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SpectraMax Gemini XPS 微孔板读板机

创造自定义的荧光检测

开发新荧光检测, 无需购买昂贵的滤光片组。配有双单色器的顶读 Gemini XPS 微孔板读板机可让您使用各种荧光基团进行实验, 甚至可以为您新的分析测定找出最佳激发和发射波长。多点孔域扫描优化了细胞测定的灵敏度。

优势

  • 在终点法、动力学法、光谱扫描和孔域扫描模式下支持 6 到 384 孔微孔板的样品检测
  • 通过双单色器在 250nm 到 850nm 之间选择激发和发射波长, 无需鉴定、购买和更换滤光片来优化波长
  • 对于终点法和动力学法测量, 可读取最多 4 对波长
  • 从微孔板孔中心的单个点到整个组织培养孔中的多个点进行孔域扫描, 报告出荧光测量结果

测量准确度和重复性

  • 使用 AutoPMT 系统的电子控制功能进行检测的优化, 并避免由于检测器饱和造成的高强度信号或由于重复读取产生的光漂白造成的弱信号的丢失
  • 与其他荧光酶标仪不同, Gemini XPS根据内部标准进行独有的校准, 对比样品间的相对荧光单位 (RFUs)
  • 从环境温度到 45°C 范围内保持稳定的温度调控, 以监测温度敏感性反应
  • 使用用户可设置的 Automix 功能, 充分混匀微孔板中的样品
  • 采用慢速进板设置选项, 减弱对微孔板中细胞或其他聚合物的震动干扰
  • 可与 Molecular Devices 公司专为 SpectraMax 读板机设计的堆板机 StakMax 进行无缝整合, 以组合成自动化工作站

全面的读板机验证

Molecular Devices 微孔读板机拥有最多样的验证工具, 实现客户各种各样的软硬件验证需求。Molecular Devices 通过以下产品和工具提供全面解决方案:

  • 对光吸收、荧光和化学发光模式进行硬件验证的 SpectraTest ABS1FL1LM1 验证板
  • SoftMax Pro 软件验证包
  • 确保符合 FDA 联邦法规第 21 章第 11 款的软件工具

 

Gemini XPS 微孔板读板机支持多种荧光分析测定类别, 包括:

  • ELISA 和免疫分析
  • 核酸 (DNA) 定量
  • 蛋白定量
  • 报告基因检测
  • 细胞活性、增殖和细胞毒性
  • 酶学检测
  • 转运蛋白测定
  • 磷酸酶/激酶
  • 微生物生长

SoftMax® Pro 软件是目前使用最广泛的读板机控制和微孔板数据分析软件, 专为科研工作者的不同要求而设计。软件中含有预设实验模板、分析计算方法和21种不同的曲线拟合方式可选, 为数据获取直到数据分析、论文发表提供完整解决方案。FDA21CFR Part11合规工具可用于SoftMax Pro GxP 软件 , 为标准实验室提供完整解决方案以减少验证成本, 提供终端到终端的监管链, 无需额外的验证分析软件。

一般参数

尺寸 (in.) 8.6(高)×22.8(宽)×15(深)
尺寸 (cm) 22(高)×58(宽)×38(深)
重量 35lb (16kg)
电源 100-240VAC, 50/60Hz

性能参数

波长范围 - 激发 250-850nm
波长范围 - 发射 360-850nm
波长选择 单色器, 1.0nm 步进
波长带宽(激发和发射) 9nm
波长准确度  < ± 2.0nm
顶部读取灵敏度  3.0 fmol/孔 FITC 200µl, 96 孔
光源 氙闪灯

读板时间

一般模式 96 孔 15 秒
384 孔 45 秒
动态范围  6 个数量级
温度范围  环境温度 + 4°C, 最高 45°C
温度均一性(微孔板)  ± 1°C @ 37°C

 

Gemini™ XPS 读板机

 

产品

货号

Gemini XPS 荧光读板机 XPS
SpectraTest FL1 荧光验证板 0200-5060
SpectraDrop™ 超微量检测板基础套装 0200-6262
SpectraDrop™ 超微量板 HTS 版套装 0200-6267
 StakMax 堆板机 StakMax
微孔读板机架 9000-0756

引用次数*:779

最近引用:
如需完整列表, 请点击此处

Increased Vulnerability to Physical Stress by Inactivation of NdgR in Streptomyces coelicolor

BR Lee, DH Yi, E Song, SK Bhatia, JH Lee… - Applied biochemistry …, 2015 - Springer
... Two hundred microliters of the samples was mixed with 1 μL of the 1:1 mixture of SYTO®
9 and PI and incubated for 15 min in dark. The released fluorescence was measured using
Gemini XPS fluorimeter (Molecular Devices, Sunnyvale, CA). ...
 
 

[PDF] The chemokine platelet factor-4 variant (PF-4var)/CXCL4L1 inhibits diabetes-induced blood-retinal barrier breakdown

A El-Asrar, M Ahmed, G Mohammad… - … & visual science, 2015 - jov.arvojournals.org
... Fluorescence was measured using a spectraMax Gemini-XPS microplate reader
(Molecular Devices, Sunnyvale, CA, USA) with excitation and emission wavelengths
of 485 nm and 538 nm, respectively, with PBS as a blank. ...
 

siRNA Delivery Impedes the Temporal Expression of Cytokine-Activated VCAM1 on Endothelial Cells

TT Ho, JO You, DT Auguste - Annals of Biomedical Engineering, 2015 - Springer
... of time were mixed with 100 µL of RNA detecting RiboGreen reagent (Life Technologies, Carlsbad,
CA, USA), incubated at RT for 5 min, then analyzed in 96-well plates using a fluorescence
microplate reader (SpectraMax Gemini XPSMolecular Devices Corp., Sunnyvale, CA ...
 

1‐[2‐(4‐Benzyloxyphenoxy) Ethyl] Imidazole inhibits monoamine oxidase B and protects against neuronal loss and behavioral impairment in rodent models of …

JY Chung, JW Lee, CH Ryu, HK Min… - Journal of …, 2015 - Wiley Online Library
... min. The fluorescence was measured by a fluorescence microplate reader (Gemini
XPS microplate reader; Molecular Devices, Sunnyvale, CA) with a filter set for
excitation and emission at 563 and 587 nm, respectively. The ...
 

Protein Adsorption on a Laser-Modified Titanium Implant Surface

S Cei, D Karapetsa, E Aleo, F Graziani - Implant dentistry, 2015 - journals.lww.com
... Considering that the fluorescence intensity is proportionally related to the amount of protein
adsorbed on the surface, the fluorescence of each treated disc was quantified using a microplate
spectrofluorometer (Spectramax Gemini XPSMolecular Devices, Silicon Valley, CA) set ...
 

Lavandula stoechas essential oil from Spain: Aromatic profile determined by gas chromatography–mass spectrometry, antioxidant and lipoxygenase inhibitory …

A Carrasco, V Ortiz-Ruiz, R Martinez-Gutierrez… - Industrial Crops and …, 2015 - Elsevier
Lavandula stoechas essential oils (EOs), obtained from plants grown in the South East of Spain,
were analyzed by gas chromatography coupled with mass spectromet.
 

Pinacidil protects osteoblastic cells against antimycin A-induced oxidative damage

EM Choi, WW Jung, KS Suh - Molecular medicine …, 2015 - spandidos-publications.com
... ester (AM) for 1 h at 37°C. Following washing with PBS, fluorescence emission (510 nm) was
monitored at the excitation wavelengths of 340 and 380 nm using a Gemini XPS microplate reader
(Molecular Devices, LLC) according to the manufacturer's instructions. ...
 

Epoxy-α-Lapachone Has In Vitro and In Vivo Anti-Leishmania (Leishmania) amazonensis Effects and Inhibits Serine Proteinase Activity in This Parasite

F Souza-Silva, SC Bourguignon… - Antimicrobial agents …, 2015 - Am Soc Microbiol
... Samples were incubated for 60 min at 37°C, and the variance in the relative fluorescence units
(RFU), corresponding to enzymatic cleavage of the substrates, was monitored with a Molecular
Devices SpectraMax spectrophotometer (Gemini XPS) (7). Concomitantly, the efficacy ...
 
 

Inactivation of polyphenol oxidase using 254 nm ultraviolet light in a model system

L Haddouche, A Phalak, RV Tikekar - LWT-Food Science and Technology, 2015 - Elsevier
... However, due to the limitations of the equipment (Gemini XPS fluorescence micro-plate reader,
Molecular Devices, Sunnyvale, CA), enzyme solutions were excited at 280 nm, and the
fluorescence spectra were scanned in a wavelength range of 360–500 nm. ...
 

Sonocatalytic injury of cancer cells attached on the surface of a nickel–titanium dioxide alloy plate

K Ninomiya, H Maruyama, C Ogino, K Takahashi… - Ultrasonics …, 2015 - Elsevier
... Fluorescence signals of 2HTA following the reaction of TA with OH radicals were measured using
a microplate spectrofluorometer (Gemini XPSMolecular Devices, Sunnyvale, CA, USA) at
excitation and emission wavelengths of 315 nm and 425 nm, respectively. ...
 

 

* 截至 2015 年 6 月 26 日。来源:Google Scholar。