,000 square feet of space in Sorrento Valley housing capabilities in bi…
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Presentations
High Throughput Label-Free Epitope Binning Made Easy
High Throughput Label-Free Epitope Binning Made Easy
Regeneron is a fully integrated biopharmaceutical company that discovers, invents, develops, manufactures, and commercializes medicines for the treatment of serious medical conditions M…
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Presentations
Human Monoclonal IgG Subclass Determination on the Octet QKe
Human Monoclonal IgG Subclass Determination on the Octet QKe
Monoclonal antibodies (mAbs) are produced from a single cell line. The heavy chain of monoclonal antibodies has a specific isotype subclass. For human IgG there are 4 subclasses: 1,2,3,…
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Presentations
Hit Identification and Lead Confirmation in the Discovery of Drugs Targeting Bromodomain Proteins
Hit Identification and Lead Confirmation in the Discovery of Drugs Targeting Bromodomain Proteins
Family of Chromatin modification “Reader” proteins Recognizing acetylated histone lysine and recruiting “Effectors” (Chromatin modifiers and remodeling enzymes) Critical to maintain pro…
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Presentations
Modular transient protein-protein interaction
Modular transient protein-protein interaction
Cellular reactions and pathways are divided among the individual organelles and cytoplasm, all of which have some size, shape and number. Organelle size should be suited to cellular nee…
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Presentations
MAb Quantitation: Protein A HPLC vs. Protein A Bio-layer Interferometry
MAb Quantitation: Protein A HPLC vs. Protein A Bio-layer Interferometry
MAb Quantitation: Protein A HPLC vs. Protein A Bio-layer Interferometry
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Presentations
Bacterial Surface Receptors and Transporters - Methods for studying their interactions
Bacterial Surface Receptors and Transporters - Methods for studying their interactions
Bacterial Surface Receptors and Transporters - Methods for studying their interactions
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Presentations
Structural Studies of an HIV Env Trimer Enabled and Enriched by Biophysical Analyses
Structural Studies of an HIV Env Trimer Enabled and Enriched by Biophysical Analyses
Structural Studies of an HIV Env Trimer Enabled and Enriched by Biophysical Analyses
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Presentations
Application of Label Free Technology for Biologics Discovery
Application of Label Free Technology for Biologics Discovery
Global biologics research and development arm of AstraZeneca Approximately 2,500 full-time employees in the US and UK Robust pipeline of more than 120 biologics in research and developm…
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Presentations
Octet RED: An Antibody Discovery Case Study
Octet RED: An Antibody Discovery Case Study
Octet RED: An Antibody Discovery Case Study
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Presentations
In Vitro Assay to Predict Human IgG Half-Life
In Vitro Assay to Predict Human IgG Half-Life
For therapeutic mAbs, longer half-life enhances: Safety Efficacy Cost Convenience For diagnostic mAbs, shorter half-life is usually desired. Lack of cost effective, high-throughput ass…
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Presentations
Seeing is Believing: Analyzing Protein Complexes Assembled on and Released from Biolayer interferometry Biosensor Surfaces using Mass Spectroscopy and Electron Microscopy
Seeing is Believing: Analyzing Protein Complexes Assembled on and Released from Biolayer interferometry Biosensor Surfaces using Mass Spectroscopy and Electron Microscopy
Analyzing protein complexes assembled on and isolated from BLI biosensor using EM and MS
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Presentations
BLI Technology to Assess Quality and Compatibility of Commercial Antibodies from Multiple Vendors
BLI Technology to Assess Quality and Compatibility of Commercial Antibodies from Multiple Vendors
Founded in 2013 and resident of the Marcus Institute of Nanotechnology at Georgia Institutes of Technology in Atlanta Annular’s research team has over thirty years combined experience i…
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Presentations
Assessment of the FortéBIO Octet System as a Means to Measure AntiDrug Antibodies
Assessment of the FortéBIO Octet System as a Means to Measure AntiDrug Antibodies
CNTO X is a therapeutic human IgG1 that neutralizes an inflammatory cytokine
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Presentations
Octet Kinetic Analysis as an Integral Step in the Therapeutic Antibody Screening and Selection Process
Octet Kinetic Analysis as an Integral Step in the Therapeutic Antibody Screening and Selection Process
ForteBio Octet Kinetic Analysis as an Integral Step in the Therapeutic Antibody Screening and Selection Process
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Presentations
Bridging Artifacts in Octet Measurements: Detecting them, avoiding them, & compensating for them
Bridging Artifacts in Octet Measurements: Detecting them, avoiding them, & compensating for them
Bridging Artifacts in Octet Measurements: Detecting them, avoiding them, & compensating for them
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Presentations
Octet as a reliable novel biosensor in characterizing protein/protein interactions
Octet as a reliable novel biosensor in characterizing protein/protein interactions
Introducing the Octet as a reliable novel biosensor in characterizing protein/protein interactions
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Presentations
Screening and characterization of fully human antibodies from Velocimmune mice using realtime label-free interaction analysis
Screening and characterization of fully human antibodies from Velocimmune mice using realtime label-free interaction analysis
Regeneron is a medium-sized fully integrated biopharmaceutical company that discovers, develops, and commercializes medicines for the treatment of serious medical conditions.
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Presentations
Host Cell Protein Analysis on the ForteBio Octet Platform
Host Cell Protein Analysis on the ForteBio Octet Platform
Heterogeneous population of proteins that represents the four quadrants of a two dimensional gel
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Presentations
The use of Bio-Layer Interferometry (BLI) for Quantitation of a Humanized Antibody Therapeutic
The use of Bio-Layer Interferometry (BLI) for Quantitation of a Humanized Antibody Therapeutic
Coated with proprietary biocompatible matrix that is uniform, nondenaturing, and has minimal non-specific binding.
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Presentations
Epitope Binning for Antibody Characterisation on the Pall FortéBio Octet RED384 System
Epitope Binning for Antibody Characterisation on the Pall FortéBio Octet RED384 System
Introduction to UCB’s core antibody discovery platform Utilising the Octet in our workflow Cross-blocking to characterise antibodies Sequential binding experiments
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Presentations
Analysis of novel binders to soluble proteins in serum using BLI technology
Analysis of novel binders to soluble proteins in serum using BLI technology
Maturated via randomization of 13 surface exposed amino acids followed by phage display to gain high affinity for the chosen target protein (complement factor C5).
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Presentations
High-Throughput Characterization of Monoclonal Antibodies Using the Octet HTX System
High-Throughput Characterization of Monoclonal Antibodies Using the Octet HTX System
Antibody Core Facility at Novo Nordisk, providing poly- and monoclonal antibodies for multiple purposes:
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Presentations
Antigen and antibody development for IVD immunoassay using Octet Biolayer Interferometry
Antigen and antibody development for IVD immunoassay using Octet Biolayer Interferometry
Viral, bacterial and parasitological culture and purification Synthetic peptides and recombinant antigen production and purification Rabbit, goat, guinea pig and lamb polyclonal antibod…
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Presentations
Selection of monoclonal antibodies using the Octet platform for diagnostic application : strengths and limitations
Selection of monoclonal antibodies using the Octet platform for diagnostic application : strengths and limitations
Use of Octet platform for diagnostic application at Beckman Coulter: Selection of monoclonal antibodies with high sensitivity, low dissociation and no cross-reactivity First screening o…
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Presentations
Peptides of Presenilin-1 offer a novel and specific therapeutic approach to reduce ß-amyloid in Alzheimer’s Disease
Peptides of Presenilin-1 offer a novel and specific therapeutic approach to reduce ß-amyloid in Alzheimer’s Disease
ß-amyloid (Aß) Considered to be the Major Toxic Species in Alzheimer’s Disease
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Presentations
Integration of Octet RED384 system into the Emory Chemical Biology Discovery Center for Small Molecule Discovery
Integration of Octet RED384 system into the Emory Chemical Biology Discovery Center for Small Molecule Discovery
Emory Chemical Biology Discovery Center (ECBDC) Integration of Octet RED384 into ECBDC for small molecule discovery Applications of Octet RED384 Characterization of small molecule 14-3-…
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Presentations
Streamlining methods for screening antibody performance
Streamlining methods for screening antibody performance
The Edgewood Chemical Biological Center's (ECBC) science and technology expertise has protected the United States from the threat of chemical weapons since 1917. Since that time, ECBC h…
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Presentations
Regenerable Biosensor-based Hit-validation for DNA-encoded Chemical Library Selection
Regenerable Biosensor-based Hit-validation for DNA-encoded Chemical Library Selection
Explore this document which describes Regenerable Biosensor-based Hit-validation for DNA-encoded Chemical Library Selection.
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Presentations
Using the Octet RED with Virus-like Particles to Study Binding to Intact Membrane Proteins
Using the Octet RED with Virus-like Particles to Study Binding to Intact Membrane Proteins
The document explores the use of Octet RED with Virus-like Particles which help in studying Binding to Intact Membrane Proteins.
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Presentations
An Octet RED Based Method for Kinetic Analysis of Novel Binders to Soluble Serum Proteins Using Serial Dilutions of Serum Without Purification of the Target
An Octet RED Based Method for Kinetic Analysis of Novel Binders to Soluble Serum Proteins Using Serial Dilutions of Serum Without Purification of the Target
Our world‐class capabilities in protein biochemistry and biologics manufacturing will allow us to develop next generation biological products.
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Presentations
Liposome Binding Assays
Liposome Binding Assays
Department of Biotechnology - Identifying and developing novel compounds and processes for biopharmaceuticals Head of Department: Ao.Univ.Prof. Dr. Rücker Florian
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Presentations
Drugging the orphan Nuclear Receptor TLX with the Octet-Red System
Drugging the orphan Nuclear Receptor TLX with the Octet-Red System
TLX is overexpressed in brain tumors stem cells, various human glioma cell lines and its overexpression in human patients correlates with a poor prognosis
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Presentations
Using BLI to measure binding kinetics of non-natural products
Using BLI to measure binding kinetics of non-natural products
My participation in the Pall ForteBio User Meeting is not intended as indicating official support or endorsement by the U.S. government or the U.S Department of Defense of Pall ForteBio…
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Presentations
Application of Octet for Rapid Screening of Assay Reagents for Pharmacokinetic Ligand Binding Assays for Antibody Drug Conjugates
Application of Octet for Rapid Screening of Assay Reagents for Pharmacokinetic Ligand Binding Assays for Antibody Drug Conjugates
Develop and Validate Ligand Binding Assays (LBAs) for Large Molecule Therapeutics from Discovery through Clinical.
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Presentations
Bio-Layer Interferometry: Reporting Kinetic Effects of Small Ligands on Protein-Protein Interactions
Bio-Layer Interferometry: Reporting Kinetic Effects of Small Ligands on Protein-Protein Interactions
Bacterial system as a model for studying all F-type ATP synthases simplest subunit composition all subunits have homologs in mitochondrial complex best system for genetic, mutagenic stu…
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Presentations
The Role The N-Terminal Domain Plays in Spidroin Assembly
The Role The N-Terminal Domain Plays in Spidroin Assembly
This document describes the role the N-Terminal domain plays in spidroin assembly. Explore more about NTD Dimer.
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Presentations
A Method to Determine the Reversibility of Inhibition for Glutamate Carboxypeptidase II Inhibitors using BioLayer Interferometry
A Method to Determine the Reversibility of Inhibition for Glutamate Carboxypeptidase II Inhibitors using BioLayer Interferometry
Most commonly diagnosed cancer among US men and accounts for the second leading cause of death
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Presentations
A High Throughput Approach To Quantify Fab Yields In a Heterogeneous Mixture Of Monomer And Dimer
A High Throughput Approach To Quantify Fab Yields In a Heterogeneous Mixture Of Monomer And Dimer
Next-Generation Antibody Platform AbSci’s production technology, SoluProTM, embodies the best of E. coli and mammalian expression systems
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Presentations
MAP kinase regulation of endothelial nitric oxide synthase
MAP kinase regulation of endothelial nitric oxide synthase
NOSs are a family of enzymes that synthesize NO from L-arginine Found in bacteria, invertebrates, fish, birds, mammals 3 mammalian types, endothelial (eNOS), neuronal (nNOS) and inducib…
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Presentations
Automated Optimization of IgGProduction in CHO Cell Line Development
Automated Optimization of IgGProduction in CHO Cell Line Development
Biomek Automated Workstations are not intended or validated for use in the diagnosis of disease or other conditions. Beckman Coulter Life Sciences genomic reagent kits are for research…
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Presentations
Ligand Affinity Measurements for the Community Structure Activity Resource Center(CSAR)
Ligand Affinity Measurements for the Community Structure Activity Resource Center(CSAR)
The goal is to increase the amount of high quality data publicly available for development, validation, and benchmarking of ligand docking and screening software.
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Presentations
Octet, Your Friend in Vaccine Development: Assessing Antigenicity and Immunogenicity of HIV-1 Envelope Proteins by Bio-Layer Interferometry
Octet, Your Friend in Vaccine Development: Assessing Antigenicity and Immunogenicity of HIV-1 Envelope Proteins by Bio-Layer Interferometry
Located in Rockville, MD CMO/CRO that provide diverse services for biologics development, virus/protein GMP manufacturing, and clinical testing Approximately 200 employees in U.S. (Mary…
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Presentations
Implementation of the Octet RED384 in a High Throughput mAb Generation Platform
Implementation of the Octet RED384 in a High Throughput mAb Generation Platform
Antibody Technology: Antibody Core Facility at Novo Nordisk, providing poly- and monoclonal antibodies for multiple purposes; Therapeutic monoclonal antibodies; Immunoassays
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Presentations
Dip and Read Immunogenicity Assay
Dip and Read Immunogenicity Assay
Traditional Methods for ADA Testing Binding Assay – ELISA (2D, 3D, ECL) Radio-Immunoprecipitation Assay SPR Immunoblotting
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Presentations
Generating Conformation- and Mutation-Specific Antibodies to Disrupt Oncogenic RAS Signaling
Generating Conformation- and Mutation-Specific Antibodies to Disrupt Oncogenic RAS Signaling
Learn about Specific Generating Conformation and Mutation-Antibodies to Disrupt Oncogenic RAS Signaling. Know more about Conformational Dynamics of RAS.
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Presentations
LigandBinding Assays on the Octet Platform for Bioprocess Contaminants: Residual Protein A and HCP
LigandBinding Assays on the Octet Platform for Bioprocess Contaminants: Residual Protein A and HCP
The flexible Octet platform provides various assay formats to modulate the dynamic rangeand the sensitivity, enabling many high sensitive contamination assays on a single platform simpl…
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Presentations
Fc-receptor Binding Assays
Fc-receptor Binding Assays
Fc receptors belong to the immunoglobulin superfamily and bind to the Fc-part of antibodies.
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Presentations
Discovery and Fine-tuning of Aptamer Based Diagnostic Agents
Discovery and Fine-tuning of Aptamer Based Diagnostic Agents
This document helps you to discover and fine-tune aptamer based diagnostic agents which is the main octet functions at AM Biotech.
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Presentations
De-orphanizing a Nuclear Receptor with the Octet-Red System
De-orphanizing a Nuclear Receptor with the Octet-Red System
Explore more about Nuclear Receptors Superfamily and learn how De-orphanizing a Nuclear Receptor with the Octet-Red system happens.
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Presentations
Rapid Detection and Screening of His-tagged Proteins using Simple, Label-Free “Dip and Read” Assay
Rapid Detection and Screening of His-tagged Proteins using Simple, Label-Free “Dip and Read” Assay
Bio layer Interferometry technology minimizes extraneous sample material interference in crude mixtures such as serum, cell lysate and hybridoma supernatants and reduces sample preparat…
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Presentations
Computational Design of Protein‐ Protein Interactions
Computational Design of Protein‐ Protein Interactions
Computational protein design methods Using a purpose‐built protein scaffold: Design of a cancer‐specific toxin Intracellular delivery of a designer toxin: a new generation of antibody‐d…
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Presentations
Use of BLI in nucleic acid aptamer selection, maturation and characterisation
Use of BLI in nucleic acid aptamer selection, maturation and characterisation
This document provides information about Aptamer and use of BLI in nucleic acid aptamer selection, maturation and characterization.
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Presentations
Biosensor-based Fragment Screening with Protein-protein Interaction (PPI) Targets
Biosensor-based Fragment Screening with Protein-protein Interaction (PPI) Targets
Protein-protein interactions are key regulators of disease biology – “Biological gold mine”…..Asher Mullard Nature Rev Drug Disc 2012 PPI targets are challenging because they lack well-…
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Presentations
Bio-Layer Interferometry Technology Applied to Fragment Screening
Bio-Layer Interferometry Technology Applied to Fragment Screening
Most of human proteins do not work on their own but in complex with other protein(s), PPIs are central to all the biological processes and often dysregulated in disease – the possibilit…
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Presentations
Assessment of Monoclonal Antibody/FcReceptor Interactions Using Octet
Assessment of Monoclonal Antibody/FcReceptor Interactions Using Octet
Assessment of biopharmaceutical therapeutic molecules, or their associated derivative biosimilar molecules, requires an orthogonal analytical approach.
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Presentations
About Antibody Solutions
About Antibody Solutions
CRO in SF Bay Area Therapeutic Ab Discovery Critical Reagent Abs (e.g. PK, PD, ADA, BioMarker) Omni-Rat & Omni-Mouse Human Transgenic Ab Platform
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Presentations
Characterization of the Antibody Quantitation Assay with the Octet Red
Characterization of the Antibody Quantitation Assay with the Octet Red
Bio-Layer Interferometry (BLI)
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Presentations
Comparison of Concentration and Kinetic Analysis of Protein Therapeutics Using Free Labeling Assay Platforms: Biacore, Forte’Bio and ProteOn XPR 36
Comparison of Concentration and Kinetic Analysis of Protein Therapeutics Using Free Labeling Assay Platforms: Biacore, Forte’Bio and ProteOn XPR 36
Employ new technologies, such as Forte Bio’s Octet optical biosensor, which utilizes proprietary Bio-LayerInterferometry, to detect and quantify molecular interaction that addresses sam…
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Presentations
Characterization of Therapeutic Antibodies Derived from a Novel Yeast Expression Platform Using Bio-Layer Interferometry (BLI)
Characterization of Therapeutic Antibodies Derived from a Novel Yeast Expression Platform Using Bio-Layer Interferometry (BLI)
Antibodies are successful therapeutics
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Presentations
Establishing a Platform Ligand-Binding Method on ForteBio Octet
Establishing a Platform Ligand-Binding Method on ForteBio Octet
About KBI: A Contract Development and Manufacturing Company KBI helps partners accelerate and optimize drug development and manufacturing programs with an extensive suite of expert deve…
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Presentations
Kinetic Characterization of Protein-Protein Interactions in Purified and Bacterial Lysates
Kinetic Characterization of Protein-Protein Interactions in Purified and Bacterial Lysates
Octet RED instrument to provide kinetic characterization of clones at the screening/discovery level. Both purified protein and bacterial extract samples are analyzed. Kinetic results ar…
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Presentations
Applications of Octet RED96 In Peptide Discovery and CovX-Body Characterization
Applications of Octet RED96 In Peptide Discovery and CovX-Body Characterization
CovX-Bodies combine advantages of therapeutic peptides and antibodies. CovX peptide discovery process enables rapid identification and optimization of novel peptide therapeutics. Octet…
Product Insert
Biosensor Mount Cleaning Tray for Octet HTX, RED384, and QK384 Systems
Biosensor Mount Cleaning Tray for Octet HTX, RED384, and QK384 Systems
Biosensor Mount Cleaning Trays (“Cleaning Trays”) from Pall ForteBio are intended for periodic, regular, automated cleaning of biosensor mounts on Octet HTX, RED384, and QK384 instrumen…
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Product Insert
Protein A Biosensors
Protein A Biosensors
Protein A is a cell wall protein derived from Staphylococcus aureus that exhibits unique binding properties for immunoglobulins in a variety of mammalian species. In particular, Protein…
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Product Insert
Amine Coupling Reagent Kit
Amine Coupling Reagent Kit
This kit is intended to provide the Octet System user with a convenient source for all the reagents needed to immobilize a protein of interest onto the Amine Reactive Biosensors. The ki…
Technical Guide
Octet System
Octet System
The FortéBio Octet System includes the Octet instrument and Octet software, as well as the Origin® software, a graphing and analysis application for kinetic data analysis. The Octet Sys…
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Technical Guide
Octet RED Software Version 5.2
Octet RED Software Version 5.2
Octet software version 5.2 offers a range of data analysis features to enable a more streamlined and more flexible data analysis workflow. These features provide more options to the end…
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Technical Guide
Sidekick™ Offline Biosensor Immobilization Station
Sidekick™ Offline Biosensor Immobilization Station
The Sidekick Station is an accessory to Pall ForteBio's Octet family of real-time, label-free biomolecular interaction analysis platforms. It is a specialized instrument that enables un…
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Technical Guide
Anti-Human Fab-CH1 Biosensor Kinetics Assays
Anti-Human Fab-CH1 Biosensor Kinetics Assays
The Anti-Human Fab-CH1 Biosensor consists of a high-specificity anti-Human Fab-CH1 antibody fragment pre-immobilized on a ForteBio biosensor. In conjunction with the Octet® or BLItz™ sy…
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Technical Guide
Anti-Human Fab-CH1 (FAB) Biosensor Quantitation Assays
Anti-Human Fab-CH1 (FAB) Biosensor Quantitation Assays
The Anti-Human Fab-CH1 Biosensor consists of a high affinity antihuman CH1 affinity ligand pre-immobilized on a ForteBio biosensor. In conjunction with the Octet® or BLItz™ systems, the…
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Technical Guide
Octet® System Data Analysis
Octet® System Data Analysis
Octet systems enable real-time quantitation or kinetic characterization of biomolecular interactions
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Technical Guide
Octet® System Data Acquisition
Octet® System Data Acquisition
Octet systems enable real-time quantitation or kinetic characterization of biomolecular interactions.
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Technical Guide
Octet® Data Analysis High Throughput (HT) User Guide
Octet® Data Analysis High Throughput (HT) User Guide
Octet Data Analysis High Throughput (HT) software lets you analyze multiple data files at once. You can look at multiple experiments, biosensor trays or plates from the same or differen…
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Technical Guide
Biotinylate antibody from stock which contains carrier protein
Biotinylate antibody from stock which contains carrier protein
Biotinylate antibody from stock which contains carrier protein (Recommended procedure for use with FortéBio streptavidin biosensors)
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Technical Guide
Rapid Analysis of Fab Fragments and IgG with Anti-Human Fab-CH1 2nd Generation (FAB2G) Biosensors
Rapid Analysis of Fab Fragments and IgG with Anti-Human Fab-CH1 2nd Generation (FAB2G) Biosensors
Anti-Human Fab-CH1 2nd Generation (FAB2G) biosensors from Pall ForteBio come pre-immobilized with a high affinity ligand that is specific for the CH1 region of Human IgG. In conjunction…
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Technical Guide
Batch Immobilization of a Biotinylated Ligand onto Streptavidin Biosensors
Batch Immobilization of a Biotinylated Ligand onto Streptavidin Biosensors
ForteBio Streptavidin biosensors enable the immobilization of a biotinylated ligand onto the biosensor surface. The immobilized molecule can then be used in subsequent kinetic or custom…
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Technical Guide
Biotinylating Antibody in Stocks Containing Carrier Protein
Biotinylating Antibody in Stocks Containing Carrier Protein
The interaction between streptavidin and biotin is widely used as a system for the rapid, stable, and irreversible non-covalent binding of proteins to ligands. The Streptavidin biosenso…
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Technical Guide
Regeneration Strategies for Streptavidin Biosensors on the Octet Platform
Regeneration Strategies for Streptavidin Biosensors on the Octet Platform
The ForteBio Octet platform offers powerful tools for the real-time, label-free analysis of protein-protein interactions. Together, the Octet systems and Streptavidin (SA) biosensors pr…
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Technical Guide
Immunogenicity Assays on the Octet Platform
Immunogenicity Assays on the Octet Platform
Immunogenicity testing, the measurement of an immune response to a therapeutic drug, is an integral part of drug development. The immune system may respond to drug administration in pat…
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Technical Guide
Human and Mouse IgG Subtype Identification Using the Octet™ Platform
Human and Mouse IgG Subtype Identification Using the Octet™ Platform
This technical note describes a rapid assay method for quantitation and subtype identification of human and mouse IgG. The antibodies can be quantified in buffer, serum-free media or ly…
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Technical Guide
Protein G Biosensors
Protein G Biosensors
Protein G biosensors provide a rapid and direct method for quantification of many types of mammalian IgG from buffer, media or other complex matrices. Protein G, which is pre-immobilize…
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Technical Guide
Dip and Read™ Protein L Biosensors
Dip and Read™ Protein L Biosensors
Protein L biosensors provide a rapid and direct method for quantifying a broad set of kappa light chain containing immunoglobulins, including whole molecules, FAb fragments and single c…
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Technical Guide
Host Cell Protein Detection
Host Cell Protein Detection
Host cell proteins (HCPs) are contaminants found in biopharmaceuticals that originate from the production cell lines of bacterial, yeast or mammalian cell culture. HCPs can reduce drug…
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Technical Guide
Dip and Read™ Anti-Mouse IgG Fc Capture Biosensors
Dip and Read™ Anti-Mouse IgG Fc Capture Biosensors
Anti-Mouse IgG Fc Capture (AMC) biosensors enable kinetic characterization of macromolecular interactions between mouse Fc-containing proteins and target analytes (Figure 1). Immobiliza…
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Technical Guide
Dip and Read™ Amine Reactive Second-Generation (AR2G) Biosensors
Dip and Read™ Amine Reactive Second-Generation (AR2G) Biosensors
Amine Reactive 2nd Generation (AR2G) biosensors enable kinetic characterization of macromolecular interactions between purified proteins and target analytes (Figure 1). Immobilization o…
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Technical Guide
Dip and Read™ Amine Reactive Second-Generation (AR2G) Reagent Kit
Dip and Read™ Amine Reactive Second-Generation (AR2G) Reagent Kit
The Amine Reactive 2nd Generation (AR2G) Reagent Kit is intended for use with the AR2G biosensor to enable covalent immobilization of a protein, peptide or other primary amine containin…
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Technical Guide
Biotinylation of Protein for Immobilization onto Streptavidin Biosensors
Biotinylation of Protein for Immobilization onto Streptavidin Biosensors
The interaction between streptavidin and biotin is widely used as a system for the rapid, stable and irreversible non-covalent binding of biological molecules. The Octet® platform’s Str…
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Technical Guide
GST Biosensor Kinetics Assays
GST Biosensor Kinetics Assays
Glutathione-S-transferase (GST) is commonly fused to recombinant proteins to facilitate detection and purification, and to increase solubility. The anti-GST biosensor consists of a high…
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Technical Guide
Anti-GST Biosensor Quantitation Assays
Anti-GST Biosensor Quantitation Assays
Glutathione-S-transferase (GST) is commonly fused to recombinant proteins as a means of facilitating detection, purification and increasing solubility. The anti-GST biosensor consists o…
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Technical Guide
Ni-NTA Biosensor Kinetic Assays
Ni-NTA Biosensor Kinetic Assays
A polyhistidine tag (also known as hexa histidine-tag, 6xHIS-tag, or by the trademarked name HIS-tag) is commonly fused to recombinant proteins to facilitate their detection and purific…
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Technical Guide
Ni-NTA Biosensor Quantitation Assays
Ni-NTA Biosensor Quantitation Assays
A polyhistidine tag (also known as hexa histidine-tag, 6xHIS-tag, or by the trademarked name HIS-tag) is commonly fused to recombinant proteins to facilitate their detection and purific…
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Technical Guide
Anti-HIS (HIS2) Biosensor Quantitation Assays
Anti-HIS (HIS2) Biosensor Quantitation Assays
The polyhistidine-tag (HIS-tag) is a common peptide tag fused to recombinant proteins during cloning. Many tools have been developed that enable this tag to be used for detection and pu…
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Technical Guide
Anti-Penta-HIS (HIS1K) Biosensors for Label-free Analysis of His-tagged Proteins
Anti-Penta-HIS (HIS1K) Biosensors for Label-free Analysis of His-tagged Proteins
The polyhistidine-tag (His-tag) is a common peptide tag added to recombinant proteins during cloning. Many tools have been developed that enable this tag to be used for detection and pu…
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Technical Guide
Analysis of FLAG Proteins on the Octet® Platform Using SAX Biosensors
Analysis of FLAG Proteins on the Octet® Platform Using SAX Biosensors
The DYKDDDDK polypeptide tag, commonly known as the FLAG® tag, is fused to recombinant proteins to facilitate their detection and purification. Pall ForteBio’s High Precision Streptavid…
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Technical Guide
Octet kinetics assay: method development guideline
Octet kinetics assay: method development guideline
The purpose of this document is to describe the procedure for developing a ligand binding kinetics assay on Octet® QKe, RED96, RED96e, RED384 and HTX instruments. The procedures describ…
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Technical Guide
Ready BLI Detection Kit – Residual Protein A
Ready BLI Detection Kit – Residual Protein A
The Ready BLI Detection kit for Residual Protein A is intended for the detection and quantitation of recombinant Protein A or other Protein A constructs such as MabSelect SuRe™. It has…
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Technical Guide
Enabling Depth Filter Selection with Rapid Detection of Monoclonal Antibodies in Culture Using the BLItz System
Enabling Depth Filter Selection with Rapid Detection of Monoclonal Antibodies in Culture Using the BLItz System
Harvesting and recovering monoclonal antibodies (mAb) from mammalian cell culture bioreactor samples is just the first step of a closely coordinated chain of purification and processing…
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Technical Guide
Guidelines for OneStep Protocol Design
Guidelines for OneStep Protocol Design
The Pioneer and Pioneer FE Next Generation SPR systems by ForteBio employ an innovative gradient injection technology based on Taylor dispersion called OneStep®. A OneStep injection pro…
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Technical Guide
High Precision Streptavidin Biosensor (SAX) Quantitation and Kinetic Assays
High Precision Streptavidin Biosensor (SAX) Quantitation and Kinetic Assays
Streptavidin-coated surfaces are widely used as a simple and straightforward method of molecular immobilization. Utilized with Bio-Layer Interferometry (BLI), Streptavidin biosensors en…
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Technical Guide
Small Molecule Binding Kinetics
Small Molecule Binding Kinetics
Small molecule kinetics can be measured on the Octet RED, Octet RED96 and Octet RED384 instruments. In a typical experiment, a biotinylated protein target is immobilized onto a high-cap…
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Technical Guide
CHO Host Cell Protein Detection
CHO Host Cell Protein Detection
Host cell proteins (HCPs) are contaminants found in biopharmaceuticals expressed in bacterial, yeast or mammalian production cell lines. Among protein expression cell lines, Chinese ham…
White Paper
Automating Octet Assays : ligand screening at Avitide
Automating Octet Assays : ligand screening at Avitide
Drug development and production is challenging. Avitide, based in Lebanon, New Hampshire, provides on-demand development and supply of high-performance affinity purification resins for…
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White Paper
Streamlining affinity analysis for accelerated lead screening, characterization, optimization and final selection
Streamlining affinity analysis for accelerated lead screening, characterization, optimization and final selection
Antibody and other protein therapeutics are a major focus in drug discovery pipelines today. The overall process for developing protein therapeutics encompasses target selection and val…
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White Paper
Writing an Equipment Grant Proposal for Biomolecular Interactions Research: Bio-Layer Interferometry (BLI) and Octet Systems
Writing an Equipment Grant Proposal for Biomolecular Interactions Research: Bio-Layer Interferometry (BLI) and Octet Systems
In this white paper, guidelines for preparing an instrumentation grant application will are provided for principal investigators interested in acquiring an Octet® system, the label-free…
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White Paper
Octet platform: a complete solution for lot release and in-process testing of biologics in GxP laboratories
Octet platform: a complete solution for lot release and in-process testing of biologics in GxP laboratories
Bio-Layer Interferometry (BLI) is an optical analytical technique that analyzes the interference pattern of white light reflected from two surfaces: a layer of immobilized protein on th…
Publications
Current Biosensor Technologies in Drug Discovery
Current Biosensor Technologies in Drug Discovery
Over the past two decades the benefits of biosensor analysis have begun to make an impact in the market, and systems are beginning to be used as mainstream research tools in many labora…
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Publications
Immunogenicity of a Prefusion HIV-1 Envelope Trimer in Complex with a Quaternary-Structure-Specific Antibody
Immunogenicity of a Prefusion HIV-1 Envelope Trimer in Complex with a Quaternary-Structure-Specific Antibody
The HIV-1 envelope trimer (Env) is the main target of virus-directed neutralizing antibodies. One of the commonly used glycoprotein-mimics of HIV-1 Env is the SOSIP.664-stabilized solub…
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Publications
Restoring Anticancer Immune Response by Targeting Tumor-Derived Exosomes With a HSP70 Peptide Aptamer
Restoring Anticancer Immune Response by Targeting Tumor-Derived Exosomes With a HSP70 Peptide Aptamer
According to recent studies, myeloid-derived suppressor cells (MDSCs) suppress tumor immunity of cancer patients. Exosomes are cell-derived nanovesicles that are discharged into the ext…
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Publications
Design, Synthesis, and Biological Evaluation of Novel FAK Scaffold Inhibitors Targeting the FAK-VEGFR3 Protein-protein Interaction
Design, Synthesis, and Biological Evaluation of Novel FAK Scaffold Inhibitors Targeting the FAK-VEGFR3 Protein-protein Interaction
The authors designed, synthesized and screened for potent and selective tyrosine kinase inhibitors of focal adhesion kinase (FAK) and vascular endothelial growth factor receptor 3 (VEGF…
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Publications
Detection of Low-Affinity Anti-Drug Antibodies and Improved Drug Tolerance in Immunogenicity Testing by Octet Biolayer Interferometry
Detection of Low-Affinity Anti-Drug Antibodies and Improved Drug Tolerance in Immunogenicity Testing by Octet Biolayer Interferometry
The authors compare performance of the Octet system to ELISA and MSD techniques for detection of anti-drug antibodies (ADA) against an investigational therapeutic human IgG1 mAb called…
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Publications
Towards a Universal Disulphide Stabilised Single Chain Fv Format: Importance of Interchain Disulphide Bond Location and vL-vH Orientation
Towards a Universal Disulphide Stabilised Single Chain Fv Format: Importance of Interchain Disulphide Bond Location and vL-vH Orientation
Five disulphide bond locations were introduced into one scFv in order to compare their relative effects on expression, thermal stability, % monomer formation and retention of antigen bi…
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Publications
Off-rate Screening for Selection of High-affinity Anti-drug Antibodies
Off-rate Screening for Selection of High-affinity Anti-drug Antibodies
To aid in the development of high-affinity anti-drug antibodies, the authors developed a method for off-rate screening of crude E. coli lysate containing monovalent Fab fragments obtain…
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Publications
A Novel Rabbit Monoclonal Antibody Platform To Dissect the Diverse Repertoire of Antibody Epitopes for HIV-1 Env Immunogen Design
A Novel Rabbit Monoclonal Antibody Platform To Dissect the Diverse Repertoire of Antibody Epitopes for HIV-1 Env Immunogen Design
In this study, 12 HIV-1 Env-specific rabbit hybridoma cell lines were produced that recognized a wide range of Env epitopes. Heavy-chain and light-chain genes were cloned and their gene…
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Publications
Identify the Key Amino Acid of BAFF Binding with TACI
Identify the Key Amino Acid of BAFF Binding with TACI
In this study, the key amino acids involved in the binding of B-cell activating factor (BAFF) to its receptor TACI were identified. TACI binding kinetics for various BAFF mutants were s…
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Publications
Ferredoxin Competes with Bacterial Frataxin in Binding to the Desulfurase IscS
Ferredoxin Competes with Bacterial Frataxin in Binding to the Desulfurase IscS
The authors have characterized the interactions of the bacterial desulferase IscS and the specialized ferredoxin Fdx using a combination of biophysical tools and mutagenesis. The Octet…
Videos and Demos
Cell-based Label-free Monitoring of Signal Transduction
Fast and Flexible Analysis of Fc-Gamma Receptor Binding Interactions
Flexible Monitoring of Small Molecules with Aptamer Affinity Reagents Using Bio-Layer Interferometry
Kinetics on the Octet Systems What Lies Beneath the Curves
Best Practices for Large Molecule Kinetic Assays on the Octet System
Protein Concentration Measurements in Crude Samples in a Dip and Read Format
The Octet Platform A Versatile Instrument in Downstream Processing
Cross-competition or Epitope Binning Assays on the Octet HTX System
Automated workflow solutions for the Octet HTX and RED384 systems
Related Products & Services of High Throughput Octet Systems: Octet HTX and Octet RED384
Featured Applications
ELISA Conversion
Despite its widespread adoption in industry, variability in results, susceptibility to human error…
Epitope Binning
Epitope binning studies rely on the sequential binding of two antibodies to an antigen, and are…
Protein Contaminant Testing
Protein contaminants are a major hurdle to overcome during the production of biopharmaceuticals.…
Customer Breakthrough
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Mabtech AB uses the Octet RED384 system to help optimize the selection of new antibody producing cells
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