细胞株开发
稳定细胞株广泛用于许多重要的应用，包括生物制品（如重组蛋白和单克隆抗体）生产、药物筛选和基因功能研究。开发稳定细胞株的过程通常始于将所需的质粒转染至选定的宿主细胞，一般是 CHO 或 HEK 293 细胞。转染后，研究人员随后筛选和定量分析高表达的细胞克隆。一旦检测到这些高产细胞株，便进一步对这些细胞和其产生的蛋白质进行验证。The manual screening methods traditionally used for cell line development are time-consuming and labor-intensive, creating a great demand for high throughput, automated solutions for such efforts.
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细胞膜蛋白表达筛选
许多在细胞表面表达的蛋白都是用于发现和开发生物医药的靶标。例如，G 蛋白偶联受体 (GPCR) 是最大的一类细胞表面蛋白，并且是大约 40% 现有药物的靶标。从转染后的众多细胞中发现和挑选优质的细胞克隆非常具有挑战性。ClonePix 2 系统是可筛选大量细胞的一种自动化方法，它增加了发现稀有高亲和力杂交瘤细胞或高产细胞的概率。
细胞膜蛋白表达筛选
许多在细胞表面表达的蛋白都是用于发现和开发生物医药的靶标。例如，G 蛋白偶联受体 (GPCR) 是最大的一类细胞表面蛋白，并且是大约 40% 现有药物的靶标。从转染后的众多细胞中发现和挑选优质的细胞克隆非常具有挑战性。ClonePix 2 系统是可筛选大量细胞的一种自动化方法，它增加了发现稀有高亲和力杂交瘤细胞或高产细胞的概率。
细胞活力
细胞株开发就是发现源自单个细胞的克隆，其可稳定高表达目标治疗性蛋白。此过程中分离单个活细胞是关键的第一步。单细胞增殖形成集落，随后可评估其目标治疗蛋白的产率。单细胞克隆的活率和生长速率可用 CloneSelect Imager 细胞生长分析系统进行分析。
细胞活力
细胞株开发就是发现源自单个细胞的克隆，其可稳定高表达目标治疗性蛋白。此过程中分离单个活细胞是关键的第一步。单细胞增殖形成集落，随后可评估其目标治疗蛋白的产率。单细胞克隆的活率和生长速率可用 CloneSelect Imager 细胞生长分析系统进行分析。
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Clone productivity screening and titer
识别优质克隆的一个重要步骤是测定单细胞来源克隆的表达量。Screening for productivity using traditional approaches is laborious and time consuming, generally consisting of a multistep process that involves isolating single cells from limiting dilution followed by assessment of titer using ELISA. The ClonePix 2 System combines single-cell isolation and productivity screening into a single step, resulting in dramatically shorter screening times and increased number of candidates. The high throughput Octet systems on the other hand provides a method for the rapid determination of titer that also dramatically shortens screening times.
Clone productivity screening and titer
识别优质克隆的一个重要步骤是测定单细胞来源克隆的表达量。Screening for productivity using traditional approaches is laborious and time consuming, generally consisting of a multistep process that involves isolating single cells from limiting dilution followed by assessment of titer using ELISA. The ClonePix 2 System combines single-cell isolation and productivity screening into a single step, resulting in dramatically shorter screening times and increased number of candidates. The high throughput Octet systems on the other hand provides a method for the rapid determination of titer that also dramatically shortens screening times.
Glycosylation profiling
Glycosylation is an important post-translational modification of biologics drug molecules during production that often has significant impact on product performance and variability. It can affect both safety and efficacy of the biologics molecules hence should be monitored during the screening and selection of these biologics.
Glycosylation profiling
Glycosylation is an important post-translational modification of biologics drug molecules during production that often has significant impact on product performance and variability. It can affect both safety and efficacy of the biologics molecules hence should be monitored during the screening and selection of these biologics.
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单克隆性验证
细胞株开发和单克隆性验证是制备生物制药分子（如单克隆抗体）过程中的关键步骤。在分离稳定表达目标蛋白的单个活细胞后，可建立细胞株。此过程中的一个关键结点是记录单克隆性证据。单克隆性证据通常都是图像形式，据此可生成单细胞图像并将其随附在监管文件中。
单克隆性验证
细胞株开发和单克隆性验证是制备生物制药分子（如单克隆抗体）过程中的关键步骤。在分离稳定表达目标蛋白的单个活细胞后，可建立细胞株。此过程中的一个关键结点是记录单克隆性证据。单克隆性证据通常都是图像形式，据此可生成单细胞图像并将其随附在监管文件中。
单细胞分选
细胞株开发就是发现源自单个细胞的克隆，其可稳定高表达目标治疗性蛋白。此过程的第一步是分离活的单细胞。Limiting dilution is a technique that relies on statistical probability but is time consuming. CloneSelect 单细胞分离系统能够以尽量提高细胞活性的方式来温和分离细胞，同时通过细胞分配时捕获的五个成组图像来提供克隆性的直接证据。
单细胞分选
细胞株开发就是发现源自单个细胞的克隆，其可稳定高表达目标治疗性蛋白。此过程的第一步是分离活的单细胞。Limiting dilution is a technique that relies on statistical probability but is time consuming. CloneSelect 单细胞分离系统能够以尽量提高细胞活性的方式来温和分离细胞，同时通过细胞分配时捕获的五个成组图像来提供克隆性的直接证据。
细胞株开发的资源
彩页
CloneSelect Single-Cell Printer Series
CloneSelect 单细胞打印机系列
The CloneSelect™ Single-Cell Printer™ Series deposit single cells gently and with high efficiency using a patented, inkjet-like disposable, oneway dispensing cartridge.
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彩页
CloneSelect Imager Monoclonality Report Feature
CloneSelect Imager 细胞生长分析系统单克隆性报告功能
在细胞株开发过程中提供单克隆性的图像证明并不像导出单细胞图像一样简单。For example, high-resolution images of the entire well…
应用说明
Rapid automated selection of mammalian cell colonies by cell surface protein expression
通过细胞表面蛋白表达快速自动选择哺乳动物细胞克隆
The ability to select cells based on surface protein expression is of great use in establishing cell lines expressing the receptor(s) of interest for downstream phenotyping/genotyping,…