SLAS 2023 快照:创新、经验教训以及您可能错过的内容

We caught up with Laura Dranschak, Director of Commercial Operations & Custom Solutions, to catch up on all things SLAS 2023. 了解展会现场的热门话题、自动化行业对未来实验室的影响,并下载我们最新的 3D 复杂检测自动化研究海报。

    • Our own innovative solutions showcased at SLAS were driven by customers as well. For instance, the QPix XE, a smaller version of our industry leading QPix automated colony picker, is a direct result of customer requests for a bench top colony picker that is affordable for academics and startups. Artificial intelligence (AI) is rapidly evolving—faster, better, and increasingly flexible science backed by data analytics and customer 360 insight, enabling a holistic approach to customer workflows.

Take a look at the automation solutions shared at the show

Automating patient-derived colorectal cancer organoids for high-throughput screening

Presented by Angeline Lim, PhD

For this poster, we developed an end-to-end, automated workflow starting with assay-ready colorectal cancer organoids that tracked the effects of various compounds on colorectal organoid size, morphology, texture, and additional morphological and phenotypic readouts. Overall, our results show the superior potential of PDOs vs. other tissues in both precision medicine and high-throughput drug discovery applications when using automation with high-content imaging.

下载海报

https://share.vidyard.com/watch/bxuJntDLDZT3HZ5iDiCqmM

3D 生物打印测定自动化可实现高内涵成像和化合物效应评估

https://share.vidyard.com/watch/vvUhWov5B43qRNrXyUFyCf

Presented by Prathyushakrishna Macha, PhD

The automation of a 3D cell model results in a significant reduction in the time and effort involved, as well as an increase in assay precision and throughput. Here we describe methods for an automated generation of organoids and 3D models using automated 3D bioprinting. An increase in throughput and ease of operation was achieved through automation. Also, imaging and data analysis methods provided valuable information about complex compound effects in 3D printed and cell-tissue-engineered models

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Leveraging Automated Workflows to Enable Complex Organoid Assays

Presented by Oksana Sirenko, PhD

In this poster we demonstrate the tools for increasing throughput and automation in organoid assays and provide strategies aimed at enabling reliable, scalable, and reproducible cell culturing, monitoring, imaging, and analysis of complex biological models.

下载海报

https://share.vidyard.com/watch/JaycrPGzWvXXoUmYqo4Kqo

Novel analysis of neural outgrowth in 3D human brain micro-tissues

Novel analysis of neural outgrowth in 3D human brain micro-tissues
https://main--moleculardevices--hlxsites.hlx.page/en/assets/scientific-posters/dd/img/slas-2023?cmp=7014u000001AW8iAAG&utm_source=internal&utm_medium=newsletter&utm_campaign=md-na-cc-web-internal-20230407-slas2023labnotesblog

Presented by Angeline Lim, PhD

In this study, we reconstructed RealBrain® micro-tissues in 3D and developed a method to quantify the number of neurons and their respective outgrowths. With this approach, we confirmed the efficacy of the compounds with the quantification of neurite outgrowth analysis and found that NGF and PD acid significantly increase, while Rotenone reduces the number of neurites compared to the control. Thus, this type of analysis can be used in multiple applications, such as assessing the effects of compounds on neurons and neural networks.

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Optimizing Animal free CloneDetect assay with Protein G or A to maximize the sensitivity of real-time detection of human IgG antibody production for therapeutic protein engineering and cell line development using ClonePix System

Presented by Paula Zadek, PhD

In this study, we explore the usage of recombinant Pro A or Pro G combined with CloneDetect K8495 to enhance the fluorescent signal for IgG detection/quantification. The results have shown that adding Pro G at the final concentration of 1.0 ug/ml produced the best outcome compared to the control assay without adding Pro G optimizer. The combination of Pro G and CloneDetect K8495 amplified the fluorescent signal and improved detection, due to the stabilization of the complexes formed by the target protein, Pro G, and CloneDetect reagent. This approach can be applied to a variety of cell line models that express proteins that bind to the stabilized complex formed with CloneDetect.

下载海报

https://share.vidyard.com/watch/UZ3eCRAAp4XQ5FAzSdREqA

使用基于阻抗的单细胞分配器和高通量荧光成像仪对细胞系进行单细胞分配和筛选,以执行单克隆性验证

使用基于阻抗的单细胞分配器和高通量荧光成像仪对细胞系进行单细胞分配和筛选,以执行单克隆性验证
https://main--moleculardevices--hlxsites.hlx.page/en/assets/scientific-posters/dd/img/slas-2023?cmp=7014u000001AW8iAAG&utm_source=internal&utm_medium=newsletter&utm_campaign=md-na-cc-web-internal-20230407-slas2023labnotesblog

Presented by Prathyushakrishna Macha, PhD

In this study, we demonstrate a workflow to increase throughput and automate single-cell isolation procedures and clonal outgrowth compared to limiting dilution method. Combining single-cell dispensing and imaging into a workflow provides an innovative and simple impedance-based method for the isolation of intact single cells and imaging. Its incorporation into an automated platform allows the increase in dispensing throughput and reliability and has the potential to facilitate gene editing. In addition, it can generate multiple engineered cell lines that carry specific indel and can facilitate the development of several bioprocess developments or drug screening studies.

下载海报

To summarize, SLAS 2023 was an event full of innovation and collaboration with industry-wide focus squarely on the customers at the center of innovation—and automation is driving this journey. As an industry, we are continuously improving to meet the growing volume and complexity of research projects. Through workflow automation, researchers can increase efficiency, throughput, and experimental confidence to advance discoveries and improve the quality of life everywhere.

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